Monitoring of Endogenous Hydrogen Sulfide in Living Cells Using Surface‐Enhanced Raman Scattering

Abstract

Hydrogen sulfide (H2 S) has emerged as an important gasotransmitter in diverse physiological processes, although many aspects of its roles remain unclear, partly owing to a lack of robust analytical methods. Herein we report a novel surface-enhanced Raman scattering (SERS) nanosensor, 4-acetamidobenzenesulfonyl azide-functionalized gold nanoparticles (AuNPs/4-AA), for detecting the endogenous H2 S in living cells. The detection is accomplished with SERS spectrum changes of AuNPs/4-AA resulting from the reaction of H2 S with 4-AA on AuNPs. The SERS nanosensor exhibits high selectivity toward H2 S. Furthermore, AuNPs/4-AA responds to H2 S within 1 min with a 0.1 μM level of sensitivity. In particular, our SERS method can be utilized to monitor the endogenous H2 S generated in living glioma cells, demonstrating its great promise in studies of pathophysiological pathways involving H2 S.

Label‐Free Molecular Imaging of Biological Cells and Tissues by Linear and Nonlinear Raman Spectroscopic Approaches

“…[214] Thus judiciously designed capture molecules are necessary.I nt he case of intracellular CO detection, AuNPs were modified with palladacycles,and once CO bound to the Figure 8. [214] Thus judiciously designed capture molecules are necessary.I nt he case of intracellular CO detection, AuNPs were modified with palladacycles,and once CO bound to the Figure 8.…”

“…Aside from pH imaging within cells,the detection of small molecules such as CO and H 2 Sw ithin cells has gained attention as these molecules play significant roles in signaling pathways. [214] Thus judiciously designed capture molecules are necessary.I nt he case of intracellular CO detection, AuNPs were modified with palladacycles,and once CO bound to the Figure 8. SERS-based intracellularp Hsensing.…”

A silica-based SERS chip for rapid and ultrasensitive detection of fluoride ions triggered by a cyclic boronate ester cleavage reaction

“…One of the most prevalent ways is to utilise the molecular interactions of hydrogen bond, charge-transfer reaction and complexing coordination to amplify the Raman signals of prearranged molecules for the detection of Ramaninactive analytes. 30,31,32 Disadvantageously, these interactionbased approaches of Raman-inactive analytes are only performed in solution system, which would be easily affected by similar molecules, media or environments, leading to the unambiguous problem for the determination with false positive from the unavoidable aggregation of metal nanoparticles in real sample. 33,34 To the best of our knowledge, SERS nanosensors for the detection of Raman-inactive fluoride ions in real sample have not been previously reported for its extremely weak Raman signals and the unsolved problem of solution system in selectivity, sensitivity and reliability.…”

Intracellular and Cellular Detection by SERS‐Active Plasmonic Nanostructures

“…As an oninvasive strategy,S ERS nanosensors show many advantages,i ncludingr esistance to photobleaching and phototoxicity and to destruction of specimens in the detectiono fe ndogenousg aseous molecules.T ian and co-workersr eported the measuremento fe ndogenousH 2 Si n living cellsb yi ncorporating 4-acetamidobenzenesulfonyl azide (4-AA) as af unctional tago nt he AuNP surface. [26] Due to the reduction of azides by H 2 S, structure variations of 4-AA molecules would cause changes to the SERS spectrum;t hus giving rise to successful H 2 Sd etection. Long and co-workersd eveloped aC On anosensor constructed from palladacycle-assembled AuNPs.…”