Monitoring healing of accidental laser burns of the macula using optical coherence tomography

Gadde, Santosh Gopi Krishna; Reddy, Nikitha Gurram; Sridharan, Akhila; Jayadev, Chaitra; Vinekar, Anand; Poornachandra, B

doi:10.1080/08164622.2021.1878860

Cited by 1 publication

(1 citation statement)

References 11 publications

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“…Comparison of multiple lasered regions within the same eye further confirmed the absence of interference between different lasered areas; no variability in laser outcome was noted, whether or not a strong or a mild DC laser was used in the adjacent lasered region (Figure 4, C and D, and Supplemental Figure 5). Qualitative analysis of SD-OCT images suggested increased thickness of the inner retina, likely caused by edematous changes immediately after the laser injury -as suggested previously (32) -and by retinal remodeling at later time points (33). OPL and HCC layers were visible for all 12 weeks after 1% DC laser (Figure 4, F-K) but were not visible after 1.5% DC laser injury (Figure 4, K-P) or 2% and 3% DC (Supplemental Figure 6, A-D), suggesting that DC laser higher than 1% disrupted INL organization, even though its thickness was maintained.…”

Section: Resultssupporting

confidence: 75%

A versatile laser-induced porcine model of outer retinal and choroidal degeneration for preclinical testing

Barone¹,

Amaral²,

Bunea³

et al. 2023

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Over 30 million people worldwide suffer from untreatable vision loss and blindness associated with childhood-onset and age-related eye diseases caused by photoreceptor (PR), retinal pigment epithelium (RPE), and choriocapillaris (CC) degeneration. Recent work suggests that RPE-based cell therapy may slow down vision loss in late stages of age-related macular degeneration (AMD), a polygenic disease induced by RPE atrophy. However, accelerated development of effective cell therapies is hampered by the lack of large-animal models that allow testing safety and efficacy of clinical doses covering the human macula (20 mm 2 ). We developed a versatile pig model to mimic different types and stages of retinal degeneration. Using an adjustable power micropulse laser, we generated varying degrees of RPE, PR, and CC damage and confirmed the damage by longitudinal analysis of clinically relevant outcomes, including analyses by adaptive optics and optical coherence tomography/angiography, along with automated image analysis. By imparting a tunable yet targeted damage to the porcine CC and visual streak — with a structure similar to the human macula — this model is optimal for testing cell and gene therapies for outer retinal diseases including AMD, retinitis pigmentosa, Stargardt, and choroideremia. The amenability of this model to clinically relevant imaging outcomes will facilitate faster translation to patients.

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Section: Resultssupporting

confidence: 75%