Monitoring of contamination sources of Listeria monocytogenes in a poultry slaughterhouse

Schäfer, Daniela Fernanda; Steffens, Juliana; Barbosa, Juliana; Zeni, Jamile; Paroul, Natália; Valduga, Eunice; Junges, Alexander; Backes, Geciane Toniazzo; Cansian, Rogério Luís

doi:10.1016/j.lwt.2017.08.024

Cited by 37 publications

(29 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…From last few decades to present, studies on the presence of L. monocytogenes in poultry meat continue to be important in the worldwide. Unlikely to our findings, in many other studies a high-value prevalence was recorded, like Schafer et al [17] , who detected L. monocytogenes in 8.64-44.19% of chicken meat samples; Rahmat et al [18] , in…”

Section: Discussioncontrasting

confidence: 99%

Organik Tavuk Etlerinden İzole Edilen Listeria monocytogenes İzolatlarının Serotip ve Antibiyotik Direnç Profilinin Belirlenmesi

Gücükoğlu¹,

Çadırcı²,

Gülel³

et al. 2020

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

In this study, 240 organic chicken pieces (80 thighs, 80 wings, 80 skinless-breast meat) were analyzed for the presence of Listeria monocytogenes. Within the framework of the analysis findings; L. monocytogenes was detected in 60 (25%) of all 240 collected samples. In particular, L. monocytogenes was detected in 24 (30%) of 80 thigh samples, 20 (25%) of 80 wing samples and 16 (20%) of 80 skinless-breast meat samples. Serotyping distribution of 96 L. monocytogenes isolates determined as 71.8% serotype 1/2a, 21.9% serotype 1/2b, 4.2% serotype 4b and 2.1% serotype 1/2c. According to antibiotic resistance profile, 26 isolates (27%) were found to be resistant to ampicillin. The other isolates were found to be resistant to meropenem, tetracycline, sulfamethoxazole/trimethoprim, penicillin G, amoxicillin/clavulanic acid, vancomycin, oxytetracycline, erythromycin and chloramphenicol as 23 (23.9%), 14 (14.5%), 13 (13.5%), 12 (12.5%), 9 (9.3%), 7 (7.2%), 5 (5.2%), 4 (4.1%) and 3 (3.1%) respectively. Multiple antibiotic resistance profiles were determined in 12 of L. monocytogenes isolates. The findings of this study are thought to be unique data for serotyping studies that will help in revealing the epidemiology of L. monocytogenes in organic poultry meat, enterprises operating in food sector and diagnosis and treatment of listeriosis.

show abstract

Section: Discussioncontrasting

confidence: 99%

Organik Tavuk Etlerinden İzole Edilen Listeria monocytogenes İzolatlarının Serotip ve Antibiyotik Direnç Profilinin Belirlenmesi

Gücükoğlu¹,

Çadırcı²,

Gülel³

et al. 2020

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

show abstract

“…While it is true that, in practical terms, "non-contaminated 25-g units" may not provide sufficient confidence about the "contamination-free" status of the larger sample they represent (carcass, thigh, wings or breast), still the fact that Model 2 described the observed incidence data better than Model 1 consistently for the three pathogens, may suggest that "clean patches" occur more frequently in some chicken parts (breast, carcass) than others (wings, thigh), which probably arises from contamination patterns during processing and handling. These results are consistent for L. monocytogenes with those reported by Schäfer et al (2017) where higher prevalence levels were detected in thigh than in breast chicken samples. Nevertheless, this assumption can be tested in the future by increasing the representativeness of the sample, either by taking more 25-g units from the same chicken or by using surface swabbing methods.…”

Section: Adequacy Of Zero-inflated Binomial (Zib) Regression Modelssupporting

confidence: 92%

“…Poultry meat has also been implicated as a vehicle in the transmission of L. monocytogenes (Carpentier and Cerf, 2011;Alonso-Hernando et al, 2012) given its ability to grow at refrigeration temperatures in raw chicken meat (Sahu et al, 2017). The presence of L. monocytogenes has been reported in chicken breast (8.64%) and chicken thigh (44.19%) in poultry slaughter plants (Schäfer et al, 2017). Similar levels were found by Elmali et al (2015) in broiler wing meat samples (45%) highlighting seasonal differences in prevalence levels.…”

Section: Introductionmentioning

confidence: 89%

“…cross-contamination between flocks, animal breeding, faecal contamination etc.) and further transmitted to the poultry chain during slaughterhouse operations, distribution, retail marketing, preparation and consumption (Dookeran et al, 2012;Schäfer et al, 2017;Wang et al, 2018). Management of hazards transmitted to humans by consumption of chicken meat is therefore of major health and economic significance.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Zero-inflated binomial regressions for modelling low prevalence of pathogens in chicken meat as affected by sampling site

