Monitoring of the Sensitivity of Magnaporthe grisea to Metominostrobin 2001-2003: No Emergence of Resistant Strains and No Mutations at Codon 143 or 129 of the Cytochrome b Gene

Araki, Yasuo; Sugihara, Minoru; Sawada, Haruko; Fujimoto, Hiroyuki; Masuko, Michio

doi:10.1584/jpestics.30.203

Cited by 15 publications

(9 citation statements)

References 6 publications

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“…For detection of strobilurin-resistant isolates, several reports indicated that the point mutation at codons 129 and 143 of the cyt b gene is applicable. 21,30) However, isolates of M. oryzae in Taiwan did not show a point mutation at codon 129 or 143 of the cyt b gene. Consequently, the mechanism of suspected low strobilurin sensitivity in isolates of M. oryzae was not caused by the known point mutations in the cyt b gene.…”

Section: Discussionmentioning

confidence: 95%

“…ABC transports are also closely related to multiple drug resistances. 21,42) Thus, the AOX and ABC transports might play an important role in reducing the damage of strobilurin fungicides in the less sensitive M. oryzae isolates from Taiwan. It warrants further investigation to confirm the mechanism of the resistance to strobilurins.…”

Section: Discussionmentioning

confidence: 99%

“…20) Many fungicides, including acid amides, aliphatic compounds, organo-phosphorus compounds, antibiotics, benzimidazoles, strobilurins, organo-sulphur compounds and heterocyclic compounds, are recommended for controlling rice blast. 21,22) Among these compounds, carpropamid of acid amides and tricyclazole of heterocyclic compounds are the most commonly used fungicides in Taiwan. 22) Based on the guidelines published by the Fungicides Resistance Action Committee (FRAC), the melanin biosynthesis inhibitors (MBIs) belonging to Code I could be separated into two groups based on their target enzyme: reductase inhibitors (MBI-R) that include tricyclazole and dehydratase inhibitors (MBI-D) that include carpropamid.…”

Section: Introductionmentioning

confidence: 99%

“…23,29) Two strobilurin fungicides, azoxystrobin and metominostrobin, were used to control rice blast in Japan. 21) Moreover, azoxystrobin and trifloxystrobin were used to control ryegrass disease caused by P. grisea, however, the two strobilurin fungicides lost its efficacy in controlling P. grisea. 30) In Taiwan, strobilurin fungicides are commonly used for controlling powdery or downy mildew of cucumber, downy mildew of crucifer, late blight of tomato and potato, rust disease of allium, anthracnose of fruits and other fungal diseases of fruits.…”

Section: Introductionmentioning

confidence: 99%

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Phylogenetic diversity and sensitivity to MBI and QoI fungicides of <i>Magnaporthe oryzae</i> in Taiwan

Hsieh

Chung²,

Chen³

2013

J. Pestic. Sci.

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Magnaporthe oryzae (Anamorph: Pyricularia oryzae), the causal agent of rice blast disease, is the major pathogen that reduces the yield of rice worldwide. Melanin biosynthesis inhibitors (MBIs) and strobilurins (QoIs) are common fungicides used to control the disease. One hundred and three M. oryzae isolates from different rice-production areas in Taiwan were evaluated for their molecular phylogeny and sensitivity to MBI and QoI fungicides. Molecular phylogenetic analysis showed that all M. oryzae isolates could be classified into nine genetic groups (A-I) based on the DNA polymorphism amplified by CNS1/MP primers. Predominant ones are groups A (72 isolates) and B (15 isolates). Fungicide tests showed that M. oryzae isolates were sensitive to MBIs (carpropamid and tricyclazole) and less sensitive to QoIs (kresoxim-methyl and azoxystrobin). Amplifying the cytochrome b (cyt b) gene revealed that isolates with low sensitivity to QoIs did not correspond with mutation at codon 129 or 143 in the cyt b gene.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Phylogenetic diversity and sensitivity to MBI and QoI fungicides of <i>Magnaporthe oryzae</i> in Taiwan

Hsieh

Chung²,

Chen³

2013

J. Pestic. Sci.

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“…Recently, M. oryzae strains resistant to azoxystrobin have been detected in the field in Japan (1,18,43), and decreased sensitivity was reported also in M. oryzae from perennial ryegrass in the United States, as a consequence of the mutation in the cytochrome b gene (24,40). On the other hand, attempts to obtain stable tricyclazole-resistant strains in laboratory were not successful, and strains with slightly decreased tricyclazole sensitivity showed lower fitness, reduced sporulation, and pathogenicity to rice (44).…”

Section: Discussionmentioning

confidence: 99%

Sensitivity of Nonexposed and Exposed Populations of Magnaporthe oryzae from Rice to Tricyclazole and Azoxystrobin

et al. 2014

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Kunova, A., Pizzatti, C., Bonaldi, M., and Cortesi, P. 2014. Sensitivity of nonexposed and exposed populations of Magnaporthe oryzae from rice to tricyclazole and azoxystrobin. Plant Dis. 98:512-518.Magnaporthe oryzae is the major pathogen of cultivated rice worldwide, which can cause substantial losses to rice production. Rice blast management is based predominantly on the application of fungicides; however, only a little is known about responses of pathogen populations to the most widely used fungicides. In this work, the baseline sensitivity of the Italian M. oryzae population to tricyclazole and azoxystrobin in terms of mycelium growth was determined, and the possible adaptation of the pathogen population after several years of repeated exposure to fungicide treatments was evaluated. All the analyzed strains demonstrated higher sensitivity and variability to azoxystrobin (concentration of fungicide causing 50% growth inhibition [ED 50 ] = 0.063 mg liter -1 ) than to tricyclazole (99.289 mg liter -1 ). After comparing two additional populations collected from fields repeatedly treated with fungicides to the baseline, no decrease in sensitivity toward these fungicides was observed and no resistant strains were detected. The shift of the pathogen sensitivity toward these fungicides has not occurred, although we observed slightly increased variance associated with ED 50 of azoxystrobin. Therefore, both azoxystrobin and tricyclazole can be used to manage rice blast in Italy but it will be important to continue monitoring M. oryzae population to early detect possible azoxystrobin resistance.

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Site‐directed mutagenesis of the cytochrome b gene and development of diagnostic methods for identifying QoI resistance of rice blast fungus

Wei

Katoh

Nishimura

et al. 2009

Pest Management Science

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Monitoring of the Sensitivity of Magnaporthe grisea to Metominostrobin 2001-2003: No Emergence of Resistant Strains and No Mutations at Codon 143 or 129 of the Cytochrome b Gene

Cited by 15 publications

References 6 publications

Phylogenetic diversity and sensitivity to MBI and QoI fungicides of <i>Magnaporthe oryzae</i> in Taiwan

Phylogenetic diversity and sensitivity to MBI and QoI fungicides of <i>Magnaporthe oryzae</i> in Taiwan

Sensitivity of Nonexposed and Exposed Populations of Magnaporthe oryzae from Rice to Tricyclazole and Azoxystrobin

Site‐directed mutagenesis of the cytochrome b gene and development of diagnostic methods for identifying QoI resistance of rice blast fungus

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