Monensin residues in the production of Minas Frescal cheese: Stability, effects on fermentation, fate and physicochemical characteristics of the cheese

Silva, Felipe Rodrigues Nogueira; Pereira, Amanda Dambrós; Baptista, Débora Parra; Pereira, Mararlene Ulberg; Spisso, Bernardete Ferraz; Gigante, Mirna Lúcia; Braga, Patrícia Aparecida de Campos; Reyes, Felix G. R.; Arisseto-Bragotto, Adriana Pavesi

doi:10.1016/j.foodres.2020.109440

Cited by 8 publications

(4 citation statements)

References 30 publications

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“…Furthermore, the residue levels quantified in cheese and whey showed a concentration of this antibiotic in the curd of approximately 5 times, i.e., the antibiotic was concentrated. Additionally, physicochemical parameters and cheese fermentation were not affected by the presence of the antibiotic [ 48 ]. These studies have provided sufficient evidence demonstrating that heat treatments performed on milk, such as pasteurization or sterilization, do not completely eliminate antimicrobial residues.…”

Section: Discussionmentioning

confidence: 99%

Consumption and Informal Trade of Milk in the North of Antioquia (Colombia)

Zapata-Salas,

Guarín,

Ríos-Osorio

2024

Veterinary Medicine International

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The excessive and irrational use of antibiotics to control bovine mastitis and the informal trade in milk with antibiotic residues are objects of great interest for public health, due to the problems associated with the consumption of milk with antibiotic residues on human, animal, and environmental health, and antibiotic resistance. The objective of this study was to understand the attitudes of dairy farmers towards the self-consumption of milk on the farm, the use of milk with antibiotic residues, and the formal and informal milk trade that generates risks for public health. Mixed methods: cross-sectional and grounded theory. Convergent triangulation design. The study was carried out in 9 dairy municipalities in the North of Antioquia, where 216 dairy farmers participated in the quantitative component; and 17 milk producers and 9 veterinarians took part in the qualitative component. A dairy farmer characterization survey was conducted, as well as a survey on the use and marketing of milk from cows with udder health problems and/or under antibiotic treatment. Semistructured interviews were conducted on the same subject. The variable “Intention to sell milk in the village when the dairy industry does not buy it due to high BTSCC” is associated with the BTSCC variable. The variable “Type of marketing reported” is associated with the CFU variable. 5 categories: self-consumption of milk, use of milk with antibiotics, informal milk trade, control of the dairy industry, and beliefs about the elimination of antibiotics in milk, were constructed to theorize about udder health and public health. Sociocultural, political, and economic factors affect decision making in dairy farmers regarding the use and marketing of milk from cows with mastitis and antibiotic residues. These attitudes and behaviors have public health implications.

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Section: Discussionmentioning

confidence: 99%

Consumption and Informal Trade of Milk in the North of Antioquia (Colombia)

Zapata-Salas,

Guarín,

Ríos-Osorio

2024

Veterinary Medicine International

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“…From the 15 th day of the experiment until slaughter of the animals, the finishing diet containing 85% concentrate was provided (Table1). 1 Adap 1 = Adaptation 1, 0-7 days; 2 Adap 2 = Adaptation 2, 7 -14days, 14-89 days; 3 Crude protein (CP); 4 Total digestible nutrients (TDN); 5 Digestible protein intake (DPI); 6 Neutral detergent fiber (NDF); 7 Physically effective neutral detergent fiber (peNFD); 8 Cálcium (Ca); 9 Phosphor (P); 10 Net energy (NE).…”

Section: Animals Facilities Feeding and Animal Carementioning

confidence: 99%

“…Thus, feed additives that eliminate harmful processes of ruminal fermentation are employed, such as sodium monensin, which is a polyester carboxylic ionophore used in growth and finishing diets [5]. in addition to acting bacteriostatically on gram positive ruminal bacteria, but with the possible impact of residues on products of animal origin and microbial resistance [6]. Alternative additives have shown the potential to replace monensin, such as blends of essential oil associated with the exogenous enzyme α-amylase, which has demonstrated gains in performance and carcass weight, in addition to reducing hepatic abscesses and fecal starch in diets with high levels of starch [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Feedlot diets containing increasing starch levels and different feed additives changes cecal proteome profile involved on energy metabolism and inflammatory response of Nellore cattle