Hernández

Rodrı́guez-Lázaro

Valero

et al. 2018

Microbial Risk Analysis

View full text Add to dashboard Cite

Contamination of raw poultry meat with foodborne pathogens could occur because of improper handling at primary production and slaughterhouse levels. Low microbial prevalence data often consists of a high amount of non-detections (zero positives), so a flexible framework is required to characterise the underlying microbial distribution and conduct reliable inferential statistics. Thus, the objective of this work was to evaluate the performance of zero-inflated binomial (ZIB) regression models to describe the effects of sampling site (carcass, thigh, breast, wings) on the measured incidences of Salmonella, Listeria monocytogenes and Staphylococcus aureus on chicken meat. For each pathogen, four regression models based on the zero-inflated binomial ZIB (p, w 0) distribution were fitted to the presence/absence data with sampling site as covariate and random-effects due to sampling occasion either in the binomial probability (p) or in the extra-proportion of non-detections (w 0). For the three pathogens, the sampling site exerted a greater effect on w 0 than on p itself, with breast bearing the lowest prevalence estimates of Salmonella spp. (mean: 0.88%; 95% CI: 0.02-1.95%) and S. aureus (mean 1.48%; 95% CI: 0.01-4.00%). The fitting capacity of the models was further improved when random effects due to sampling occasion were placed in w 0 (deviances decreased from 146.7-156.7 to 140.2-140.6). This would imply that, theoretically, the variability in pathogens' occurrence from batch to batch mainly arises from the variability in non-contaminated zones. At any sampling site, the mean prevalence was estimated as 1.35 (95% CI: 0.15-2.70) for Salmonella, 2.11 (95% CI: 0.04-5.63) for L. monocytogenes and 2.36 (95% CI: 0.04-5.12) for S. aureus. Sampling performance analysis showed that wings were mostly suitable to detect Salmonella and S. aureus with higher probability (0.016 and 0.035 respectively), while for L. monocytogenes, sampling of thigh could be more effective (0.032).

show abstract

“…They were first described in 1926 by Murray and colleagues during an outbreak involving rabbits and guinea pigs in a laboratory in Cambridge (Great Britain). At that time, the bacteria were called Bacterium monocytogenes [ 177 , 178 ]. In the early 1980s, this pathogen became associated with human food poisonings [ 179 , 180 ].…”

Section: Listeriosismentioning

confidence: 99%

Campylobacteriosis, Salmonellosis, Yersiniosis, and Listeriosis as Zoonotic Foodborne Diseases: A Review

Chlebicz-Wójcik

Śliżewska

2018

IJERPH

373

227

View full text Add to dashboard Cite

Zoonoses are diseases transmitted from animals to humans, posing a great threat to the health and life of people all over the world. According to WHO estimations, 600 million cases of diseases caused by contaminated food were noted in 2010, including almost 350 million caused by pathogenic bacteria. Campylobacter, Salmonella, as well as Yersinia enterocolitica and Listeria monocytogenes may dwell in livestock (poultry, cattle, and swine) but are also found in wild animals, pets, fish, and rodents. Animals, often being asymptomatic carriers of pathogens, excrete them with faeces, thus delivering them to the environment. Therefore, pathogens may invade new individuals, as well as reside on vegetables and fruits. Pathogenic bacteria also penetrate food production areas and may remain there in the form of a biofilm covering the surfaces of machines and equipment. A common occurrence of microbes in food products, as well as their improper or careless processing, leads to common poisonings. Symptoms of foodborne infections may be mild, sometimes flu-like, but they also may be accompanied by severe complications, some even fatal. The aim of the paper is to summarize and provide information on campylobacteriosis, salmonellosis, yersiniosis, and listeriosis and the aetiological factors of those diseases, along with the general characteristics of pathogens, virulence factors, and reservoirs.

show abstract

Monitoring of contamination sources of Listeria monocytogenes in a poultry slaughterhouse

Cited by 37 publications

References 19 publications

Organik Tavuk Etlerinden İzole Edilen Listeria monocytogenes İzolatlarının Serotip ve Antibiyotik Direnç Profilinin Belirlenmesi

Organik Tavuk Etlerinden İzole Edilen Listeria monocytogenes İzolatlarının Serotip ve Antibiyotik Direnç Profilinin Belirlenmesi

Zero-inflated binomial regressions for modelling low prevalence of pathogens in chicken meat as affected by sampling site

Campylobacteriosis, Salmonellosis, Yersiniosis, and Listeriosis as Zoonotic Foodborne Diseases: A Review

Contact Info

Product

Resources

About