Monitoring of cell growth in vitro using biochips packaged with indium tin oxide sensors

Moore, Eric; Rawley, Orla; Wood, Terri; Galvin, Paul

doi:10.1016/j.snb.2008.11.025

Cited by 41 publications

(21 citation statements)

References 20 publications

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“…Even combinations with other electrical methods that use thin-film electrodes can be easily achieved, such as in situ electroporation of cells [95], Quartz Crystal Microbalance (QCM) [44], Surface Plasmon Resonance (SPR) [68], that allow for multiparametric analysis of the cell culture. Substrate-integrated electrodes can either be arranged as interdigitated comb structures (IDEs) [31,71,92,5] or use a small working electrode (WE) in combination with a large counter electrode (CE) (Fig. 2d, middle panel), as is the case for ECIS (described in more detail below) and other non-commercialized setups [119,77,80,55,38].…”

Section: Overview Of Endothelial Barrier Function Assaysmentioning

confidence: 99%

Impedance analysis of GPCR-mediated changes in endothelial barrier function: overview and fundamental considerations for stable and reproducible measurements

Stolwijk

Matrougui

Renken

et al. 2014

Pflugers Arch - Eur J Physiol

104

116

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The past 20 years have seen significant growth in using impedance-based assays to understand the molecular underpinning of endothelial and epithelial barrier function in response to physiological agonists, pharmacological and toxicological compounds. Most studies on barrier function use G protein coupled receptor (GPCR) agonists which couple to fast and transient changes in barrier properties. The power of impedance based techniques such as Electric Cell-Substrate Impedance Sensing (ECIS) reside in its ability to detect minute changes in cell layer integrity label-free and in real-time ranging from seconds to days. We provide a comprehensive overview of the biophysical principles, applications and recent developments in impedance-based methodologies. Despite extensive application of impedance analysis in endothelial barrier research little attention has been paid to data analysis and critical experimental variables, which are both essential for signal stability and reproducibility. We describe the rationale behind common ECIS data presentation and interpretation and illustrate practical guidelines to improve signal intensity by adapting technical parameters such as electrode layout, monitoring frequency or parameter (resistance versus impedance magnitude). Moreover, we discuss the impact of experimental parameters, including cell source, liquid handling and agonist preparation on signal intensity and kinetics. Our discussions are supported by experimental data obtained from human microvascular endothelial cells challenged with three GPCR agonists, thrombin, histamine and Sphingosine-1-Phosphate.

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Section: Overview Of Endothelial Barrier Function Assaysmentioning

confidence: 99%

Impedance analysis of GPCR-mediated changes in endothelial barrier function: overview and fundamental considerations for stable and reproducible measurements

Stolwijk

Matrougui

Renken

et al. 2014

Pflugers Arch - Eur J Physiol

104

116

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“…A great deal of attention is now being placed on inorganic nanomaterials because of their various potential applications in the production of electronic devices, sensors, biochips and energy storage media [3,4,5,6,7,8,9,10,11,12]. Inorganic materials have also been used to form superhydrophobic surfaces [13,14,15].…”

Section: Introductionmentioning

confidence: 99%

Wettability of Y2O3: A Relative Analysis of Thermally Oxidized, Reactively Sputtered and Template Assisted Nanostructured Coatings

et al. 2012

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The wettability of reactively sputtered Y2O3, thermally oxidized Y-Y2O3 and Cd-CdO template assisted Y2O3 coatings has been studied. The wettability of as-deposited Y2O3 coatings was determined by contact angle measurements. The water contact angles for reactively sputtered, thermally oxidized and template assisted Y2O3 nanostructured coatings were 99°, 117° and 155°, respectively. The average surface roughness values of reactively sputtered, thermally oxidized and template assisted Y2O3 coatings were determined by using atomic force microscopy and the corresponding values were 3, 11 and 180 nm, respectively. The low contact angle of the sputter deposited Y2O3 and thermally oxidized Y-Y2O3 coatings is attributed to a densely packed nano-grain like microstructure without any void space, leading to low surface roughness. A water droplet on such surfaces is mostly in contact with a solid surface relative to a void space, leading to a hydrophobic surface (low contact angle). Surface roughness is a crucial factor for the fabrication of a superhydrophobic surface. For Y2O3 coatings, the surface roughness was improved by depositing a thin film of Y2O3 on the Cd-CdO template (average roughness = 178 nm), which resulted in a contact angle greater than 150°. The work of adhesion of water was very high for the reactively sputtered Y2O3 (54 mJ/m2) and thermally oxidized Y-Y2O3 coatings (43 mJ/m2) compared to the Cd-CdO template assisted Y2O3 coating (7 mJ/m2).

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“…ITO is used as an impedance sensor that allows real time non-invasive in-vitro analysis of the physiological state of biological cells due to its conductive, biocompatible and transparent characteristics. Investigation verified that growth of cells on indium tin oxide sensor surface was comparable with growth of cells in tissue culture flasks (Moore et al, 2009). ITO electrodes can therefore be considered a valid alternative to gold electrodes as they can provide both optical and electrical information during the experiment (Choi et al, 2007).…”

Section: Introductionmentioning

confidence: 73%

Continuous Non-Destructive Monitoring of Cell Health Using Impedance Based Interdigitated Electrode Structured Sensors

Paschero¹,

McLoughlin²,

Moore³

et al. 2011

AIP Conference Proceedings

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Monitoring of cell growth in vitro using biochips packaged with indium tin oxide sensors

Cited by 41 publications

References 20 publications

Impedance analysis of GPCR-mediated changes in endothelial barrier function: overview and fundamental considerations for stable and reproducible measurements

Impedance analysis of GPCR-mediated changes in endothelial barrier function: overview and fundamental considerations for stable and reproducible measurements

Wettability of Y2O3: A Relative Analysis of Thermally Oxidized, Reactively Sputtered and Template Assisted Nanostructured Coatings

Continuous Non-Destructive Monitoring of Cell Health Using Impedance Based Interdigitated Electrode Structured Sensors

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