Monitoring of Food Spoilage Using Polydiacetylene‐ and Liposome‐Based Sensors

Weston, Max; Mazur, Federico; Chandrawati, Rona

doi:10.1002/9781119587422.ch5

Cited by 11 publications

(7 citation statements)

References 105 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…aptamers, antibodies) into the polymer network during synthesis. A broad and maturing body of research on PDA sensors is developing for medical, 12,13 environmental, 14 and food 15,16 related applications.…”

Section: Introductionmentioning

confidence: 99%

Polydiacetylene-based sensors for food applications

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Polydiacetylene-based sensors for food applications

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…Liposomes are self-assembling spherical vesicles composed of phospholipids which enclose an aqueous environment and have been extensively studied for biosensing applications. , For example, they have been used to encapsulate signal markers , or as recognition elements − for enzyme biomarker detection. Bui et al developed a highly sensitive enzyme-free plasmonic liposome immunoassay for the detection of several pathogens including Salmonella , Listeria , and Escherichia coli O157 ( E. coli O157) in water and food samples.…”

Section: Introductionmentioning

confidence: 99%

Rapid Detection of Listeriolysin O Toxin Based on a Nanoscale Liposome–Gold Nanoparticle Platform

Mazur

Tran

Kuchel

et al. 2020

ACS Appl. Nano Mater.

Self Cite

View full text Add to dashboard Cite

Listeria monocytogenes (L. monocytogenes) is an important foodborne pathogen responsible for foodborne listeriosis, a significant issue for high risk groups such as pregnant women. Currently available detection techniques for this bacterium, primarily nucleic acid-based methods, can achieve low detection limits; however, the assays are generally complex and lengthy. This pathogen can also be indirectly detected via the sensing of Listeriolysin O (LLO), a poreforming toxin secreted by L. monocytogenes. We present simple and rapid solution-and paper-based assays for the detection of poreforming toxin LLO. Liposomes are used as the natural recognition element in this assay because LLO acts primarily on lipid membranes. Pore-forming LLO triggers the release of cysteine from liposomes, which subsequently induces a rapid aggregation of gold nanoparticles present in the assay. The colorimetric response elicited by this aggregation enables LLO detection as low as 7.6 μg mL −1 for the solution-based assay, and limits of detection of 12.9 and 19.5 μg mL −1 LLO in PBS and spiked human serum, respectively, were obtained in 5 min for the paper-based assay. The assay does not require separation, enzyme-associated amplification, or washing steps, which are superior features in relation to point-of-care applications. The simplicity and rapidness of the nanoscale assay provides an opportunity for further development of portable sensors, specifically in resource-limited regions.

show abstract

“…Polydiacetylenes (PDAs) are a class of conjugated polymers exhibiting unique optical properties that render them ideal for the construction of colorimetric sensors to detect chemical and biological analytes. [1][2][3][4][5] They are synthesized from diacetylene (DA) monomers that undergo photopolymerization (by 254 nm UV light) via a 1,4-addition reaction to generate a polymeric backbone with alternating CQC and CRC bonds (ene-yne). Subjecting PDA to stimuli -such as light, heat, pH or a chemical/biological recognition event -can induce a perturbation in the polymer structure.…”

Section: Introductionmentioning

confidence: 99%

“…This orients the monomers favorably for photopolymerization and provides an excellent format for functionalization with recognition elements by insertion in or conjugation to the vesicle bilayer. [1][2][3][4][5][6][7][8][9] The conventional method for synthesis of PDA vesicles is the thin film hydration method (Bangham method) which was developed in 1965 for the production of liposomes from phospholipids. 10 Using this method, DA monomers are first dissolved in a suitable polar solvent, typically chloroform or ethanol.…”

Section: Introductionmentioning

confidence: 99%