Monitoring dexamethasone skin biodistribution with ex vivo MALDI-TOF mass spectrometry imaging and confocal Raman microscopy

Pena-Rodríguez, Eloy; García‐Berrocoso, Teresa; Fernández, Ezequiel Vázquez; Otero-Espinar, Francisco J.; Abián, Joaquín; Fernández-Campos, Francisco

doi:10.1016/j.ijpharm.2023.122808

Cited by 6 publications

(1 citation statement)

References 40 publications

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“…Conventional CRM uses spontaneous Raman scattering for the chemical determination and characterization of skin (preferentially SC) constituents’ [ 184 , 187 ] and physiological parameters’ depth profiles [ 188 ], in the numerous in vivo and ex vivo skin penetration studies [ 31 , 37 , 189 , 190 , 191 , 192 , 193 , 194 , 195 , 196 , 197 , 198 , 199 , 200 , 201 , 202 ] and the study of the influence of cosmetics on the physiological parameters of the SC in vivo [ 203 , 204 , 205 ]—a challenging task in skin research [ 186 ]. The unique features of the CRM are its non-invasiveness for skin constituents and target substances, the lack of need for fluorescent or other markers, and the ability to perform chemical analyses quantitatively using various calibration approaches [ 206 , 207 , 208 , 209 , 210 , 211 , 212 , 213 , 214 ]. In most cases, red and near-infrared excitation wavelengths are used, which have low absorption and scattering in the skin [ 215 ] (“skin optical transparent window-I” ( Figure 3 )) and, therefore, penetrate deep and excite the Raman spectra of skin constituents and applied substances with a very low fluorescence intensity, making the Raman bands easy to determine.…”

Section: Confocal Raman Micro-spectroscopy (Crm)mentioning

confidence: 99%

Optical Methods for Non-Invasive Determination of Skin Penetration: Current Trends, Advances, Possibilities, Prospects, and Translation into In Vivo Human Studies

Darvin

2023

Pharmaceutics

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Information on the penetration depth, pathways, metabolization, storage of vehicles, active pharmaceutical ingredients (APIs), and functional cosmetic ingredients (FCIs) of topically applied formulations or contaminants (substances) in skin is of great importance for understanding their interaction with skin targets, treatment efficacy, and risk assessment—a challenging task in dermatology, cosmetology, and pharmacy. Non-invasive methods for the qualitative and quantitative visualization of substances in skin in vivo are favored and limited to optical imaging and spectroscopic methods such as fluorescence/reflectance confocal laser scanning microscopy (CLSM); two-photon tomography (2PT) combined with autofluorescence (2PT-AF), fluorescence lifetime imaging (2PT-FLIM), second-harmonic generation (SHG), coherent anti-Stokes Raman scattering (CARS), and reflectance confocal microscopy (2PT-RCM); three-photon tomography (3PT); confocal Raman micro-spectroscopy (CRM); surface-enhanced Raman scattering (SERS) micro-spectroscopy; stimulated Raman scattering (SRS) microscopy; and optical coherence tomography (OCT). This review summarizes the state of the art in the use of the CLSM, 2PT, 3PT, CRM, SERS, SRS, and OCT optical methods to study skin penetration in vivo non-invasively (302 references). The advantages, limitations, possibilities, and prospects of the reviewed optical methods are comprehensively discussed. The ex vivo studies discussed are potentially translatable into in vivo measurements. The requirements for the optical properties of substances to determine their penetration into skin by certain methods are highlighted.

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Section: Confocal Raman Micro-spectroscopy (Crm)mentioning

confidence: 99%

Optical Methods for Non-Invasive Determination of Skin Penetration: Current Trends, Advances, Possibilities, Prospects, and Translation into In Vivo Human Studies

Darvin

2023

Pharmaceutics

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Advancing rare cancer research by MALDI mass spectrometry imaging: Applications, challenges, and future perspectives in sarcoma

Stillger,

Li,

Hönscheid

et al. 2024

Proteomics

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MALDI mass spectrometry imaging (MALDI imaging) uniquely advances cancer research, by measuring spatial distribution of endogenous and exogenous molecules directly from tissue sections. These molecular maps provide valuable insights into basic and translational cancer research, including tumor biology, tumor microenvironment, biomarker identification, drug treatment, and patient stratification. Despite its advantages, MALDI imaging is underutilized in studying rare cancers. Sarcomas, a group of malignant mesenchymal tumors, pose unique challenges in medical research due to their complex heterogeneity and low incidence, resulting in understudied subtypes with suboptimal management and outcomes.In this review, we explore the applicability of MALDI imaging in sarcoma research, showcasing its value in understanding this highly heterogeneous and challenging rare cancer. We summarize all MALDI imaging studies in sarcoma to date, highlight their impact on key research fields, including molecular signatures, cancer heterogeneity, and drug studies. We address specific challenges encountered when employing MALDI imaging for sarcomas, and propose solutions, such as using formalin‐fixed paraffin‐embedded tissues, and multiplexed experiments, and considerations for multi‐site studies and digital data sharing practices.Through this review, we aim to spark collaboration between MALDI imaging researchers and clinical colleagues, to deploy the unique capabilities of MALDI imaging in the context of sarcoma.

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Visualizing the transdermal delivery of berberine loaded within chitosan microneedles using mass spectrometry imaging

Cui,

Geng,

Guo

et al. 2024

Anal Bioanal Chem

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Monitoring dexamethasone skin biodistribution with ex vivo MALDI-TOF mass spectrometry imaging and confocal Raman microscopy

Cited by 6 publications

References 40 publications

Optical Methods for Non-Invasive Determination of Skin Penetration: Current Trends, Advances, Possibilities, Prospects, and Translation into In Vivo Human Studies

Optical Methods for Non-Invasive Determination of Skin Penetration: Current Trends, Advances, Possibilities, Prospects, and Translation into In Vivo Human Studies

Advancing rare cancer research by MALDI mass spectrometry imaging: Applications, challenges, and future perspectives in sarcoma

Visualizing the transdermal delivery of berberine loaded within chitosan microneedles using mass spectrometry imaging

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