Monitoring Growth and Antibiotic Susceptibility of Escherichia coli with Photoluminescence Emitting Semiconductor Biochips

Nazemi, Elnaz; Hassen, Walid.; Frost, Éric; Dubowski, Jan J.

doi:10.1016/j.protcy.2017.04.104

Cited by 1 publication

(3 citation statements)

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“…Previously, we observed that the growth of E. coli on GaAs or Au surfaces required a minimum of 10 5 CFU/mL [44], while testing the sensitivity of E. coli strains to antibiotics with a photoluminescence-based biosensor was not possible for bacterial concentrations under 2 x 10 8 CFU/mL [24]. It has been suggested that the toxic effects of to Ab that neutralize viruses versus Ab that react with viruses but do not neutralize them [45].…”

Section: Discussionmentioning

confidence: 99%

“…Photoluminescence-based biosensors that usually employ GaAs surfaces have been used for detection of some bacteria [19][20][21][22][23][24] allowing quantification down to 10 3 colony forming units of bacteria (CFU) per ml and even lower with chemotaxis [25].…”

Section: Introductionmentioning

confidence: 99%

“…Photoluminescence-based biosensors have also been employed to determine antibiotic resistance of E. coli, but only with relatively low limits of detection [24]. The response of these biosensors depends on the efficiency of capturing target biomolecules with targetspecific ligands [26], such as antibodies [8,17], aptamers [27], antimicrobial peptides [28], lectins [15], phages [16] or molecular imprints [29] as opposed to non-specific binding to Au surface after coating with poly-lysine [18].…”

Section: Introductionmentioning

confidence: 99%

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Binding strategies for capturing and growing Escherichia coli on surfaces of biosensing devices

Choinière

Frost

Dubowski

2019

Talanta

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Antibiotic resistant bacteria have become a threat to world health. An advanced method of detection, based on the matrix assisted laser desorption ionisation time-of-flight mass spectroscopy can identify bacteria relatively rapidly, but it is not suitable to measure bacterial antibiotic resistance. Biosensors may be able to detect resistance by monitoring growth after capture on sensor surfaces but this option has not been addressed adequately. We have evaluated the growth of Escherichia coli after capture in 96 well microplates and observed that growth/capture efficiency was relatively similar for antibody-based techniques, but non-specific capture varied considerably. We confirm that neutravidin binds E. coli non-specifically, which limited its use with biotinylated antibodies or aptamers. Centrifugation enhanced bacterial growth/capture considerably, indicating that procedures enhancing the interaction between bacteria and surface-bound antibody have the potential to improve growth efficiency. Capture and growth required larger numbers of bacteria than capture and detection on biosensor surfaces. Previously, we reported that the minimum concentration of live E. coli required for initiating growth on a GaAs/AlGaAs biosensor was ~ 10 5 CFU/mL [Nazemi et al., Talanta 178 (2018) 69-77], and we speculated that this could be related to the poisonous effect of Ga-and As-ions released during dark corrosion of the biosensor, however in the present report we observed that the same minimum concentration of E. coli was required for growth in an ELISA plate. Thus, we argue that this limitation was related rather to bacterial inhibition by the capture antibodies. Indeed, antibodies at titres designed to capture bacteria inhibited bacterial growth when the bacteria were added to growth medium at titres less than 10 5 CFU/mL, indicating that antibodies may be responsible for the higher limits of sensitivity due to their potential to restrict bacterial growth. However, we did not observe E. coli release after 6 h following the capture indicating that these bacteria did not degrade antibodies.

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Section: Discussionmentioning

confidence: 99%