Monitoring of environmental cancer initiators through hemoglobin adducts by a modified Edman degradation method

Törnqvist, Margareta; Mowrer, Jacques; Jensen, Søren Krogh; Ehrenberg, L.

doi:10.1016/0003-2697(86)90524-5

Cited by 325 publications

(159 citation statements)

References 21 publications

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“…Hagmar et al (2005)). Adducts to N-terminal valines were measured using the N-alkyl Edman method (Törnqvist et al, 1986) with modifications for measurement of AA adducts as described by Bergmark (1997). Globin, precipitated from lysed erythrocytes, was incubated with pentafluorophenyl isothiocyanate.…”

Section: Biobankmentioning

confidence: 99%

Associations between estimated acrylamide intakes, and hemoglobin AA adducts in a sample from the Malmö Diet and Cancer cohort

et al. 2007

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Objective: To examine the coherence of estimated intakes of acrylamide (AA) from foods, with hemoglobin (Hb) AA adduct levels, an objective marker of environmental AA exposure. Design: A cross-sectional study. Setting: The Malmö Diet and Cancer study, a large population-based prospective cohort (n ¼ 28 098) in the south of Sweden. Subjects: A sample of non-smoking (n ¼ 70) and smoking (n ¼ 72) women and men selected to obtain large variation in Hb AA adducts. Methods: Self-reported data on the usual consumption of foods were combined with published data on the AA content in Swedish foods. The Hb AA adduct levels were determined by a modified Edman degradation method. Linear regression and correlation analysis examined associations between estimated AA intakes, and Hb AA adducts. Results: In randomly selected individuals (n ¼ 40), the estimated median AA intake was 28 mg per day. In linear regression models, adjusting for sex, significant associations were seen in non-smokers between Hb AA adducts and estimated AA from foods (P ¼ 0.006). In smokers both AA from foods (P ¼ 0.006) and the calculated amount of tobacco consumed (P ¼ 0.003) were significantly associated with Hb AA adducts. Positive partial correlations between dietary AA estimates and Hb AA adducts were seen in smoking men (r ¼ 0.37) and women (r ¼ 0.59), and in non-smoking men (r ¼ 0.60), but not in non-smoking women. Conclusions: This study suggests that both diet and tobacco are important sources of the environmental AA exposure, although the lack of correlations in non-smoking women cast doubt on the validity of dietary AA intake estimates used in cancer epidemiology, or suggest that unrecognized factors may influence the internal dose measure of AA exposure.

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Section: Biobankmentioning

confidence: 99%

Associations between estimated acrylamide intakes, and hemoglobin AA adducts in a sample from the Malmö Diet and Cancer cohort

et al. 2007

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“…The stored deep-frozen erythrocyte fractions for the selected subjects (n = 142) were prepared for analysis of Hb adducts according to the N-alkyl Edman method described earlier ( [43] with modifications and materials for AA analysis according to [12]). …”

Section: Analysis Of Hb Adducts From Acrylamidementioning

confidence: 99%

Differences in hemoglobin adduct levels of acrylamide in the general population with respect to dietary intake, smoking habits and gender

Hagmar

Wirfält

Paulsson

et al. 2005

Mutation Research/Genetic Toxicology and Environmental Mutagene

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“…Thus, using the rat as a model, to generate a 50% increase over background levels of S-methylcysteine would require the animal to be administered about 20 mg nitrosodimethylamine/kg body weight (1). Exposure to stable isotope-labeled methylating agents has, however, satisfactorily been monitored (as there will naturally be no background adducts for these) through determinations of Hb Smethylcysteine (8) (12). The product is quantitated by GC-MS using globin modified by d4-ethylene oxide as internal standard.…”

Section: Hemoglobin Adductsmentioning

confidence: 99%

Identification of endogenous electrophiles by means of mass spectrometric determination of protein and DNA adducts.

Farmer

Bailey

Naylor

et al. 1993

Environ Health Perspect

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Monitoring exposure to alkylating agents may be achieved by quantitatively determining the adduct levels formed with nucleic acids and/or proteins. One of the most significant results arising from the application of this approach has been the discovery in control populations of "background" levels of alkylated nudeic acid bases or alkylated proteins, in particular hemoglobin (Hb). In the case of Hb, a wide variety ofsuch adducts have been detected and quantitated by mass spectrometric techniques, with methylated, 2-carboxyethylated, and 2-hydroxyethylated modifications being most abundant. Although the source of these alkylation products is unknown, both endogenous and exogenous sources may be proposed. We have recently confirmed the presence of the N-terminal hydroxyethylvaline adduct in control human Hb using tandem mass spectrometry (MS-MS) and have now established background levels using GC-MS in more than 70 samples. Smoking raises the levels of the adduct up to 10-fold and occupational exposure to ethylene oxide up to 300-fold.Background levels of alkylated nucleic acids may be studied by analysis ofN7-alkylated guanine or N3-alkylated adenine, which are excised from nucleic acids after their formation and are excreted in urine. Although the presence of some of these urinary constituents may be accounted for by their natural occurrence in RNA or diet, the endogenous or exogenous source ofothers is unknown. Quantitative methods using MS-MS have now been developed for five ofthe observed urinary alkylguanines [N7-methyl-, N2-methyl-, N2-dimethyl-, N7-(2-hydroxyethyl)-, and N2-ethylguanine]. A GC-MS method has also been developed to measure urinary thymine glycol as a possible monitor of oxidative DNA damage.

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Monitoring of environmental cancer initiators through hemoglobin adducts by a modified Edman degradation method

Cited by 325 publications

References 21 publications

Associations between estimated acrylamide intakes, and hemoglobin AA adducts in a sample from the Malmö Diet and Cancer cohort

Associations between estimated acrylamide intakes, and hemoglobin AA adducts in a sample from the Malmö Diet and Cancer cohort

Differences in hemoglobin adduct levels of acrylamide in the general population with respect to dietary intake, smoking habits and gender

Identification of endogenous electrophiles by means of mass spectrometric determination of protein and DNA adducts.

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