Monitoring Ambient Air for Mutagenicity Using the Higher Plant Tradescantia

Schairer, L.A.; Sautkulis, Richard C.; Tempel, Neal

doi:10.1007/978-1-4613-3455-2_11

Cited by 13 publications

(5 citation statements)

References 22 publications

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“…One of the most commonly used bioassays, also considered extremely reliable, for evaluating genotoxicity is the pink mutation assay with Tradescantia staminal hairs. This test is highly valued for its simplicity, and it is used to detect the effect of a wide spectrum of chemical agents and complex mixtures ( Underbrink et al , 1973 ; Schairer et al , 1982 ; Ahmed and Grant, 1992 ; Grant and Salamone, 1994 ; Ma et al , 1994 ; Sandhu et al , 1994 ). In this assay, color changes in cells from floral parts are used to determine mutational events.…”

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confidence: 99%

Evaluation of genetic damage induced by glyphosate isopropylamine salt using Tradescantia bioassays

et al. 2010

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Glyphosate is noted for being non-toxic in fishes, birds and mammals (including humans). Nevertheless, the degree of genotoxicity is seriously controversial. In this work, various concentrations of a glyphosate isopropylamine salt were tested using two methods of genotoxicity assaying, viz., the pink mutation assay with Tradescantia (4430) and the comet assay with nuclei from staminal cells of the same plant. Staminal nuclei were studied in two different forms, namely nuclei from exposed plants, and nuclei exposed directly. Using the pink mutation assay, isopropylamine induced a total or partial loss of color in staminal cells, a fundamental criterion utilized in this test. Consequently, its use is not recommended when studying genotoxicity with agents that produce pallid staminal cells. The comet assay system detected statistically significant (p < 0.01) genotoxic activity by isopropylamine, when compared to the negative control in both the nuclei of treated plants and directly treated nuclei, but only the treated nuclei showed a dose-dependent increase. Average migration in the nuclei of treated plants increased, when compared to that in treated nuclei. This was probably due, either to the permanence of isopropylamine in inflorescences, or to the presence of secondary metabolites. In conclusion, isopropylamine possesses strong genotoxic activity, but its detection can vary depending on the test systems used.

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confidence: 99%

Evaluation of genetic damage induced by glyphosate isopropylamine salt using Tradescantia bioassays

et al. 2010

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“…The pictures represent stamen hair cells of Tradescantia clone 4430 plant that were captured during the Trad-SHM bioassay. This assay is indicated to identify changes in cell color from blue to pink, which indicates that the plant was exposed to some types of environmental stress, such as temperature or pH change, radiation, and contamination of soil, water, or air (Underbrink et al, 1973 ; Sparrow et al, 1974 ; Schairer et al, 1978 , 1982 ; Ma et al, 1994 ). This change in color can be a result of mutation (Meravi and Prajapati, 2018 ) and/or can be associated with anthocyanin pigments that are responsible for red, purple, and blue colors, and they act as antioxidants and may play a role in protecting plants from damage caused by UV light, pathogens, and herbivores (Nassour et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

Tradescantia response to air and soil pollution, stamen hair cells dataset and ANN color classification

Rodrigues,

Goeldner,

Mercuri

et al. 2024

Front. Big Data

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Tradescantia plant is a complex system that is sensible to environmental factors such as water supply, pH, temperature, light, radiation, impurities, and nutrient availability. It can be used as a biomonitor for environmental changes; however, the bioassays are time-consuming and have a strong human interference factor that might change the result depending on who is performing the analysis. We have developed computer vision models to study color variations from Tradescantia clone 4430 plant stamen hair cells, which can be stressed due to air pollution and soil contamination. The study introduces a novel dataset, Trad-204, comprising single-cell images from Tradescantia clone 4430, captured during the Tradescantia stamen-hair mutation bioassay (Trad-SHM). The dataset contain images from two experiments, one focusing on air pollution by particulate matter and another based on soil contaminated by diesel oil. Both experiments were carried out in Curitiba, Brazil, between 2020 and 2023. The images represent single cells with different shapes, sizes, and colors, reflecting the plant's responses to environmental stressors. An automatic classification task was developed to distinguishing between blue and pink cells, and the study explores both a baseline model and three artificial neural network (ANN) architectures, namely, TinyVGG, VGG-16, and ResNet34. Tradescantia revealed sensibility to both air particulate matter concentration and diesel oil in soil. The results indicate that Residual Network architecture outperforms the other models in terms of accuracy on both training and testing sets. The dataset and findings contribute to the understanding of plant cell responses to environmental stress and provide valuable resources for further research in automated image analysis of plant cells. Discussion highlights the impact of turgor pressure on cell shape and the potential implications for plant physiology. The comparison between ANN architectures aligns with previous research, emphasizing the superior performance of ResNet models in image classification tasks. Artificial intelligence identification of pink cells improves the counting accuracy, thus avoiding human errors due to different color perceptions, fatigue, or inattention, in addition to facilitating and speeding up the analysis process. Overall, the study offers insights into plant cell dynamics and provides a foundation for future investigations like cells morphology change. This research corroborates that biomonitoring should be considered as an important tool for political actions, being a relevant issue in risk assessment and the development of new public policies relating to the environment.

