Monitoring of microhemodynamic changes during ex vivo xenogeneic liver perfusion using intravital microscopy

Abstract

Monitoring of microhemodynamic changes during ex vivo xenogeneic liver perfusion using intravital microscopycylate (Aspisol) and the platelet-ac-The combination showed a synergistic effect. After apheresis of preformed xenogeneic antibodies, the parameters measured were comparable with those seen in isogeneic experiments. Complement degradation with cobra venom factor revealed a minor improvement in perfusion. A rapid, extensive, and irreversible leukocyte accumulation in terminal portal vessels was observed in all xenogeneic experiments. Blood counts of the perfusate confirmed the early trapping of leukocytes and platelets in the xenoperfused liver, indicating nonimmunological, cellular involvement in this rejection process.

Literature update 1998, part 3

“…There were several additional reports on the use of intravital microscopy to observe the process of hyperacute rejection in the microcirculation [13–15]. These, and other studies [16] demonstrated the importance of complement activation during this type of rejection.…”

Release of pig leukocytes and reduced human NK cell recruitment during ex vivo perfusion of HLA‐E/human CD46 double‐transgenic pig limbs with human blood

“…Although there are major differences in terms of endothelial damage during short-term xenoperfusions reported in the literature, in part because different pig organs were employed,[47][48][49][50][51] and the exact contribution of NK cells is unknown, the expression of HLA-E might reduce endothelial damage by preventing NK cell activation. We cannot directly support this hypothesis with experimental data from our model, since the functional assays originally planned with circulating T A B L E 2 Phenotypic characteristics of the pig cell populations appearing during xenoperfusion…”