Monitoring of circulating amino acids in patients with pancreatic cancer and cancer cachexia using capillary electrophoresis and contactless conductivity detection

Tůma, Petr; Hložek, Tomáš; Kamišová, Jana; Gojda, Jan

doi:10.1002/elps.202100174

Cited by 13 publications

(10 citation statements)

References 51 publications

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“…All CE separations are performed in fused silica capillaries with id 25 µm, outer diameter 360 µm, total length 31.5 cm, and length to the C 4 D 18.0 cm (Polymicro Technologies, Phoenix, USA). Before first use, a new capillary is covered with the commercial product INST‐coating solution (Biotaq, http://www.biotaq.com, USA) for elimination of EOF [22] according to the procedure described in our previous article [23]. INST‐coating solution is also added to the input vial with the rinsing BGE solution in an amount of 1% v/v.…”

Section: Methodsmentioning

confidence: 99%

Monitoring of amoxicilline and ceftazidime in the microdialysate of diabetic foot and serum by capillary electrophoresis with contactless conductivity detection

et al. 2022

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Determination of the broad-spectrum antibiotics amoxicilline (AMX) and ceftazidime (CTZ) in blood serum and microdialysates of the subcutaneous tissue of the lower limbs is performed using CE with contactless conductivity detection (C 4 D). Baseline separation of AMX is achieved in 0.5 M acetic acid as the background electrolyte and separation of CTZ in 3.2 M acetic acid with addition of 13% v/v methanol. The CE-C 4 D determination is performed in a 25 µm capillary with suppression of the EOF using INST-coating on an effective length of 18 cm and the attained migration time is 4.2 min for AMX and 4.4 min for CTZ. The analysis was performed using 20 µl of serum and 15 µl of microdialysate, treated by the addition of acetonitrile in a ratio of 1/3 v/v and the sample is injected into the capillary using the large volume sample stacking technique. The LOQ attained in the microdialysate is 148 ng/ml for AMX and 339 ng/ml for CTZ, and in serum 143 ng/ml for AMX and 318 ng/ml for CTZ. The CE-C 4 D method is employed for monitoring the passage of AMX and CTZ from the blood circulatory system into the subcutaneous tissue at the sites of diabetic ulceration in patients suffering from diabetic foot syndrome and also for measuring the pharmacokinetics following intravenous application of bolus antibiotic doses.

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Section: Methodsmentioning

confidence: 99%

Monitoring of amoxicilline and ceftazidime in the microdialysate of diabetic foot and serum by capillary electrophoresis with contactless conductivity detection

et al. 2022

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“…, Thr (4), His (5), Cyn-mono-CBI (6), Glu (7), Gly (8), Tyr (9), Ala (10), Asp (11), Tau (12), Ala-Gln (13), Val (14), Met (15), nVal (16), Trp (17), Ile (18), Leu (19), Phe (20), Arg ( 21), Cyn-bis-CBI (22) and Lys (23). [81] with permission.…”

Section: Hplc Tandem Msmentioning

confidence: 99%

“…Alternatively, CE-C 4 D can also be used in combination with electromembrane extraction or microdialysis techniques for the direct detection of BCAAs in complex matrices. Tůma et al [19] utilize microdialysis techniques in conjunction with CE-C 4 D for the analysis of adipose tissue microdialysis metabolites (glucose, lactate, glycerol, and BCAAs). Adding acidified ACN to the sample on a 25 μm capillary coated with coating solution for the elimination of EOF (described by the same author before) improves the separation efficiency, and BCAAs more readily migrate from the sample zone.…”

Section: Ce-capacitively Coupled Contactless Conductivity Detectionmentioning

confidence: 99%

“…The separation efficiency was approximately 500 000 plates/m, and all resolutions were greater than 1. As a result of some minor changes to the above conditions, they successfully separated BCAAs, Ala, and Gln from the plasma of patients with pancreatic cancer and cancer cachexia syndrome using 13% v/v MeOH with 3.2 M AcOH as BGE in short capillaries of 18 cm [19].…”

Section: Approaches Without Derivatizationmentioning

confidence: 99%

“…However, to the best of our knowledge, there is currently no overview available that focuses on the analysis of BCAAs. BCAAs can be detected using various analytical methods, applying IEC [16], GC [17], HPLC [18], and CE [19]. It is important to note that the quantitative analysis of BCAAs is subject to two main challenges.…”

Section: Introductionmentioning

confidence: 99%

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Overview of chromatographic and electrophoretic methods for the determination of branched‐chain amino acids

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The significance of branched‐chain amino acids in diseases was clearly shown over the years. This review aims to describe the available techniques for their analytical determination. The article provides examples of the use of various analytical methods. The methods are divided into two categories: derivatization and non‐derivatization approaches. Separation is achieved through different chromatography or capillary electrophoresis techniques and can be combined with different detectors such as flame ionization, ultraviolet, fluorescence, and mass spectrometry. It compares the application of various derivatization reagents or detection as such for different detectors.

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Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

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2023

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This review article brings a comprehensive survey of developments and applications of high‐performance capillary and microchip electromigration methods (zone electrophoresis in a free solution or in sieving media, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) for analysis, micropreparation, and physicochemical characterization of peptides in the period from 2021 up to ca. the middle of 2023. Progress in the study of electromigration properties of peptides and various aspects of their analysis, such as sample preparation, adsorption suppression, electroosmotic flow regulation, and detection, are presented. New developments in the particular capillary electromigration methods are demonstrated, and several types of their applications are reported. They cover qualitative and quantitative analysis of synthetic or isolated peptides and determination of peptides in complex biomatrices, peptide profiling of biofluids and tissues, and monitoring of chemical and enzymatic reactions and physicochemical changes of peptides. They include also amino acid and sequence analysis of peptides, peptide mapping of proteins, separation of stereoisomers of peptides, and their chiral analyses. In addition, micropreparative separations and physicochemical characterization of peptides and their interactions with other (bio)molecules by the above CE methods are described.

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Monitoring of circulating amino acids in patients with pancreatic cancer and cancer cachexia using capillary electrophoresis and contactless conductivity detection

Cited by 13 publications

References 51 publications

Monitoring of amoxicilline and ceftazidime in the microdialysate of diabetic foot and serum by capillary electrophoresis with contactless conductivity detection

Monitoring of amoxicilline and ceftazidime in the microdialysate of diabetic foot and serum by capillary electrophoresis with contactless conductivity detection

Overview of chromatographic and electrophoretic methods for the determination of branched‐chain amino acids

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

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