Monitoring DNA damage in indigenous blue mussels (Mytilus edulis) sampled from coastal sites in Denmark

Rank, Jette; Jensen, Klara; Jespersen, Per Homann

doi:10.1016/j.mrgentox.2005.04.008

Cited by 59 publications

(30 citation statements)

References 22 publications

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“…Nonetheless, we are not able to detect any genotoxic effect with both parameters (% DNA in tail and TME) measured with comet assay after exposure of mussels to contaminated Dessau sediment. In mussels, DNA damage is often measured in hemocytes (e.g., Binelli et al 2007;Riva et al 2007), although gills have been shown to detect genotoxic responses with greater sensitivity (Rank et al 2005). Indeed, at the beginning of our study, background DNA damage in D. polymorpha and D. bugensis was approximately 12% and 28% DNA in the tail, respectively, which is already relatively high.…”

Section: Discussionmentioning

confidence: 80%

Comparison of Bioaccumulation and Biomarker Responses in Dreissena polymorpha and D. bugensis After Exposure to Resuspended Sediments

Schäfer

Hamer

Treursić

et al. 2012

Arch Environ Contam Toxicol

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The zebra mussel Dreissena polymorpha is widely used as sentinel organism for the assessment of environmental contamination in freshwater environments. However, in the River Rhine (Germany), the D. polymorpha population is declining, whereas the closely related quagga mussel D. bugensis is found in high numbers at some sites. In the present laboratory study, D. polymorpha and D. bugensis were exposed to resuspended native sediments for ≤2 weeks. Wet sediments (<63 μm, 100 mg l(-1) dry weight) were used as surrogate suspended particulate matter to mimic one of the mussels' main uptake route for chemicals. The sediments were sampled in (1) the River Elbe in Dessau, a site known to be highly polluted with, e.g., organochlorine (OC) pesticides and (2) at a relatively unpolluted site in Havelberg in the River Havel, one of the Elbe's tributaries. Chemical analysis of persistent OC compounds (seven polychlorinated biphenyls [PCBs], DDT and its metabolites (DDX), hexachlorocylohexanes [HCHs], and hexachlorobenzene [HCB]) in soft tissue of mussels showed significantly greater values of PCBs 101, 118, 153, 138, 180, the sum of seven PCBs, and p,p'-DDD in D. bugensis compared with D. polymorpha. Fourteen days of exposure to Dessau sediment increased the concentration of p,p'-DDE and p,p'-DDD, as well as the sum of DDX, in both species compared with Havelberg sediment. Interspecific differences were less pronounced when regarding chemical concentrations with lipid content instead of dry-weight of tissue because D. bugensis had greater levels of total lipid than D. polymorpha. DNA damage in gills, as measured with the comet assay, was greater in D. bugensis compared with D. polymorpha. Simultaneously, the content of heat-shock protein (hsp70) in gills was greater in D. polymorpha than in D. bugensis. DNA damage and hsp70 were not induced by exposure time or sediment type. This study shows that D. bugensis and D. polymorpha may differ in their bioaccumulation potential of OC pesticides as well as their levels of DNA damage and hsp70. Therefore, more investigations are needed before quagga mussel can be used as alternative test organism for the zebra mussel.

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Section: Discussionmentioning

confidence: 80%

Comparison of Bioaccumulation and Biomarker Responses in Dreissena polymorpha and D. bugensis After Exposure to Resuspended Sediments

Schäfer

Hamer

Treursić

et al. 2012

Arch Environ Contam Toxicol

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“…The genotoxicity assessment in marine and freshwater invertebrates using the assay has been reviewed (Cotelle and Ferard 1999;Lee and Steinert 2003;Mitchelmore and Chipman 1998a). Cells from hemolymph, embryos, gills, digestive glands, and coelomocytes from mussels (Mytilus edulis; Rank et al 2005), zebra mussel (Dreissena polymorpha), clams (Mya arenaria), and polychaetes (Nereis virens) have been used for ecogenotoxicity studies using the Comet assay. DNA damage has also been assessed in earthworms (Salagovic et al 1996;Rajaguru et al 2003) and fruit fly, Drosophila, (Bilbao et al 2002;Mukhopadhyay et al 2004).…”

Section: Invertebratesmentioning

confidence: 99%

“…These sentinel species are adversely affected by pollution in the water bodies and thus provide the potential for environmental biomonitoring. The Comet assay in mussels can be used to detect a reduction in water quality caused by chemical pollution (Frenzilli et al 2001;Jha et al 2005;Rank et al 2005;Steinert et al 1998 Large et al 2002) and oil spills with petroleum hydrocarbons (Hamouten et al 2002). However, the damage returned to normal levels after continued exposure to high dose (20 ppb-exposed diet) of B[a]P for 14 days.…”

