Monitoring intracellular pH using a hemicyanine-based ratiometric fluorescent probe

Xue, Xiao-Lei; Wang, Yan; Chen, Shaojin; Wang, Kunpeng; Niu, Shuyan; Zong, Qian‐Shou; Jiang, Y.; Hu, Zhiqiang

doi:10.1016/j.saa.2022.121778

Cited by 20 publications

(5 citation statements)

References 37 publications

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“…pH often had an impact on the fluorescence detection of probes. − To ensure that S-DCM-NIR (835) could recognize Cys at physiological pH (6.0–8.0), the response to Cys at different pH values was also investigated. As shown in Figure S5, the fluorescence of S-DCM-NIR (835) was independent of pH changes.…”

Section: Resultsmentioning

confidence: 99%

A Near-Infrared Fluorescent Dye with Tunable Emission Wavelength and Stokes Shift as a High-Sensitivity Cysteine Nanoprobe for Monitoring Ischemic Stroke

Wang,

Li,

et al. 2024

ACS Nano

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Sulfur-substituted dicyanomethylene-4H-chromene (DCM) derivatives based on the intramolecular charge transfer (ICT) mechanism were designed as near-infrared (NIR) fluorescent dyes. Using the Knoevenagel condensation method, the S-DCM-OH (835) fluorescence dye was synthesized, which had an emission wavelength exceeding 800 nm and 220 nm of a Stokes shift. Compared to commercial ICG, S-DCM-OH (835) was not only synchronized in emission wavelength but also far superior in Stokes shifts. These advantages made the design of S-DCM-NIR (835) based on this dye potentially valuable for biological applications. Based on this chemical structure, a fluorescent S-DCM-NIR (835) nanoprobe with a mean diameter of 17.69 nm was fabricated as the NIR imaging nanoprobe. Results showed that the nanoprobe maintained the high-specificity identification of cysteine (Cys) via the Michael addition reaction, with the detection limitation of 0.11 μM endogenous Cys. More importantly, in an ischemic stroke mouse model, the S-DCM-NIR (835) nanoprobe could monitor the Cys concentration change at stroke lesion due to the disruption of Cys metabolism under the ischemic stroke condition. Such a S-DCM-NIR (835) nanoprobe could not only differentiate the severity of the ischemic stroke using response time but also quantify the concentration of Cys in real-time in vivo.

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Section: Resultsmentioning

confidence: 99%

A Near-Infrared Fluorescent Dye with Tunable Emission Wavelength and Stokes Shift as a High-Sensitivity Cysteine Nanoprobe for Monitoring Ischemic Stroke

Wang,

Li,

et al. 2024

ACS Nano

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“…Hu et al designed a hemicyanine-based ratiometric FL probe (probe 1) with dual-emission properties that can be used to monitor changes in intracellular pH and elucidate the role of pH in many physiological processes. 336 The FL intensity of probe 1 at 769 nm dramatically increased with increasing pH, while that at 736 nm gradually decreased. This suggested that when the hydroxyl group in probe 1 protonates with the increase of pH value, the donor−acceptor effect in probe 1 is significantly enhanced.…”

Section: Ratiometric Optical Imagingmentioning

confidence: 94%

“…Previous studies have shown that the acid–base homeostasis in vivo controls the immune response, and abnormal pH can cause homeostasis dysregulation and lead to the occurrence of diseases. − Therefore, intracellular pH plays a crucial role in regulating cellular behavior and many important physiological and pathological processes. − pH responsiveness has practical significance in the design of ratiometric probes. Hu et al designed a hemicyanine-based ratiometric FL probe (probe 1) with dual-emission properties that can be used to monitor changes in intracellular pH and elucidate the role of pH in many physiological processes . The FL intensity of probe 1 at 769 nm dramatically increased with increasing pH, while that at 736 nm gradually decreased.…”

