Monitoring of intracellular localization of Hepatitis B virus P22 protein using Laser Scanning Confocal Microscopy and Airyscan

Deroubaix, Aurélie; Moahla, Bongani; Penny, Clement

doi:10.1002/jemt.23438

Cited by 8 publications

(3 citation statements)

References 16 publications

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“…There was increased retention of viral proteins in the secretory pathway, increased ER stress, and earlier and prolonged activation of UPR in cells transfected with subgenotype A1 [44]. When liver cells were transfected with replication competent plasmids of subgenotype A1, precore protein expression was affected by absence of core, which also influenced HBsAg expression, suggesting an interrelationship between precore proteins, HBcAg, and HBsAg [65]. Incorporating greater-than-genome-length subgenotype A1 sequences into recombinant adeno-associated viruses (AAVs), HBV replication was demonstrated in vitro in hepatoma cells and in mice [66].…”

Section: Molecular and Functional Characterization Of Hbv Subgenotype...mentioning

confidence: 98%

Hepatitis B Virus Research in South Africa

Maepa

Ely

Kramvis

et al. 2022

Viruses

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Despite being vaccine-preventable, hepatitis B virus (HBV) infection remains the seventh leading cause of mortality in the world. In South Africa (SA), over 1.9 million people are chronically infected with HBV, and 70% of all Black chronic carriers are infected with HBV subgenotype A1. The virus remains a significant burden on public health in SA despite the introduction of an infant immunization program implemented in 1995 and the availability of effective treatment for chronic HBV infection. In addition, the high prevalence of HIV infection amplifies HBV replication, predisposes patients to chronicity, and complicates management of the infection. HBV research has made significant progress leading to better understanding of HBV epidemiology and management challenges in the SA context. This has led to recent revision of the national HBV infection management guidelines. Research on developing new vaccines and therapies is underway and progress has been made with designing potentially curative gene therapies against HBV. This review summarizes research carried out in SA on HBV molecular biology, epidemiology, treatment, and vaccination strategies.

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Section: Molecular and Functional Characterization Of Hbv Subgenotype...mentioning

confidence: 98%

Hepatitis B Virus Research in South Africa

Maepa

Ely

Kramvis

et al. 2022

Viruses

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“…For size distribution estimation, bright-field microscopy images were captured of a 0.01% dilution of Zuper Black using the bright-field component of a ZEISS LSM 780 confocal laser scanning microscope with an alpha Plan-Apochromat 63×/1.40 NA Oil CorrM27 objective lens (Carl Zeiss AG, Oberkochen, Germany) [39].…”

Section: Methodsmentioning

confidence: 99%

On the attenuation of ultrasound by pure black tattoo ink

et al. 2021

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Black tattoo ink comprises hydrophobic carbon black nanoparticles. We hypothesized that black tattoo ink demonstrates transient dynamic activity in an ultrasound field. Brightness-mode sonography was performed on cylindrical receptacles of different bore diameters, filled with black tattoo ink, water, saline, or air, using pulsed ultrasound with center frequencies of 13 MHz and 5 MHz.The scattering from black ink itself lasted less than ten minutes. At 13-MHz sonication, a transient drop in sound speed was observed, as well as a transient lessening of scattering from distal phantom tissue. The linear acoustic attenuation coefficient of pure black ink was measured to be 0.15±0.01 dB cm −1 MHz −1 , equal to whole blood.Low-intensity ultrasonic tattoo removal would be of interest as an alternative to techniques that damage surrounding tissue.

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“…This technique, called laser scanning confocal microscopy (LSCM) [ 8 , 9 ], was originally developed for application in biology and medicine. Now, it is more and more widely used and has become an essential tool in biomedical experimental research [ 10 , 11 , 12 , 13 ]. Compared to conventional fluorescence microscopy, it overcomes the shortcomings of an out-of-focal plane-fluorescence imaging blur and focal plane glare and can improve the optical resolution of fluorescence microscopy.…”

Section: Introductionmentioning

confidence: 99%

Near-Infrared Light-Excited Quinolinium-Carbazole Small Molecule as Two-Photon Fluorescence Nucleic Acid Probe

Sun,

Wu,

Liu

et al. 2024

Molecules

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This article reports three new two-photon absorption (TPA) materials that are quinolinium-carbazole derivates. They are 3-(N-methyl-4-ethylquinolinium iodide)-9-ethylcarbazole (M4), 3-(N-methyl-4-ethylquinolinium iodide)-9-ethylcarbazole (H2), and 3-(N-methyl-4-ethylquinolinium iodide)-9-ethylcarbazole (H4). Their TPA cross-sections are 491, 515, and 512 GM, respectively. Under the excitation of near-infrared light, their fluorescence emission is about 650 nm. The compounds can stain nucleic acid DNA with the same level of nuclear localization as Hoechst 33342. Under continuous irradiation with a near-infrared laser, the three new compounds showed less fluorescence decay than DAPI, and the average fluorescence decay rates were 0.016%/s, 0.020%/s, and 0.023%/s. They are expected to become new two-photon fluorescent probes of nucleic acid DNA because of their excellent performance.

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Monitoring of intracellular localization of Hepatitis B virus P22 protein using Laser Scanning Confocal Microscopy and Airyscan

Cited by 8 publications

References 16 publications

Hepatitis B Virus Research in South Africa

Hepatitis B Virus Research in South Africa

On the attenuation of ultrasound by pure black tattoo ink

Near-Infrared Light-Excited Quinolinium-Carbazole Small Molecule as Two-Photon Fluorescence Nucleic Acid Probe

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