This paper presents: (a) results of gross primary production (GPP) 8-d estimated values using a light use efficiency model (LUE) in a non-irrigated rotating rapeseed crop in the upper Spanish plateau, and (b) inter-comparison results of observed GPP with those concurrently retrieved by MODIS. The rotation scheme over the four-year study comprised rapeseed, wheat, peas and rye. Rapeseed, peas and, in part, rye grew under well-watered conditions whereas wheat was dominated by drought. Input data for the LUE model were the fraction of PAR absorbed (FPAR) 8-d products supplied by MODIS (FPAR MODIS), in situ photosynthetic active radiation (PAR) measurements and a scalar f varying between 0 and 1, to take into account the reduction of the maximum PAR conversion efficiency (ε 0LUE) under limiting environmental conditions. In this study, f values were assumed to be dependent on air temperature (T) and the evaporative fraction which was considered a proxy of water availability. ε 0LUE , a key parameter in LUE models, which varied according to land use, was derived through the results of a linear regression fit between observed GPP and concurrent G APAR estimates defined as the product of PAR, FPAR MODIS and f. Overall, the LUE model provided satisfactory results, R 2 = 86.3%, significantly improving GPP MODIS estimates (GPP MODIS), R 2 = 71.8%. GPP MODIS uncertainties have primarily been attributed to differences in the f stress factor involved in its formulation (f MODIS) depending on vapour pressure deficit and T which did not fully describe the environmental stress conditions at the measuring site. Overall, ε 0LUE yielded 3.33 ± 0.10 g C MJ −1 although this varied depending on crop architecture, phenology and prevailing meteorological conditions. Crop-to-crop ε 0LUE ranged from 2.74 ± 0.17 to 3.95 ± 0.19 g C MJ −1 for peas and rye, respectively, yielding intermediate values for rapeseed and wheat, 2.92 ± 0.18 and 2.86 ± 0.23 g C MJ −1 , respectively. ε 0MODIS , derived from the linear fit of GPP versus GPP MODIS estimates, yielded 2.13 ± 0.10 g C MJ −1 and crop-to-crop ranged from 1.28 ± 0.17 to 2.41 ± 0.12 g C MJ −1 for wheat and rapeseed, respectively. The best linear fits corresponded to crops growing under well-watered conditions, rapeseed and peas, and the worst fits were for wheat, affected by drought. GPP annuals were 1680, 710, 730 and 1410 g C m −2 for rapeseed, wheat, peas and rye, respectively.