Rocha¹,

Assunção²,

Martins³

et al. 2020

Preprint

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Background Diets for feedlot cattle require higher energy density, thus contributing to the high rate of fermentable carbohydrate. The use of feed additives is necessary to reduce possible metabolic disorders. The objective of this study was to analyze the post-rumen effects of different levels of starch (25, 35, and 45%) and additives (Monensin, Blend of essential oil + exogenous α-Amylase) in diets for Nellore cattle feedlot. The cecum tissue proteome was separated by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), and then, with the verification of differentially expressed protein SPOTS, these were characterized by electrospray ionization mass spectrometry (ESI-MS/MS).Results The expression of nine enzymes participating in the Steps of the glycolysis pathway was verified, such as: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Glyceraldehyde-3-phosphate dehydrogenase like-17 protein, Triosephosphate isomerase (Step 1); Phosphoglycerate mutase and Phosphoglycerate mutase 2 (Step 2); Alpha-enolase (ENO1), Beta-enolase (ENO3) and Fructose-bisphosphate aldolase (ALDOB) (Step 4); and Pyruvate Kinase (PKM) (Step 5). There was expression of three enzymes linked to catalytic activities participating in the synthesis of lactate from pyruvate: L-lactate dehydrogenase B, L-lactate dehydrogenase A chain and L-lactate dehydrogenase. The ATP synthase subunit beta and ATP synthase subunit beta_mitochondrial participate in the electron transport chain, producing ATP from ADP in the presence of a proton gradient across the membrane. Due to the manipulation of diets, the expression of the Leukocyte elastase inhibitor protein, associated with the inflammatory response.Conclusions The use of blends of essential oil associated with α-amylase as a feed additive promoted the greater expression of enzymes in the pathway of glycolysis and gluconeogenesis (and the absence of proteins linked to inflammation (Leukocyte elastase inhibitor) in cecum tissues. On the other hand, the increase in starch in the diets promoted a reduction in enzymes linked to carbohydrate degradation with increased responses linked to inflammatory injuries.

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“…Thus, feed additives that decrease harmful ruminal fermentation processes are employed, such as sodium monensin, which is a polyester carboxylic ionophore used in growth and finishing diets 5 . It acts bacteriostatically on Gram-positive ruminal bacteria but may leave residues in products of animal origin and result in microbial resistance 6 . Alternative additives have shown the potential to replace monensin, such as blends of essential oils associated with the exogenous enzyme α-amylase, which has led to demonstrable gains in performance and carcass weight, in addition to reducing hepatic abscesses and fecal starch in animals with high-starch diets 7 – 9 .…”

Section: Introductionmentioning

confidence: 99%

Feedlot diets containing different starch levels and additives change the cecal proteome involved in cattle’s energy metabolism and inflammatory response

Rocha

Assunção

Martins

et al. 2022

Sci Rep

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Diets for feedlot cattle must be a higher energy density, entailing high fermentable carbohydrate content. Feed additives are needed to reduce possible metabolic disorders. This study aimed to analyze the post-rumen effects of different levels of starch (25%, 35%, and 45%) and additives (monensin or a blend of essential oils and exogenous α-amylase) in diets for Nellore feedlot cattle. The cecum tissue proteome was analyzed via two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and then differentially expressed protein spots were identified with liquid chromatography–tandem mass spectrometry (LC–MS/MS). The use of blends of essential oils associated with α-amylase as a feed additive promoted the upregulation of enzymes such as triosephosphate isomerase, phosphoglycerate mutase, alpha-enolase, beta-enolase, fructose-bisphosphate aldolase, pyruvate kinase, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), l-lactate dehydrogenase B, l-lactate dehydrogenase A chain, l-lactate dehydrogenase, and ATP synthase subunit beta, which promote the degradation of carbohydrates in the glycolysis and gluconeogenesis pathways and oxidative phosphorylation, support pyruvate metabolism through the synthesis of lactate from pyruvate, and participate in the electron transport chain, producing ATP from ADP in the presence of a proton gradient across the membrane. The absence of proteins related to inflammation processes (leukocyte elastase inhibitors) in the cecum tissues of animals fed essential oils and amylase may be because feed enzymes can remain active in the intestine and aid in the digestion of nutrients that escape rumen fermentation; conversely, the effect of monensin is more evident in the rumen and less than 10% results in post-ruminal action, corroborating the hypothesis that ionophore antibiotics have a limited effect on the microbiota and intestinal fermentation of ruminants. However, the increase in starch in these diets promoted a downregulation of enzymes linked to carbohydrate degradation, probably caused by damage to the cecum epithelium due to increased responses linked to inflammatory injuries.

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Monensin residues in the production of Minas Frescal cheese: Stability, effects on fermentation, fate and physicochemical characteristics of the cheese

Cited by 8 publications

References 30 publications

Consumption and Informal Trade of Milk in the North of Antioquia (Colombia)

Consumption and Informal Trade of Milk in the North of Antioquia (Colombia)

Feedlot diets containing increasing starch levels and different feed additives changes cecal proteome profile involved on energy metabolism and inflammatory response of Nellore cattle

Feedlot diets containing different starch levels and additives change the cecal proteome involved in cattle’s energy metabolism and inflammatory response

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