Monitoring NAD(P)H by an ultrasensitive fluorescent probe to reveal reductive stress induced by natural antioxidants in HepG2 cells under hypoxia

Abstract

An ultrasensitive fluorescent probe for monitoring NAD(P)H and revealing reductive stress induced by natural antioxidants in HepG2 cells under hypoxia.

A bioorthogonal time-resolved luminogenic probe for metabolic labelling and imaging of glycans

“…S21 †). 48 Next, the sensing of Tb-1 towards TCO was measured in a physiological environment with the pH ranging from 8.0 to 5.0 by monitoring the Tb 3+ luminescence (Fig. 2e).…”

“…Calculation of the limit of detection (LOD). LOD was calculated using the formula, 48 LOD = 3δ/k. Here, δ represents the standard deviation of the fluorescence intensity of 3 blank solutions, and k represents the slope between fluorescence intensity and concentration.…”

Measuring ROS and redox markers in plant cells

“…Probes are typically based on a recognition unit that takes advantage of chemical reduction by NAD(P)H by linking compounds such as quinone, resazurin quinolinium and pyridine to fluorophores such as fluorescein, rhodamine and rhodol (see Sun et al 151 for a recent review). Whilst many of these probes have been established and tested in mammalian systems, often studying cancer, 152 there has been very little application to plants, potentially due to issues with cell wall and tissue permeability. A major limitation to current probes is their reliance on chemical reduction by NAD(P)H, leading to potential toxicity as cellular NAD(P)H is consumed.…”

Confining enzyme clusters in supramolecular nanoreactors enhances cofactor-dependent cascade for chiral alcohol synthesis

“…TCF-MQ is a small and sensitive probe to monitor NADPH, but did not respond to NADP + (Fig. S6) 49 . The negative control which contained the puri ed P22-SP-BmGDH-SsCR, 10 μM TCF-MQ, and 100 μM NADP + did not show any uorescence (Fig.…”