Monitoring of Progesterone in Captive Female False Killer Whales, Pseudorca crassidens

Atkinson, Shannon; Combelles, Catherine M.H.; Vincent, D.L.; Nachtigall, Paul E.; Pawloski, Jeffrey L.; Breese, Marlee

doi:10.1006/gcen.1999.7319

Cited by 62 publications

(52 citation statements)

References 17 publications

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“…However, the bulk of mammalian endocrine literature reports steroid hormone levels from blood (Temte 1991, Kjeld et al 1992, Atkinson et al 1999 and to a lesser degree urine (Robeck et al 1993), feces (Rolland et al 2005(Rolland et al , 2006, and saliva (Atkinson et al 1999, Hogg et al 2005). Other sample materials from which these hormones have been extracted and measured include milk (West et al 2000), muscle (Yoshioka et al 1994), cetacean exhalant or blow (Hogg et al 2005), bone, and ocular secretions (Atkinson et al 1999).…”

Section: Introductionmentioning

confidence: 99%

“…Other sample materials from which these hormones have been extracted and measured include milk (West et al 2000), muscle (Yoshioka et al 1994), cetacean exhalant or blow (Hogg et al 2005), bone, and ocular secretions (Atkinson et al 1999). Only a scant few studies have measured reproductive hormones from adipose tissue of any kind, let alone blubber (Hillbrand & Elsaesser 1983, Ha mu di ku wanda et al 1996, Mansour et al 2002, Kellar et al 2006, though one of these does include data from minke whales Balaenoptera acutorostrata, a species of baleen whale (Mansour et al 2002).…”

Section: Introductionmentioning

confidence: 99%

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Variation of bowhead whale progesterone concentrations across demographic groups and sample matrices

Kellar

Keliher²,

Trego³

et al. 2013

Endang. Species. Res.

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Bowhead whale Balaena mysticetus progesterone concentrations were measured in different sample matrices (serum, blubber, and urine) to investigate (1) concordance among sample type and (2) variation among life-history class. Samples were collected from subsistencehunted whales (n = 86) taken from 1999 to 2009. In general, irrespective of sample matrix, pregnant females had the highest concentrations by orders of magnitude, followed by mature animals of both sexes, and subadults had the lowest concentrations. Subadult males and females had similar progesterone concentrations in all sample matrices measured. When pregnant animals were included in our analyses, permuted regression models indicated a strong positive relationship between serum and blubber progesterone levels (r 2 = 0.894, p = 0.0002). When pregnant animals were not included, we found no significant relationship between serum and blubber levels (r 2 = 0.025, p = 0.224). These results suggest that progesterone concentrations are mirrored in these sample types over longer periods (i.e. on the order of weeks to months, time frame of reproductive changes) but not shorter periods (i.e. on the order of hours to days, time frame of daily fluctuations). This conclusion is consistent even for progesterone concentrations measured in females that had recently changed pregnancy states (either new mothers or newly pregnant animals), for which blubber progesterone levels seem to lag those in the serum. Finally, urine progesterone had statistically significant positive relationships with serum (r 2 = 0.136, p = 0.0460) and blubber progesterone (r 2 = 0.150, p = 0.0421). Our results suggest that progesterone concentrations first peak in the serum, then in the urine, and finally in the blubber.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Variation of bowhead whale progesterone concentrations across demographic groups and sample matrices

Kellar

Keliher²,

Trego³

et al. 2013

Endang. Species. Res.

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“…All extracted blubber samples will be assayed in duplicate using the validated assays. Various forms of this analytical validation have been conducted over the years on a variety of biological media in our Endocrine Laboratory (Seltmann et.al., 2012;Trumble et al, 2012;Villegas-Amtmann et al, 2012;Verrier et al, 2012;Atkinson et al 2011;Wang et al 2010;Myers et al, 2009;Villegas-Amtmann et al, 2009;Nilsson et al, 2008;Atkinson et al, 2008a;Petrauskas et al, 2008;Mashburn and Atkinson, 2008;Mellish et al, 2007;Mashburn and Atkinson, 2007;Greig et al, 2007;Mashburn and Atkinson, 2004;Oki and Atkinson, 2004;West et al, 2000;Atkinson et al, 1999).…”

Section: Report Documentation Pagementioning

confidence: 99%

Development and Validation of a New Technique for Detection of Stress and Pregnancy

Atkinson

2014

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Award Number: N00014-14-1-0425 https://www.sfos.uaf.edu LONG-TERM GOALSTo promote the use of endocrine techniques to advance our knowledge of the physiology of cetaceans and their responses to change in their environment. OBJECTIVESTwo objectives are being nested under the overall goal of developing techniques that can be used for the detection of stress and pregnancy in large whales.1)The first objective is to develop and conduct analytical and preliminary biological validations of pregnancy and stress hormones for large whales (humpback whales, blue whales, and possibly insular false killer whales).2) The second objective is to complete the biological validation using archived samples from whales with known life history or behaviors. APPROACHThe project has a two year approach in which the first year will serve as the proof of concept and the second year will complete the validation and add in life history and behavior data. Details of each year's activities are separated below.Year 1 -Commercially available radioimmunoassays (RIA) previously validated in other marine mammal species will be validated for corticosterone or cortisol and progesterone in humpback whale blubber. The validation will follow two forms, analytical and biological, described below. Analytical Validation:The analytical validation will be conducted using standard methods of parallelism, accuracy and metabolite identification using high-pressure liquid chromatography (HPLC). Briefly, pooled blubber extract from animals of known gender will be serially diluted 1:2 and run as samples, in duplicate, in the assay to determine displacement by the pool. Accuracy of the assay will be determined by spiking the pool at the appropriate dilution with known amounts of hormone then performing the assays. Mass of the pool will be calculated and subtracted from the mass measured

