Monitoring binding affinity between drug and α1-acid glycoprotein in real time by Venturi easy ambient sonic-spray ionization mass spectrometry

Liu, Ning; Lu, Xin; Yang, Yuhan; Yao, Chen Xi; Ning, Baoming; He, Dacheng; Ouyang, Jin

doi:10.1016/j.talanta.2015.04.047

Cited by 13 publications

(8 citation statements)

References 29 publications

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“…MS is nowadays applicable nearly by everyone, to every sample and wherever it is needed. These universal characteristics of modern MS have become possible mostly due to the development of new and revolutionary ionization techniques, which are able to handle molecules with a large diversity of size and polarities: from atoms and small molecules such as benzene and pharmaceutical drugs such as acetyl salicylic acid and salts such as ionic liquids to large (bio)molecules as well as tissues, proteins, embryos, viruses, and intact bacteria . The emergence of these general and mild ionization techniques have also contributed to the simplicity of MS analysis and its “everything-everyone-everywhere” use.…”

Section: Ambient Mass Spectrometrymentioning

confidence: 99%

Forensic Chemistry and Ambient Mass Spectrometry: A Perfect Couple Destined for a Happy Marriage?

et al. 2016

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Ambient mass spectrometry has been demonstrated, via various proof-of-concept studies, to offer a powerful, rather universal, simple, fast, nondestructive, and robust tool in forensic chemistry, producing reliable evidence at the molecular level. Its nearly nondestructive nature also preserves the sample for further inquiries. This feature article demonstrates the applicability of ambient mass spectrometry in forensic chemistry and explains the challenges that need to be overcome for this technique to make the ultimate step from the academic world into forensic institutes worldwide. We anticipate that the many beneficial and matching figures of merit will bring forensic chemistry and ambient mass spectrometry to a long-term relationship, which is likely to get strongly consolidated over the years.

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Section: Ambient Mass Spectrometrymentioning

confidence: 99%

Forensic Chemistry and Ambient Mass Spectrometry: A Perfect Couple Destined for a Happy Marriage?

et al. 2016

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“…The famous Venturi effect [30] has already been used in a few self-pumping devices for conventional ESI-MS [31] and was proposed for AMS by Santos and coworkers [32,33] based on sonic-spray ionization (SSI). In this work, we designed a Venturi self-pumping-based device and combined it with EESI-MS.…”

Section: Rationalementioning

confidence: 99%

“…This time-consuming operation brings concern about sample contamination, and the stability of results strongly depends on the proficiency of the operator. Furthermore, the sample volume is too small to make a continuous analysis.The famous Venturi effect [30] has already been used in a few self-pumping devices for conventional ESI-MS [31] and was proposed for AMS by Santos and coworkers [32,33] based on sonic-spray ionization (SSI). In this work, we designed a Venturi self-pumping-based device and combined it with EESI-MS.…”

mentioning

confidence: 99%

Self‐aspiration sampling extractive electrospray ionization mass spectrometry (EESI‐MS) for high‐throughput analysis of liquid samples

