Moniezia benedeni infection increases IgE+ cells in sheep (Ovis aries) small intestine

Pan, Jing; Yao, Wan-Ling; Liu, Li-Ping; Wang, Bao-Shan; Chai, Wen-Zhu; Huang, Zhen; Fan, Xi-Ping; He, Wan-Hong; Wang, Wen-Hui; Zhang, Wang-Dong

doi:10.1016/j.vetpar.2024.110169

Cited by 2 publications

(2 citation statements)

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“…After that, boost the immunization every one week (the method and dose were the same as the second time). Six days after the fourth immunization, blood was collected by heart punctures to obtain rabbit anti-sheep SNAP-25 poly-antiserum [ 50 , 51 ].…”

Section: Methodsmentioning

confidence: 99%

“…It is prevalent worldwide, mostly endemic, and it is also an important pathogen of sheep tapeworm disease. Our previous research showed that M. benedeni infection can substantially reduce the densities of IgA + , IgG + , and IgM + cells in the sheep's intestine [ 30 ], and markedly enhance the density of intestinal IgE + cells [ 51 ] and CD3 + T cells [ 53 ] as well as the expression of NMU [ 31 ].However, at present, there are few reports regarding the impact of M. benedeni infection on the expression of SNAP-25 in the sheep's intestine. In this study, the prokaryotic gene expression of SNAP-25 in sheep was investigated, the rabbit anti-sheep SNAP-25 polyclonal antibodies were prepared, and the expression and distribution patterns of SNAP-25 in the sheep's intestine both before and after M. benedeni infection were analyzed.…”

Section: Introductionmentioning

confidence: 99%

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Moniezia benedeni drives the SNAP-25 expression of the enteric nerves in sheep's small intestine

Huang,

Yao,

et al. 2024

BMC Vet Res

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Background The neuroimmune network plays a crucial role in regulating mucosal immune homeostasis within the digestive tract. Synaptosome-associated protein 25 (SNAP-25) is a presynaptic membrane-binding protein that activates ILC2s, initiating the host's anti-parasitic immune response. Methods To investigate the effect of Moniezia benedeni (M. benedeni) infection on the distribution of SNAP-25 in the sheep's small intestine, the recombinant plasmid pET-28a-SNAP-25 was constructed and expressed in BL21, yielding the recombinant protein. Then, the rabbit anti-sheep SNAP-25 polyclonal antibody was prepared and immunofluorescence staining was performed with it. The expression levels of SNAP-25 in the intestines of normal and M. benedeni-infected sheep were detected by ELISA. Results The results showed that the SNAP-25 recombinant protein was 29.3 KDa, the titer of the prepared immune serum reached 1:128,000. It was demonstrated that the rabbit anti-sheep SNAP-25 polyclonal antibody could bind to the natural protein of sheep SNAP-25 specifically. The expression levels of SNAP-25 in the sheep's small intestine revealed its primary presence in the muscular layer and lamina propria, particularly around nerve fibers surrounding the intestinal glands. Average expression levels in the duodenum, jejunum, and ileum were 130.32 pg/mg, 185.71 pg/mg, and 172.68 pg/mg, respectively. Under conditions of M. benedeni infection, the spatial distribution of SNAP-25-expressing nerve fibers remained consistent, but its expression level in each intestine segment was increased significantly (P < 0.05), up to 262.02 pg/mg, 276.84 pg/mg, and 326.65 pg/mg in the duodenum, jejunum, and ileum, and it was increased by 101.06%, 49.07%, and 89.16% respectively. Conclusions These findings suggest that M. benedeni could induce the SNAP-25 expression levels in sheep's intestinal nerves significantly. The results lay a foundation for further exploration of the molecular mechanism by which the gastrointestinal nerve-mucosal immune network perceives parasites in sheep.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Moniezia benedeni drives the SNAP-25 expression of the enteric nerves in sheep's small intestine

Huang,

Yao,

et al. 2024

BMC Vet Res

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Effect of Moniezia Benedeni infection on ileal transcriptome profile characteristics of sheep

Zhang,

Yao,

Meng

et al. 2024

BMC Genomics

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Background The intestinal mucosal immune system, renowned for its precise and sensitive regulation, can provide comprehensive and effective protection for the body, among which the ileum is a critical induction site for regulating mucosal immune homeostasis. Moniezia benedeni parasitizes the small intestine of sheep and can cause serious pathological damage or even death to the host when the infection is severe. In this study, 5 sheep infected with Moniezia benedeni were selected as the infected group, and 5 uninfected sheep were selected as the control group. The ileal transcriptome profile characteristics of Moniezia benedeni infection were analyzed based on RNA-seq sequencing technology, aiming to lay a foundation for further exploring the perception mechanism of sheep intestines to Moniezia benedeni infection and formulating effective prevention and control strategies. Results The results showed that a total of 3,891 differentially expressed genes (DEGs) were detected in the ileum tissues of sheep between the infected and control groups with 2,429 up-regulated genes and 1,462 down-regulated genes. GO and KEGG pathway enrichment analysis of differential genes, as well as Clue GO analysis showed that differential genes were significantly enriched in immune and metabolic-related biological processes and signaling pathways. Particularly, in immune-related signaling pathways, the B cell receptor signaling pathway was significantly down-regulated, while in metabolic regulation related signaling pathways, Bile secretion, Fat digestion and absorption and Vitamin digestion and absorption were notably up-regulated. On this basis, the differential core genes related to immune metabolism were verified by qRT-PCR method. The results showed that OVAR , CD3E , CD8A , CD4 and CD28 were significantly up-regulated ( P < 0.05), while CIITA , BLNK , BCL6 and CD79A were significantly down-regulated ( P < 0.05), which were consistent with transcriptome sequencing data. Conclusions The results demonstrated that Moniezia benedeni infection significantly affected the immune and metabolic processes in sheep ileum, particularly, it significantly inhibited the activation process of host B cells, and also led to an overactive function of bile acid metabolism. This finding provides a solid foundation for further elucidating the response mechanism of Peyer's patches in sheep ileum to Moniezia tapeworm infection. Supplementary Information The online version contains supplementary material available at 10.1186/s128...

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Moniezia benedeni infection increases IgE+ cells in sheep (Ovis aries) small intestine

Cited by 2 publications

References 61 publications

Moniezia benedeni drives the SNAP-25 expression of the enteric nerves in sheep's small intestine

Moniezia benedeni drives the SNAP-25 expression of the enteric nerves in sheep's small intestine

Effect of Moniezia Benedeni infection on ileal transcriptome profile characteristics of sheep

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