Section: Ratiometric Optical Imagingmentioning

confidence: 99%

Activatable Probes for Ratiometric Imaging of Endogenous BiomarkersIn Vivo

Fu,

Yang,

Wang

et al. 2024

ACS Nano

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Dynamic variations in the concentration and abnormal distribution of endogenous biomarkers are strongly associated with multiple physiological and pathological states. Therefore, it is crucial to design imaging systems capable of realtime detection of dynamic changes in biomarkers for the accurate diagnosis and effective treatment of diseases. Recently, ratiometric imaging has emerged as a widely used technique for sensing and imaging of biomarkers due to its advantage of circumventing the limitations inherent to conventional intensity-dependent signal readout methods while also providing built-in self-calibration for signal correction. Here, the recent progress of ratiometric probes and their applications in sensing and imaging of biomarkers are outlined. Ratiometric probes are classified according to their imaging mechanisms, and ratiometric photoacoustic imaging, ratiometric optical imaging including photoluminescence imaging and self-luminescence imaging, ratiometric magnetic resonance imaging, and dual-modal ratiometric imaging are discussed. The applications of ratiometric probes in the sensing and imaging of biomarkers such as pH, reactive oxygen species (ROS), reactive nitrogen species (RNS), glutathione (GSH), gas molecules, enzymes, metal ions, and hypoxia are discussed in detail. Additionally, this Review presents an overview of challenges faced in this field along with future research directions.

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“…One way to combat such factors ensuring reliability is to exploit the "ratiometric" approach, where the variations in two or more excitations or emissions values are measured to detect pH. Owing to its inherent reliability and precision, various ratiometric sensing probes have been developed to monitor pH in cellular quantification [105][106][107][108]. Xin Zhan and colleagues developed a fluorogenic sensing system by developing FR-TPP pH sensitive units (Figure 5) to trace mitophagy process [70].…”

Section: Absorption/visual-based Ph Sensorsmentioning

confidence: 99%

pH Monitoring in Biological Environment: An Overview of Conventional to Cutting-Edge Sensing Platforms

Akhtar¹,

Nawaz²,

Liu³

et al. 2023

Preprint

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pH is considered one of the paramount factors in bodily functions, because most of the cellular tasks exclusively rely on precise pH values. The regulation of pH is a necessary feature of the intracellular atmosphere and can be established as a strong indicator to judge a physiological abnormality in most of the cases. In this context, the current techniques for pH sensing provide us with the futuristic insight to further design therapeutic and diagnostic tools. Thus, pH-sensing (electrochemically and optically) is rapidly evolving toward exciting new applications and expanding researchers’ interests in many chemical contexts, especially in biomedical applications. The adaptation of cutting-edge technology is subsequently producing the modest form of these biosensors as wearable devices, which are providing us the opportunity to target the real-time collection of vital parameters, including pH for improved healthcare systems. The motif of this review is to provide an insight of trending tech-based systems employed in real time or in-vivo pH responsive monitoring. Herein, we briefly go through the pH regulation in the human body to help the beginners and scientific community with quick background knowledge, recent advances in the field, and pH detection in cancerous environments. In the end, we summarize our review by providing an outlook; challenges that need to be addressed and prospective integration of various pH in vivo platforms with modern electronics that can open new avenues of cutting-edge techniques for disease diagnostics and prevention.

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Monitoring intracellular pH using a hemicyanine-based ratiometric fluorescent probe

Cited by 20 publications

References 37 publications

A Near-Infrared Fluorescent Dye with Tunable Emission Wavelength and Stokes Shift as a High-Sensitivity Cysteine Nanoprobe for Monitoring Ischemic Stroke

A Near-Infrared Fluorescent Dye with Tunable Emission Wavelength and Stokes Shift as a High-Sensitivity Cysteine Nanoprobe for Monitoring Ischemic Stroke

Activatable Probes for Ratiometric Imaging of Endogenous BiomarkersIn Vivo

pH Monitoring in Biological Environment: An Overview of Conventional to Cutting-Edge Sensing Platforms

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