Jia

Shuai

Liang

et al. 2016

Rapid Comm Mass Spectrometry

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RATIONALE:Extractive electrospray ionization mass spectrometry (EESI-MS) was invented as a typical ambient mass spectrometry method (AMS) and has been used for analyzing complex liquid samples. Here, we designed a Venturi effect-based self-aspiration sampling device and applied it to the EESI-MS for high-throughput analysis of liquid sample. METHODS: A special concentric nebulizer was designed and employed to produce a suction force for the direct aspiration of liquid samples, followed by ionization and detection. This sample aspiration process was explained and optimized using computational fluid dynamics (CFD) analysis. Experiment data were recorded to exhibit the sensitivity, memory effect, inter-day reproducibility, throughput, and applicability of the self-aspiration sampling EESI-MS. RESULTS: The limit of detection (LOD) of this method was determined as 4.5 × 10 À10 g/mL (S/N = 3) for caffeine, and the sample throughput and relative standard deviation (RSD) for full scan mode can reach 0.67 samples/s and 4.76%, respectively. Even for MS/MS mode, the frequency can still be kept at 0.4 samples/s (RSD = 4.71%). Inter-day RSD examined in 1 week was below 10% for the signal of characteristic fragment ions of reserpine. Moreover, based on this method, the amount of caffeine in instant coffee was determined as 4.7%. This device was also proven to be suitable for the protein/peptide analysis. CONCLUSIONS: These experiment results, especially the amazing results on sample throughput and inter-day RSD, suggest that we provide a valuable device which can be used for the direct high-throughput qualitative/quantitative mass spectrometry analysis of real liquid samples in ambient. Copyright © 2016 John Wiley & Sons, Ltd.Ambient mass spectrometry (AMS) [1][2][3] has promoted the development of mass spectrometry (MS)-based high-throughput screening technology, [4][5][6] for its ability in rapid and direct analysis of various samples. Desorption electrospray ionization (DESI), [7][8][9][10] the first reported ambient ionization method, has been demonstrated in the high-throughput analysis of pharmaceutical samples, [11] immediately after its invention. During the same time period, direct analysis in real time (DART) [12][13][14][15] was invented, and several sample injection devices based on DART have been developed to increase the method's detection speed and throughput. [16,17] Both DESI and DART are mainly used to detect volatile, semi-volatile, or easily desorbed compounds from a solid surface, but they are inconvenient for liquid samples which need to be smeared on special surfaces.Extractive electrospray ionization (EESI) was invented originally for the direct detection of complex liquid samples [18,19] and has recently been successfully used in the analysis of exhaled gasses, [20,21] viscous samples, [22][23][24] solid surfaces, [25][26][27][28] etc. With an improved nanoEESI-MS method, Hu et al. [29] achieved the rapid analysis of a single sample within only 1.2 s. However, in nanoEESI-MS experiments, the solution ...

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“…Taking advantage of the Venturi effect, several autopumping devices have been developed for the transfer of various samples to mass spectrometers. − Compared with the sampling of air contaminants or screening of enzyme inhibitors, the detection of secretory products of live cells requires efficient ionization under physiological conditions at near neutral pH without the application of high voltages. Sonic spray ionization is an alternative without the use of a high voltage. − Although a sheath gas flow at sonic speed can create a statistical imbalance of charges on droplets which eventually create gaseous ions, the smaller number of charged species compared to the high voltage-driven ESI limits the detection of low-abundance substances. − Importantly, because ion losses in the nonfocused spraying plume are unavoidable in all spraying techniques, new methods are needed for quantitative and sensitive monitoring of the kinetics of intercellular secretions.…”

Section: Introductionmentioning

confidence: 99%

Cell-Based Ambient Venturi Autosampling and Matrix-Assisted Laser Desorption Ionization Mass Spectrometric Imaging of Secretory Products

et al. 2022

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A cell-based ambient Venturi autosampling device was established for the monitoring of dynamic cell secretions in response to chemical stimulations in real time with temporal resolution on the order of a second. Detection of secretory products of cells and screening of bioactive compounds are primarily performed on an ambient autosampling probe and matrix-assisted laser desorption ionization (MALDI) mass spectrometry. It takes advantage of the Venturi effect in which the fluid flowing through an inlet capillary tube is automatically fed into a parallel array of multiple outlet capillaries. Cells are incubated inside the inlet capillary tube that is connected with either a syringe pump or liquid chromatography (LC) for the transfer of single compounds or mixtures, respectively. Secretory products were continuously pushed into the outlet capillaries and then spotted into a compressed thin film of the matrix material 9-aminoacridine for MALDI mass spectrometric imaging. In physiological pH, without the use of high voltages and without the use of chemical derivatizations, this platform can be applied to the direct assay of neurotransmitters or other secretory products released from cells in response to the stimulation of individual compounds or LC-separated eluates of natural mixtures. It provides a new way to identify bioactive compounds with a detection limit down to 0.04 fmol/pixel.

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Monitoring binding affinity between drug and α1-acid glycoprotein in real time by Venturi easy ambient sonic-spray ionization mass spectrometry

Cited by 13 publications

References 29 publications

Forensic Chemistry and Ambient Mass Spectrometry: A Perfect Couple Destined for a Happy Marriage?

Forensic Chemistry and Ambient Mass Spectrometry: A Perfect Couple Destined for a Happy Marriage?

Self‐aspiration sampling extractive electrospray ionization mass spectrometry (EESI‐MS) for high‐throughput analysis of liquid samples

Cell-Based Ambient Venturi Autosampling and Matrix-Assisted Laser Desorption Ionization Mass Spectrometric Imaging of Secretory Products

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