Monitoring of Aflatoxin M1 in milk using a novel electrochemical aptasensor based on reduced graphene oxide and gold nanoparticles

Ahmadi, Seyedeh Fatemeh; Hojjatoleslamy, Mohammad; Kiani, Hossein; Molavi, Hossein

doi:10.1016/j.foodchem.2021.131321

Cited by 42 publications

(6 citation statements)

References 38 publications

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“…This method of detection is precise, quick, and economical and can be used for on-site detection of aflatoxin in milk samples [ 75 , 76 ]. Another aptamer sensor-based technique has been recently used to detect aflatoxin M1 in the range 0.0005–0.8 μg/L, using a complex of graphene oxide with gold nano-particles (AuNPs) fabricated with a pencil graphite electrode (PGE) [ 77 ].…”

Section: Quantification/detection Of Aflatoxinmentioning

confidence: 99%

Aflatoxin Contamination, Its Impact and Management Strategies: An Updated Review

Shabeer

Asad

Jamal

et al. 2022

Toxins

124

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Aflatoxin, a type of mycotoxin, is mostly produced by Aspergillus flavus and Aspergillus parasiticus. It is responsible for the loss of billions of dollars to the world economy, by contaminating different crops such as cotton, groundnut, maize, and chilies, and causing immense effects on the health of humans and animals. More than eighteen different types of aflatoxins have been reported to date, and among them, aflatoxins B1, B2, G1, and G2 are the most prevalent and lethal. Early detection of fungal infection plays a key role in the control of aflatoxin contamination. Therefore, different methods, including culture, chromatographic techniques, and molecular assays, are used to determine aflatoxin contamination in crops and food products. Many countries have set a maximum limit of aflatoxin contamination (2–20 ppb) in their food and agriculture commodities for human or animal consumption, and the use of different methods to combat this menace is essential. Fungal infection mostly takes place during the pre- and post-harvest stage of crops, and most of the methods to control aflatoxin are employed for the latter phase. Studies have shown that if correct measures are adopted during the crop development phase, aflatoxin contamination can be reduced by a significant level. Currently, the use of bio-pesticides is the intervention employed in many countries, whereby atoxigenic strains competitively reduce the burden of toxigenic strains in the field, thereby helping to mitigate this problem. This updated review on aflatoxins sheds light on the sources of contamination, and the on occurrence, impact, detection techniques, and management strategies, with a special emphasis on bio-pesticides to control aflatoxins.

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Section: Quantification/detection Of Aflatoxinmentioning

confidence: 99%

Aflatoxin Contamination, Its Impact and Management Strategies: An Updated Review

Shabeer

Asad

Jamal

et al. 2022

Toxins

124

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“…Many methods have been used for the determination of AFB1, including thin-layer chromatography (TLC) [12,13], high-performance liquid chromatography (HPLC) [14][15][16][17], enzyme-linked immunosorbent assay (ELISA) [18][19][20][21], surfaces enhanced Raman scattering [22] and uorescence method [23]. However, all these methods have some disadvantages, like complicated pre-treatments, long analysis times, high analysis costs, bulky equipment, specialized operators, and the use of poor environmentally friendly substances [10,24]. Therefore, it is an important and imperative task to develop a simpler and e cient methodology for monitoring AFB1 in agricultural products [10].…”

Section: Introductionmentioning

confidence: 99%

Voltammetric determination of Aflatoxin B1 using a bismuth layer-gold nanoparticles modified glassy carbon electrode

Franco-Guzmán,

Castañeda-Ovando,

Mendoza-Huizar

et al. 2024

Preprint

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This work describes the development of an electroanalytical methodology for the direct determination of Aflatoxin B1 using a surface-modified vitreous carbon electrode with a bismuth film and gold nanoparticles. Cyclic voltammetry was used to determine the electrochemical behavior of Aflatoxin B1 on the working electrode. A well-defined cathodic wave was observed in the voltammogram which is associated with the direct reduction of Aflatoxin B1, so this signal was selected as the analytical response. To maximize this response, Square Wave Voltammetry was selected as the quantitative technique; then, pulse frequency, pulse amplitude, and potential step were optimized using a Box-Behnken surface design. A calibration curve was constructed in an interval of concentrations from 1 to 200 ngL− 1 with different Aflatoxin B1 standards; with the proper statistical analysis, it was found a linear range of 37.31 to 100 ngL− 1, with a limit of detection of 5.71 ng L− 1, and a sensitivity of 0.033 µA ngL− 1. A relative standard deviation percentage of 4.28 was obtained in the repeatability study. Finally, the proposed methodology was successfully used to analyze commercial milk samples.

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“…Multiple detection techniques are available for the qualitative or quantitative detection of AFM 1 in food systems. , Chromatographic methods based on large instruments have the advantage of accurate quantification, but they are often cumbersome and do not meet the need for rapid real-time detection . Immunoassays are simple, sensitive assays and rely on specific recognition between antibodies and antigens .…”

Section: Introductionmentioning

confidence: 99%

Screening of a High-Affinity Aptamer for Aflatoxin M₁ and Development of Its Colorimetric Aptasensor

Wei

Mahmood

et al. 2023

J. Agric. Food Chem.

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Aflatoxin M1 (AFM1), a secondary metabolite of Aspergillus spp., is highly toxic and widely present in food matrices. Therefore, the detection of AFM1 is of great importance for the protection of food safety. In this study, a five-segment sequence was designed as the initial library. Graphene oxide-SELEX (GO-SELEX) was applied to screen AFM1. After seven rounds of repeated screening, affinity and specificity assays showed that aptamer 9 was the best candidate for AFM1. The dissociation constant (K d) of aptamer 9 was 109.10 ± 6.02 nM. To verify the efficiency and sensitivity aptamer for the detection of AFM1, a colorimetric sensor based on the aptamer was constructed. The biosensor showed good linearity in the range of AFM1 concentration of 0.5–500.0 ng/mL with a detection limit of 0.50 ng/mL. This colorimetric method was successfully used for the detection of AFM1 in milk powder samples. Its detection recovery was 92.8–105.2%. This study was conducted to provide a reference for the detection of AFM1 in food.

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Monitoring of Aflatoxin M1 in milk using a novel electrochemical aptasensor based on reduced graphene oxide and gold nanoparticles

Cited by 42 publications

References 38 publications

Aflatoxin Contamination, Its Impact and Management Strategies: An Updated Review

Aflatoxin Contamination, Its Impact and Management Strategies: An Updated Review

Voltammetric determination of Aflatoxin B1 using a bismuth layer-gold nanoparticles modified glassy carbon electrode

Screening of a High-Affinity Aptamer for Aflatoxin M₁ and Development of Its Colorimetric Aptasensor

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Monitoring of Aflatoxin M1 in milk using a novel electrochemical aptasensor based on reduced graphene oxide and gold nanoparticles

Cited by 42 publications

References 38 publications

Aflatoxin Contamination, Its Impact and Management Strategies: An Updated Review

Aflatoxin Contamination, Its Impact and Management Strategies: An Updated Review

Voltammetric determination of Aflatoxin B1 using a bismuth layer-gold nanoparticles modified glassy carbon electrode

Screening of a High-Affinity Aptamer for Aflatoxin M1 and Development of Its Colorimetric Aptasensor

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Screening of a High-Affinity Aptamer for Aflatoxin M₁ and Development of Its Colorimetric Aptasensor