Monitoring and fast detection of mycotoxin-producing fungi based on headspace solid-phase microextraction and headspace sorptive extraction of the volatile metabolites

Demyttenaere, Jan; Moriña, Rosa M.; Sandra, Pat

doi:10.1016/s0021-9673(02)01417-6

Cited by 68 publications

(40 citation statements)

References 24 publications

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“…Among the identified volatiles from Penicillium roqueforti were the sesquiterpenes -patchoulene (95), a -elemene-isomer, -elemene (14), diepi-α-cedrene (97), -gurjunene (103), a -patchouleneisomer, aristolochene (58), valencene (59), α-selinene (48), -himachalene (64), α-chamigrene (77), -bisabolene (18) and α-panasinsene (98) (Jelen 2002). In another study these sesquiterpene hydrocarbons were confirmed and in addition caryophyllene (61), -chamigrene (78) and germacrene A (10) were reported (Demyttenaere et al 2003). The authors could also show that P. roquefortii strains producing PR toxin and sporogen AO-1 produced also high amounts of aristolochene while toxin-free strains are characterized by high amounts of two unidentified sesquiterpene hydrocarbons.…”

Section: Two Unidentified Sesquiterpene Hydrocarbons Have Been Detectmentioning

confidence: 92%

Volatile sesquiterpenes from fungi: what are they good for?

2011

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Section: Two Unidentified Sesquiterpene Hydrocarbons Have Been Detectmentioning

confidence: 92%

Volatile sesquiterpenes from fungi: what are they good for?

2011

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“…Sampling methods, such as thermal desorption tube (Tenax TA) [4,26,27], purge and trapping of headspace gases [28,29], headspace sorptive extraction [12,30] and solid phase microextraction (SPME) [31][32][33][34], have been used for the collection of MVOCs. SPME is a popular technique because it has the advantages of low cost per analysis and portability.…”

Section: Introductionmentioning

confidence: 99%

Effects of Growth Parameters on the Analysis of Aspergillus flavus Volatile Metabolites

et al. 2016

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Aspergillus flavus produces dangerous secondary metabolites known as aflatoxins, which are toxic and carcinogenic, and their contamination of agricultural products results in health issues and economic hardships in the U.S. and around the world. Early identification of aflatoxigenic isolates of A. flavus is the key in the management of these fungi. An emerging detection method for specific fungi identification involves the analysis of microbial volatile organic compounds (MVOCs) released by the fungi. Complicating this approach is the understanding that many factors influence metabolic production, including growth parameters, such as growth media, temperature, spore counts and oxidation stress. In addition, analytical and data analysis methods can also influence the results. Several growth and analysis methods were evaluated and optimized in order to better understand the effect of the methods on fungi MVOC signatures. The results indicate that carboxen/polydimethylsiloxane (CAR/PDMS) has the best extraction efficiency for the MVOCs emitted by A. flavus. Both chemical defined agar (CDA) and chemical defined liquid (CDL) are suitable growth media for MVOC emission studies. The highest MVOC production was found at 30˝C. Log transformation was considered one of the best data pretreatment methods when analyzing MVOC data and resulted in the best principal component analysis (PCA) clustering in the experiments with different growth media. This study aims to elucidate fungal volatile organic compounds (VOCs) differences due to variations in growth parameters as a first step in the development of an analytical method for the monitoring of aflatoxigenic A. flavus contamination in crop storage facilities.

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“…Thermal desorption tubes have been used for field sample collection followed by transportation to a lab for analysis [16] [20]. Solid phase microextraction (SPME) has been used to collect and concentrate MVOCs from fungi and bacteria [21]. This technique has the potential to be part of an efficient method for field applications due to its portability and simplicity.…”

Section: Introductionmentioning

confidence: 99%

Monitoring MVOC Profiles over Time from Isolates of Aspergillus flavus Using SPME GC-MS

Sun¹,

Wood-Jones²,

Wang³

et al. 2014

JACEN

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Fungi produce a variety of microbial volatile organic compounds (MVOCs) during primary and secondary metabolism. The fungus, Aspergillus flavus, is a human, animal and plant pathogen which produces aflatoxin, one of the most carcinogenic substances known. In this study, MVOCs were analyzed using solid phase microextraction (SPME) combined with GCMS from two genetically different A. flavus strains, an aflatoxigenic strain, NRRL 3357, and a non-aflatoxigenic strain, NRRL 21882. A PDMS/CAR SPME fiber was used over 30 days to observe variations in MVOCs over time. The relative percentage of individual chemicals in several chemical classes (alcohols, aldehydes, esters, furans, hydrocarbons, ketones, and organic acids) was shown to change considerably during the varied fungal growth stages. This changing chemical profile reduces the likelihood of finding a single chemical that can be used consistently as a biomarker for fungal strain identification. In our study, discriminant analysis techniques were successfully conducted using all identified and quantified MVOCs enabling discrimination of the two A. flavus strains over the entire 30-day period. This study underscores the potential of using SPME GCMS coupled with multivariate analysis for fungi strain identification.

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Monitoring and fast detection of mycotoxin-producing fungi based on headspace solid-phase microextraction and headspace sorptive extraction of the volatile metabolites

Cited by 68 publications

References 24 publications

Volatile sesquiterpenes from fungi: what are they good for?

Volatile sesquiterpenes from fungi: what are they good for?

Effects of Growth Parameters on the Analysis of Aspergillus flavus Volatile Metabolites

Monitoring MVOC Profiles over Time from Isolates of Aspergillus flavus Using SPME GC-MS

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