Monitoring of volatiles: A technique for detection of soft rot (<i>Erwinia carotovora</i>) in potato tubers

Waterer, D.; Pritchard, M. K.

doi:10.1080/07060668409501578

Cited by 32 publications

(22 citation statements)

References 9 publications

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“…For this reason, they were not considered to be significant as potential marker volatiles for a soft-rot detection system, even though ethanol was previously identified as one of the most significant vapours when E. carotovora infection occurs. Concentrations were shown to rise by as much as 2000 times with infection (Waterer & Pritchard, 1984), whilst other alcohols such as propan-1-ol remain at relatively constant levels. Other volatiles are less well documented.…”

Section: Discussionmentioning

confidence: 99%

“…Preliminary work and that of other workers showed ethanol, methanol and acetaldehyde to be present with both infected and uninfected potato tubers (Waterer & Pritchard, 1984). For this reason, they were not considered to be significant as potential marker volatiles for a soft-rot detection system, even though ethanol was previously identified as one of the most significant vapours when E. carotovora infection occurs.…”

Section: Discussionmentioning

confidence: 99%

“…Many low molecular weight volatiles, especially alcohols, were evolved during infection (Waterer & Pritchard, 1984); for example, ethanol, methanol and butan-1-ol were found to increase rapidly as infection progressed (Varns & Glynn, 1979). However, these volatiles tend not to be disease specific and are also evolved when no infecting organisms are present (Maga, 1994).…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies of potato tubers infected by E. carotovora have identified a range of VOCs evolved during the progression of the disease (Varns & Glynn, 1979;Waterer & Pritchard, 1984;Maga, 1994) which might be useful indicators of the onset of infection. Many low molecular weight volatiles, especially alcohols, were evolved during infection (Waterer & Pritchard, 1984); for example, ethanol, methanol and butan-1-ol were found to increase rapidly as infection progressed (Varns & Glynn, 1979).…”

Section: Introductionmentioning

confidence: 99%

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Identification of volatiles generated by potato tubers (Solanum tuberosum CV: Maris Piper) infected by Erwinia carotovora, Bacillus polymyxa and Arthrobacter sp.

et al. 1999

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Bacteria were isolated from internal tissues of surface sterilized healthy tubers of Solanum tuberosum cv. Maris Piper (8 different isolates) and from tubers inoculated with Erwinia carotovora ssp. carotovora showing soft-rot symptoms (3 different isolates), and identified by fatty acid profiling. Bacillus polymyxa and an Arthrobacter sp. were isolated from both sources, E. carotovora only from the soft-rotted tubers. The volatile organic compounds (VOCs) generated by tubers inoculated with E. carotovora, B. polymyxa and the Arthrobacter sp. were identified. Inoculated tubers of cv. Maris Piper were incubated under controlled humidity (95% relative humidity) and temperature (10ЊC) to simulate typical storage conditions. B. polymyxa and Arthrobacter sp. did not cause symptoms, whilst E. carotovora caused limited soft-rot infections after 4 weeks at the low temperatures typically associated with potatoes in storage. The VOCs released to the headspace around these tubers were collected using an adsorbent system and analysed by Gas Chromatography-Mass Spectrometry (GC-MS). Twenty-two volatiles unique to E. carotovora infection of potato tubers were found, including 10 alkanes, four alkenes, two aldehydes, one sulphide, one ketone, one alcohol, one aromatic, one acid and one heterocyclic compound. B. polymyxa generated three unique volatiles: N,Ndimethylformamide, 1-pentadecene and 1-hexadecane. Only one volatile, 2,3-dihydrofuran, was unique to the Arthrobacter infection. Production of volatile nitrogen species from E. carotovora-infected tubers increased with time, whereas none were detected in the headspace above uninfected tubers. Further analysis using a modified GC-MS method established that ammonia, trimethylamine and several volatile sulphides were evolved from tubers infected by E. carotovora. No specific volatile was useful as a marker associated with any of the three bacterial species but in the case of E. carotovora-infected potato tubers a significant increase in the volume of compounds evolved was clearly observed. The results are discussed in relation to the use of sensors to detect VOCs evolved from infected tubers in order to provide an early warning system for the control of soft rot in potato stores.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification of volatiles generated by potato tubers (Solanum tuberosum CV: Maris Piper) infected by Erwinia carotovora, Bacillus polymyxa and Arthrobacter sp.

et al. 1999

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“…Therefore, there is a good correlation between the smell of potatoes and its degree of damage. The potato disease can be detected firstly by its volatile gas concentration and thus, the disease degree can be recognized [9][10][11][12][13][14][15]. Stinson et al proposed a healthy recognition ability of the disease in potato by an electronic nose [16]; however, the technology required further research; DE Lacy Costello et al designed an electronic nose system, which could detect diseased potatoes within healthy potatoes [17]; however, https://doi.org/10.1515/biol-2017-0044…”

Section: Introductionmentioning

confidence: 99%

Bacterial Infection Potato Tuber Soft Rot Disease Detection Based on Electronic Nose

Chang

et al. 2017

Open Life Sciences

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Soft rot is a severe bacterial disease of potatoes, and soft rot infection can cause significant economic losses during the storage period of potatoes. In this study, potato soft rot was selected as the research object, and a type of potato tuber soft rot disease early detection method based on the electronic nose technology was proposed. An optimized bionic electronic nose gas chamber and a scientific and reasonable sampling device were designed to detect a change in volatile substances of the infected soft rot disease of potato tuber. The infection of soft rot disease in potato tuber samples was detected and identified by using the RBF NN algorithm and SVM algorithm. The results revealed that the proposed bionic electronic nose system can be utilized for early detection of potato tuber soft rot disease. Through comparison and analysis, the recognition rate using the SVM algorithm reached up to 89.7%, and the results were superior to the RBF NN algorithm.

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The soft rot Erwinia

Charkowski

Plant-Associated Bacteria

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Monitoring of volatiles: A technique for detection of soft rot (Erwinia carotovora) in potato tubers

Cited by 32 publications

References 9 publications

Identification of volatiles generated by potato tubers (Solanum tuberosum CV: Maris Piper) infected by Erwinia carotovora, Bacillus polymyxa and Arthrobacter sp.

Identification of volatiles generated by potato tubers (Solanum tuberosum CV: Maris Piper) infected by Erwinia carotovora, Bacillus polymyxa and Arthrobacter sp.

Bacterial Infection Potato Tuber Soft Rot Disease Detection Based on Electronic Nose

The soft rot Erwinia

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