Monitoring of the Viscoelastic Behaviour of Bacterial Biofilms Exploiting an Accurate QCM System

Fort, Ada; Landi, Elia; Grasso, Anna Lo; Mugnaini, Marco; Panzardi, Enza; Vignoli, Valerio; Mahdizadeh, Fariba Fahmideh; Magnani, Agnese

doi:10.1109/memea54994.2022.9856431

Cited by 4 publications

(2 citation statements)

References 26 publications

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“…the resonance frequency, varies in a small frequency range in any measurement conditions therefore the ratio 𝑓 𝑐 𝑓 𝑠 ′ can be assumed to remain constant. In QCM measurement at 10 MHz the expected maximum shift is always limited to few kHz [4] [6], in practical cases for biosensors, the meaningful shifts are limited well below 1 kHz [24,25]. From (7), we have that considering the typical Q values of QCMs, the resonant frequency, i.e.…”

Section: A Theorymentioning

confidence: 99%

A Smart Low-Cost QCM-D Based on Lightweight Frequency Domain Processing

Carli,

Fort,

Micheletti

et al. 2024

IEEE Trans. Instrum. Meas.

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This work presents an accurate low-cost smart measurement system capable of simultaneously monitoring the QCM series resonant frequency and dissipation factor with high accuracy, optimally adapting its settings to the operating conditions. The designed system is based on a microcontroller, is fully programmable and it exploits an ad hoc signal processing technique. The performance of the proposed measurement system was assessed experimentally using a prototype coupled with the quartz via an antenna pair, allowing non-contact measurements. Experimental results were obtained under different measurement conditions. The results demonstrated the effectiveness of the system, which is able to guarantee high frequency accuracy even in the case of large mechanical loads, such as those that occur in in-liquid measurements. In liquid-loaded conditions, when working with transient response signals characterized by time constants as low as 60 μs, a resolution of 10 Hz is found.

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Section: A Theorymentioning

confidence: 99%

A Smart Low-Cost QCM-D Based on Lightweight Frequency Domain Processing

Carli,

Fort,

Micheletti

et al. 2024

IEEE Trans. Instrum. Meas.

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“…This allows for external factors to impact the resonant frequency and damping factor of the exposed crystal, which makes it possible to indirectly measure various physical quantities. QCM properties make it suitable for many applications in modern science such as biosensors [ 4 , 5 , 6 , 7 ], vapors and particulate matter sensors [ 8 , 9 , 10 ], systems for characterization of liquids [ 11 , 12 , 13 ], and aerospace [ 14 , 15 , 16 , 17 , 18 ]. Underlying most experiments are indirect measurements of mass changes and viscoelastic properties [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Precision Temperature Control System with Low EMI for Applications in Analyzing Thermal Properties of Highly Sensitive Piezoelectric Sensors

Nowocień

Wielgus

Mroczka

2022

Sensors

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A low electromagnetic interference (EMI), precision temperature control system for sensitive piezoelectric sensors stabilization and their thermal characteristics research was proposed. Quartz crystal microbalance (QCM) was chosen as the device to be tested. Recently, QCMs found use in many fields of study such as biology, chemistry, and aerospace. They often operate in harsh environments and are exposed to many external factors including temperature fluctuations, to which QCMs are highly susceptible. Such disturbances can cause undesirable resonant frequency shifts resulting in measurement errors that are difficult to eliminate. The proposed solution enables measurements of QCMs thermal characteristics, effectiveness evaluation of temperature compensation methods, and testing of the frequency stability. As a part of the developed solution, two independent temperature regulators were used: first to maintain the QCM crystal at desired temperature, and second to keep the QCM oscillator circuit at fixed temperature. The single regulator consists of a thermoelectric module (TEC) used for both heating and cooling. Two considered TEC driving methods were compared in terms of EMI and their impact on the QCM signal quality. The proposed system was examined for its temperature stabilization capability showing high stability of 11 mKp-p for one hour and the setpoint accuracy of ±15 mK in the full temperature range.

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Acoustic Fingerprinting and Nanoslip Dynamics of Biofilms

Jana,

Bhalla

2024

Adv Funct Materials

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It is reported that bacteria can generate nanomotion, but understanding the complex dynamics of bacterial colony gliding on solid interfaces has remained unresolved. Here, this work captures the real‐time development and gliding of bacterial biofilms on vibrating solids made of piezoelectric quartz. The gliding, characterized by liquid slips, is measured in form of frequency and dissipation changes of the vibrating solid. These vibrations enable the generation of distinct acoustic fingerprints (sound/ music) of the three phases of biofilm development: viscoelastic strengthening, biofilm growth and biofilm stability. In adition, the effect of extracellular matrix secretion on the rigidity of the film and its nanoslip in each of the distinct biofilm developmental phases is quantified. This work provides a real‐time, label‐free method of quantifying bacteria biofilm dynamics and paves the way for developing libraries of acoustic signatures of bacteria and their metabolic products.

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Monitoring of the Viscoelastic Behaviour of Bacterial Biofilms Exploiting an Accurate QCM System

Cited by 4 publications

References 26 publications

A Smart Low-Cost QCM-D Based on Lightweight Frequency Domain Processing

A Smart Low-Cost QCM-D Based on Lightweight Frequency Domain Processing

Precision Temperature Control System with Low EMI for Applications in Analyzing Thermal Properties of Highly Sensitive Piezoelectric Sensors

Acoustic Fingerprinting and Nanoslip Dynamics of Biofilms

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