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“…This clone is a diploid hybrid (2n = 12) between a blue-flowered T. hirsutiflora Bush and a pink-flowered T. subacaulis Bush (Emmerling-Thompson and Nawrocky, 1980), and thus is a blue/pink heterozygote. This clone has frequently been used in studies of somatic mutations in the stamen hairs, as reviewed earlier (Schairer and Sautkulis, 1982;Schairer et al, 1983;Ichikawa, 1992), and is very suitable for propagating and growing the young inflorescence-bearing shoots with roots because of its characteristics, i.e., many new shoots constantly emerging from the basal nodes one after another and its short height .…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…The Tradescantia stamen-hair system, selected by the International Program on Plant Bioassays as one of the most suitable testers for detecting genotoxic hazards in the environment, has been used successfully to detect the genetic effects of ionizing radiations and various chemicals at low levels, as reviewed earlier (Underbrink et al, 1973;Ichikawa, 1981bIchikawa, , 1992Schairer and Sautkulis, 1982;Schairer et al, 1983;Ma et al, 1994). The system has also been shown to be suitable for studying the variation in spontaneous somatic mutation frequency (Takahashi and Ichikawa, 1976;Schairer and Sautkulis, 1982;Schairer et al, 1983;Ichikawa 1984Ichikawa , 1992Ichikawa , 1994Imai et al, 1991;Sanda-Kamigawara et al, 1991Ichikawa et al, 1995Ichikawa et al, , 1996aIchikawa et al, , 1996bIchikawa and Wushur, 2000). The use of the young inflorescencebearing shoots with roots of clone BNL 4430 cultivated in a recently developed nutrient solution circulating (NSC) growth chamber (Shima and Ichikawa, 1994;Ichikawa and Wushur, 2000) has been shown to be especially efficient for detecting mutagenic synergisms (Shima and Ichikawa, 1994Xiao and Ichikawa, 1995, 1996, 1998a, 1998b or antagonisms (Xiao and Ichikawa, 1995, 1996, 1998a, 1998c among various chemicals and X-rays, making it possible to determine spontaneous background mutation frequencies with higher accuracy than before Ichikawa and Wushur, 2000).…”

Section: Introductionmentioning

confidence: 99%

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Further yearly analyses of spontaneous pink mutant events in the stamen hairs of Tradescantia clone BNL 4430 cultivated in the NSC growth chamber.

Ichikawa

Wushur

2001

Genes Genet. Syst.

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In order to confirm the results obtained in the previous 1-year-term (December 12, 1998, through December 10, 1999) scorings and analyses of spontaneous pink mutant events (PMEs) in the stamen hairs of Tradescantia clone BNL 4430 cultivated in a nutrient solution circulating (NSC) growth chamber, similar scorings and analyses were continued for another 52-week period from December 11, 1999, through December 8, 2000. The environmental conditions were not changed, except for a minor modification in the method of supplying the nutrient solution used. During the scoring period, 732,128 stamen hairs with an average cell number of 24.90 cells were observed, and 2,368 PMEs were detected. The overall spontaneous somatic mutation frequency was 1.35 +/- 0.03 PMEs per 10(4) hair-cell divisions, which was significantly lower than the value of 1.56 +/- 0.03 determined in the previous 52-week period, and the frequencies were lower during April through September than in other months, the period showing lower frequencies lasting 1-month longer than in the previous year. The present results reconfirmed the occurrence of a clear seasonal variation in the spontaneous mutation frequency in the NSC growth chamber, and the lower overall frequency, probably related to the minor modification in supplying the nutrient solution, is helpful for conducting mutagenicity tests at low levels, offering a lower background level. The analyses of the sectoring patterns of all these PMEs showed that the most of the 203 cases of multiple (two to five) pink sectors observed in the same stamen hairs (scored as 253 PMEs for calculating mutation frequency) were the results of events involving somatic recombinations occurred in single cells or cell lineages, rather than those of two or more independent somatic mutations occurred in different cells, agreeing with our previous study, and the significance of somatic recombinations in causing single PMEs was also reconfirmed.

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Monitoring Ambient Air for Mutagenicity Using the Higher Plant Tradescantia

Cited by 13 publications

References 22 publications

Evaluation of genetic damage induced by glyphosate isopropylamine salt using Tradescantia bioassays

Evaluation of genetic damage induced by glyphosate isopropylamine salt using Tradescantia bioassays

Tradescantia response to air and soil pollution, stamen hair cells dataset and ANN color classification

Further yearly analyses of spontaneous pink mutant events in the stamen hairs of Tradescantia clone BNL 4430 cultivated in the NSC growth chamber.

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