Monitoring Kidney Microanatomy Changes During Ischemia-Reperfusion Process Using Texture Analysis of OCT Images

Abstract

kidney ischemia-reperfusion (I/R) accounts for the majority of acute kidney injury cases, whose consequences are commonly encountered after kidney transplantation. Optical coherence tomography (OCT) has been applied to image changes in kidney microanatomy and microcirculation. In this paper, we demonstrate a quantitative method for monitoring kidney status during ischemia-reperfusion process using texture properties of OCT images. This approach employs skewness to measure the distribution of en face OCT image intensities at different depths, thus allowing differentiating ischemia-reperfusion status of kidney. The skewness analysis based on quantitative intensity shows promise for monitoring kidney status during ischemia-reperfusion, and the potential for evaluating the viability of transplant kidney.

Fully automated analysis of OCT imaging of human kidneys for prediction of post-transplant function

“…The LDKT group, and the DDKT-SCS SCD and ECD subgroups all experienced an increase in density between pre-implantation and postreperfusion scans. This is consistent with prior studies demonstrating a dramatic reduction in swelling of ischemic PCTs (which would present as an increase to total lumen area) following reperfusion [13,15]. In contrast to all other groups, the HMP group experienced a reduction in density following reperfusion, suggesting either some dissipation of the artificial dilation or induction of swelling.…”

“…These images reveal the microanatomy of the proximal convoluted tubules (PCTs), which comprise the majority of the superficial kidney cortex. Swelling of the epithelium of the PCTs is evident in OCT images and may be considered a symptom of ischemic insult [15,16]. Conversely, dilation of the tubular lumen is similarly evident in OCT and may be considered a symptom of pre-existing pathology or acute tubular injury (ATI) [17][18][19].…”

Optical coherence tomography and computer-aided diagnosis of a murine model of chronic kidney disease

“…1). [34][35][36][37] This system uses a swept-source laser operating at 1310-nm center wavelength with 100-nm bandwidth. The axial and lateral resolutions of the system are 12 and 6 μm, respectively.…”

Monitoring kidney optical properties during cold storage preservation with spatial frequency domain imaging

“…15,[29][30][31] Optical coherence tomography (OCT) has been used to assess structural changes to proximal convoluted tubules and glomeruli. [32][33][34][35][36] Multiphoton microscopy has been used in rodent studies to probe a number of fluorescent markers for structural and functional changes in kidneys at micron-level resolution. [37][38][39][40][41][42] During studies that involve human patients, imaging is performed after grafting and reperfusion of the kidney.…”