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“…Many of the studies cited here use more than one matrix sampled from the same animal to validate the accuracy of the matrices for measuring hormones (e.g., blood and saliva; blood and urine; and blood, saliva and ocular secretions) (ATKINSON et al, 1999;KJELD 2001;. We hope this review can provide not only basic information on cetacean hormone measurements but also guidance useful to marine mammal researchers for choosing the most suitable technique and biological matrices according to the species studied in the field.…”

Section: Discussionmentioning

confidence: 99%

“…Currently, certain species, such as the bottlenose dolphin (Tursiops spp.) (CORNELL et al, 1987;SCHROEDER;KELLER, 1989;OWEN, 1990), the killer whale (Orcinus orca) (DUFFIELD et al, 1995), the false killer whale (Pseudorca crassidens) (ATKINSON et al, 1999), belugas (ROBECK et al, 2005a) and the Pacific white-sided dolphin (Lagenorhynchus obliquidens) have been well studied (O'BRIEN et al, 2008;ROBECK et al, 2009). Baseline values for progesterone, testosterone and estradiol were determined for most of these species.…”

Section: Discussionmentioning

confidence: 99%

Uso de gordura subcutânea como matriz biológica na dosagem de esteroides sexuais em baleias jubarte (Megaptera novaeangliae)

Mello¹

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A pesquisa com baleias envolve a mobilização de muitas pessoas para que as coisas funcionem. A logística não é algo simples, tampouco as condições ambientais -muito sol, muito frio, muito balanço, muito trabalho, muito cansaço, muita concentração e muita baleia! Não me esquecerei dos inúmeros momentos em que colegas, parceiros, estagiários, voluntários, funcionários e amigos não mediram esforços para que os dados fossem colhidos da melhor maneira possível, MUITO OBRIGADA PESSOAL!!! Gostaria de agradecer aos meus pais pelo apoio desde sempre, por me darem condições e coragem para estudar o que gosto. Agradeço em especial à minha mãe, minha inspiração e grande incentivadora.Agradeço muitíssimo ao meu orientador e amigo Prof Cláudio Alvarenga de Oliveira por ter aceitado esse desafio de trabalhar com baleias. Agradeço a confiança em mim depositada e seu apoio sempre presente. Você foi um grande incentivador! Agradeço à Priscila Viau por me ensinar a técnica de Enzimaimunoensio empregada neste estudo Agradeço imensamente à FAPESP pela bolsa de estudo e também pelo financiamento do projeto. Agradeço a agilidade e profissionalismo da Fundação. Os recursos disponibilizados pela FAPESP foram cruciais à execução deste estudo.Agradeço ao Instituto Baleia (IBJ) Jubarte pelo apoio logístico nas colheitas de campo e na base de Caravelas. Agradeço especialmente Milton Marcondes por "comprar" a ideia desde o início e por sua amizade.Agradeço muitíssimo a Adriana Colosio, veterinária do IBJ pelas colheitas de campo e pela amizade, valeu paraguaia! Agradeço a todos os funcionários do IBJ, especialmente aos motoristas Sr. Vavá e espigão por dirigir horas e horas atrás de carcaça de baleia.Agradeço aos mestres e marinheiros presentes nas saídas de campo em Abrolhos, em especial Lecréu e Todynho.Agradeço à colega de profissão e parceira na maior parte das colheitas em Abrolhos Kátia Groch Agradeço muitíssimo às estagiárias do IBJ em 2011: Juliana Molás, Paula Coutinho, Bruna Buzzato e em especial à Geovanna Campos; aos estagiários de 2012: Arielle Bezerra, Isis Vuolo, Vinicius Melo e em especial Waltyane Bonfim; e aos estagiários de 2013: Yasmin Viana, Júlia Pierry e em especial à Cintia Brito e Fábio Mendes.Agradeço a Jorge Acevedo e equipe da Fundación CEQUA pela parceria de pesquisa, e pelo apoio nas colheitas feitas na Patagônia chilena.Agradeço a Nicholas Kellar por abrir as portas do laboratório do Southwest Fisheries Science Center em San Diego para que eu pudesse fazer treinamento laboratorial. Agradeço também a Marisa Trego por me ensinar as técnicas de extração e dosagem de hormônios da camada de gordura de cetáceos.Agradeço às secretárias do VRA Harumi, Thaís, e Roberta por toda ajuda nestes quatro anos de doutorado. Agradeço à Natacha Orteney pela ajuda nas extrações das amostrasAgradeço aos estagiários, funcionários, e ex-funcionários que trabalham com foto-identificação no Instituto Baleia Jubarte.Agradeço à Faculdade de Medicina Veterinária e Zootecnia não só pela disponibilidade de espaço físico, mas pelas muitas op...

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Monitoring of Progesterone in Captive Female False Killer Whales, Pseudorca crassidens

Cited by 62 publications

References 17 publications

Variation of bowhead whale progesterone concentrations across demographic groups and sample matrices

Variation of bowhead whale progesterone concentrations across demographic groups and sample matrices

Development and Validation of a New Technique for Detection of Stress and Pregnancy

Uso de gordura subcutânea como matriz biológica na dosagem de esteroides sexuais em baleias jubarte (Megaptera novaeangliae)

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