Monitoring monkeypox virus in saliva and air samples in Spain: a cross-sectional study

Correlation between monkeypox viral load and infectious virus in clinical specimens

“…Moreover, a recent study has also demonstrated infectious MPXV (assessed by cell culture positivity) in the saliva of infected individuals. [10] Collectively, these findings further highlight the multi-site, systemic nature of the recent mpox outbreak.…”

“…Our data further validate recent findings showing that samples with a higher MPXV viral load are more likely to demonstrate infectivity in cell culture. [ 8 , 10 , 11 ] For example, a recent study from Israel observed that clinical samples with a Ct value of ≥35 rarely grew in viral culture. [8] Similarly, Hernaez et al, showed that growth in viral culture was more likely in samples with Ct values < 26.…”

Human Monkeypox: Oral Implications and Recommendations for Oral Screening and Infection Control in Dental Practice

“…Sexual contact has been documented in about 90% of cases [35]. However, it is not yet clear whether the virus is sexually transmitted or spreads through direct contact with skin lesions or via body fluids such as saliva and respiratory secretions [36], specifically via droplets and contaminated surfaces [37], and via infectious aerosols, with an infected person during sexual activity [38,39].…”

“…In addition, MPXV remains infectious in aerosols for several hours [38], as also found in animal models [66], so although aerosolization is not the main route of transmission, it can be considered an important route of transmission in dental settings, further increasing the risk of occupational exposure for dental personnel and cross-infections in dental settings [26].…”

Transmission and detection of monkeypox virus in saliva (part II): Implications for sequential monitoring of viral load

“…Overall, the current evidence indicates that MPXV transmission through oral saliva might be a viable and recognized route, especially in the current 2022 outbreak of disease. 10 All sexually active men who have sex with men should be informed about the presence of MPXV on the skin, in the throat, in the anus, and in body fluids including oral saliva in case of infection. However, current evidence is not robust; the sample size is a major limitation.…”