Section: Comet Assay In Musselsmentioning

confidence: 99%

Comet assay: a reliable tool for the assessment of DNA damage in different models

2008

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New chemicals are being added each year to the existing burden of toxic substances in the environment. This has led to increased pollution of ecosystems as well as deterioration of the air, water, and soil quality. Excessive agricultural and industrial activities adversely affect biodiversity, threatening the survival of species in a particular habitat as well as posing disease risks to humans. Some of the chemicals, e.g., pesticides and heavy metals, may be genotoxic to the sentinel species and/or to non-target species, causing deleterious effects in somatic or germ cells. Test systems which help in hazard prediction and risk assessment are important to assess the genotoxic potential of chemicals before their release into the environment or commercial use as well as DNA damage in flora and fauna affected by contaminated/polluted habitats. The Comet assay has been widely accepted as a simple, sensitive, and rapid tool for assessing DNA damage and repair in individual eukaryotic as well as some prokaryotic cells, and has increasingly found application in diverse fields ranging from genetic toxicology to human epidemiology. This review is an attempt to comprehensively encase the use of Comet assay in different models from bacteria to man, employing diverse cell types to assess the DNA-damaging potential of chemicals and/or environmental conditions. Sentinel species are the first to be affected by adverse changes in their environment. Determination of DNA damage using the Comet assay in these indicator organisms would thus provide information about the genotoxic potential of their habitat at an early stage. This would allow for intervention strategies to be implemented for prevention or reduction of deleterious health effects in the sentinel species as well as in humans.

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“…Whilst the body of evidence documenting these effects in vertebrates is high, there has been less attention given to the invertebrate species that occupy key ecological niches in marine habitats and for which genetic damage might have great potential for harm. The increasingly widespread aquatic distribution of chemicals with genotoxic potential has meant that the measurement of genotoxicity in the marine environment is fast becoming an area of great concern [3][4][5][6][7]. Sediments have long been recognised as a sink for organic contaminants such as polycyclic aromatic hydrocarbons (PAHs), which by virtue of their hydrophobic nature can strongly adsorb onto sediments affecting the benthic community inhabiting them.…”

Section: Introductionmentioning

confidence: 99%

Genotoxic damage in polychaetes: A study of species and cell-type sensitivities

Lewis

Galloway

2008

Mutation Research/Genetic Toxicology and Environmental Mutagene

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The marine environment is becoming increasingly contaminated by environmental pollutants with the potential to damage DNA, with marine sediments acting as a sink for many of these contaminants. Understanding genotoxic responses in sediment dwelling marine organisms, such as polychaetes, is therefore of increasing importance. This study is an exploration of species specific and cell-specific differences in cell sensitivities to DNA damaging agents in polychaete worms, aimed at increasing fundamental knowledge of their responses to genotoxic damage. The sensitivities of coelomocytes from three polychaetes species of high ecological relevance; the lugworm Arenicola marina, the harbour ragworm Nereis diversicolor and the king ragworm Nereis virens to genotoxic damage are compared, and differences in sensitivities of their different coelomic cell types determined using the comet assay. Arenicola marina was found to be the most sensitive to genotoxic damage from the direct acting mutagen methyl methanesulfonate (MMS), and showed dose dependent responses to MMS and the polycyclic aromatic hydrocarbon benzo(a)pyrene. Significant differences in sensitivity of the different types of 2 coelomocyte were also measured. Eleocytes were more sensitive to DNA damage than amoebocytes in both Nereis virens and Nereis diversicolor. Arenicola marina spermatozoa showed significant DNA damage following in vitro exposure to MMS, but were less sensitive to DNA damage than coelomocytes. This investigation has clearly demonstrated that different cell types within the same species and different species within the Polychaeta show significantly different responses to genotoxic insult. These findings are discussed in terms of the relationship between cell function and sensitivity and their implications for the use of polychaetes in environmental genotoxicity studies.

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Monitoring DNA damage in indigenous blue mussels (Mytilus edulis) sampled from coastal sites in Denmark

Cited by 59 publications

References 22 publications

Comparison of Bioaccumulation and Biomarker Responses in Dreissena polymorpha and D. bugensis After Exposure to Resuspended Sediments

Comparison of Bioaccumulation and Biomarker Responses in Dreissena polymorpha and D. bugensis After Exposure to Resuspended Sediments

Comet assay: a reliable tool for the assessment of DNA damage in different models

Genotoxic damage in polychaetes: A study of species and cell-type sensitivities

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