Monitoring a Complex Medium Fermentation with Sample-Sample Two-Dimensional FT-NIR Correlation Spectroscopy

Abstract

The possibility of monitoring state transitions during the time course of a fermentation through the analysis of the on-line collected near-infrared (NIR) spectra with sample-sample two-dimensional correlation spectroscopy (SS-2DCoS) was investigated. SS-2DCoS has proved to be useful for extracting process information directly from the spectra collected on-line. The complexity of the system studied prevented the extraction of concentration profiles, but nevertheless, the application of SS-2DCoS enables the identification of fermentation state transitions due to metabolic and morphological changes of the microorganism.

Applying Near‐Infrared Spectroscopy in Downstream Processing: One Calibration for Multiple Clarification Processes of Fermentation Media

“…This API is produced by cultivation of Streptomyces clavuligerus in submerged fermentations. The process and product have been thoroughly described in the literature (3, 4). However, to the best of our knowledge, no reports on the use of NIR on DSP clarification processes have been reported.…”

Modelling Industrial Fermentation Data With Multiway Multivariate Techniques

“…It is possible that the changes identified at 20 and 72 h of growth are due to morphological alterations in the microorganism (cf. Ferreira et al (2006)) but there is no clear evidence to support this hypothesis.…”

Online‐Infrarotspektroskopie in der Bioprozessanalytik

“…Die Möglichkeit einer Bestimmung von Zustandsübergängen infolge metabolischer und morphologischer Veränderungen der Mikroorganismen während einer Fermentation durch die Analyse von online-NIR-Spektren durch SS-2DCoS (sample-sample 2-dimensional correlation spectroscopy) wurde von Ferreira et al [20] …”