Monitoring and contamination incidence of gnotobiotic experiments performed in microisolator cages

Basic, Marijana; Bolsega, Silvia; Smoczek, Anna; Gläsner, Joachim; Hiergeist, Andreas; Eberl, Claudia; Stecher, Bärbel; Gessner, André

doi:10.1016/j.ijmm.2021.151482

Cited by 9 publications

(4 citation statements)

References 27 publications

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“…They were maintained in plastic film isolators (Metall+Plastik GmbH, Radolfzell-Stahringen, Germany) in a controlled environment and twelve-hour light/dark cycles. Hygiene monitoring according to standard operating procedures 70,71 confirmed that the mice were free of contaminants or infection with common murine pathogens throughout the experiment. GF mice (n = 3 males and 3 females per group) were removed from the breeding isolator at the age of five weeks and colonized with the corresponding OMM stocks, SPF microbiota, or left untreated (GF controls).…”

Section: Methodsmentioning

confidence: 94%

Section: Mouse Experiments With Oligo-mouse Microbiota (Omm)mentioning

confidence: 94%

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Enhanced cultured diversity of the mouse gut microbiota enables custom-made synthetic communities

Afrizal

Jennings

Hitch

et al. 2022

Preprint

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Microbiome research is hampered by the fact that many bacteria are still unknown and by the lack of publicly available isolates. Fundamental and clinical research is in need of comprehensive and well-curated repositories of cultured bacteria from the intestine of mammalian hosts. In this work, we expanded the mouse intestinal bacterial collection (www.dsmz.de/miBC) to 212 strains, all publicly available and taxonomically described. This includes the study of strain-level diversity, small-sized bacteria, and the isolation and characterization of the first cultured members of one novel family, 10 novel genera, and 39 novel species. We demonstrate the value of this collection by performing two studies. First, metagenome-educated design of synthetic communities (SYNs) allowed establishing custom strain consortia that reflect different susceptibilities to DSS-induced colitis. Second, nine phylogenetically and functionally diverse species were used to amend the Oligo-Mouse Microbiota (OMM)12 model [Brugiroux et al. 2016 Nat Microbiol]. These strains compensated for differences observed between gnotobiotic OMM12 and specific-pathogen free (SPF) mice at multiple levels, including body composition and immune cell populations (e.g., induction of T-cell subtypes) in the intestine and associated lymphoid tissues. Ready-to-use OMM stocks from this work are available to the community for use in future studies. In conclusion, this work improves our knowledge of gut microbiota diversity in mice and enables functional studies via the modular use of isolates.

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Section: Methodsmentioning

confidence: 94%

Section: Mouse Experiments With Oligo-mouse Microbiota (Omm)mentioning

confidence: 94%

Enhanced cultured diversity of the mouse gut microbiota enables custom-made synthetic communities

Afrizal

Jennings

Hitch

et al. 2022

Preprint

Self Cite

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“…Breeding colonies of animals colonized with defined microbial species are additionally screened by 16S rRNA-based microbe-specific qPCR and next-generation sequencing to confirm the presence of intentionally introduced microorganisms and to exclude the presence of unknown/unwanted contaminants. 65 Routine microbiological monitoring according to the FELASA recommendations 64 and recommendations for maintaining gnotobiotic colonies 66 did not reveal any evidence of infection with common murine pathogens in specific pathogen-free animals or contaminants in GF and gnotobiotic animals.…”

Section: Methodsmentioning

confidence: 96%

Gut microbiota depletion delays somatic peripheral nerve development and impairs neuromuscular junction maturation

Cescon,

Gambarotta,

Calabrò

et al. 2024

Gut Microbes

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“…Several studies have shown that introduced microbes including viruses and bacteria can successfully colonize the gut of ASF-associated mice [23][24][25]. This implies that these mice are also highly susceptible to contamination by environmental bacteria and fungi [26], especially if aseptic condition in animal handling by scientists and animal caretakers is not strictly kept. Even though the ASF is widely used in biomedical research, individual bacterial species are not available in public strain collections, complicating the study-based modification of the consortium.…”

Section: Syncoms Representing Simplified Intestinal Microbiomementioning

confidence: 99%

Synthetic Microbiomes on the Rise—Application in Deciphering the Role of Microbes in Host Health and Disease

Bolsega

Bleich

2021

Nutrients

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The intestinal microbiota conveys significant benefits to host physiology. Although multiple chronic disorders have been associated with alterations in the intestinal microbiota composition and function, it is still unclear whether these changes are a cause or a consequence. Hence, to translate microbiome research into clinical application, it is necessary to provide a proof of causality of host–microbiota interactions. This is hampered by the complexity of the gut microbiome and many confounding factors. The application of gnotobiotic animal models associated with synthetic communities allows us to address the cause–effect relationship between the host and intestinal microbiota by reducing the microbiome complexity on a manageable level. In recent years, diverse bacterial communities were assembled to analyze the role of microorganisms in infectious, inflammatory, and metabolic diseases. In this review, we outline their application and features. Furthermore, we discuss the differences between human-derived and model-specific communities. Lastly, we highlight the necessity of generating novel synthetic communities to unravel the microbial role associated with specific health outcomes and disease phenotypes. This understanding is essential for the development of novel non-invasive targeted therapeutic strategies to control and modulate intestinal microbiota in health and disease.

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Monitoring and contamination incidence of gnotobiotic experiments performed in microisolator cages

Cited by 9 publications

References 27 publications

Enhanced cultured diversity of the mouse gut microbiota enables custom-made synthetic communities

Enhanced cultured diversity of the mouse gut microbiota enables custom-made synthetic communities

Gut microbiota depletion delays somatic peripheral nerve development and impairs neuromuscular junction maturation

Synthetic Microbiomes on the Rise—Application in Deciphering the Role of Microbes in Host Health and Disease

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