Monitoring periodontal disease status in smokers and nonsmokers using a gingival crevicular fluid matrix metalloproteinase‐8‐specific chair‐side test

Abstract

Persistently elevated GCF MMP-8 concentrations may indicate sites at risk, as well as patients with poor response to conventional periodontal treatment (e.g. SRP). MMP-8 testing may be useful as an adjunct to traditional periodontal diagnostic methods during the maintenance phase.

Asociación entre niveles de TNF-α en fluido crevicular gingival de dientes con periodontitis apical asintomática

“…En la actualidad ya existen algunos dispositivos de uso en clínica para determinar experimentalmente los niveles de estos biomarcadores, particularmente para la MMP-8. Al respecto se ha visto que las variaciones en los niveles de esta enzima pueden diferenciar sujetos sanos, de sujetos con gingivitis y periodontitis, como también evaluar la respuesta al tratamiento periodontal e identificar sujetos en riesgo de progresión (26)(27)(28) . De este modo, una herramienta no invasiva para detectar la presencia de inflamación periapical como el FCG sería de gran utilidad como complemento a la evaluación clínico-radiográfica de dientes con PAA, particularmente en aquellos casos en que la interpretación radiográfica sea incierta (29) .…”

Components of Host Response to Pathogenic Bacteria in Gingivitis

“…This finding suggests that host response during gingivitis might be heterogeneous and some of the inflammatory phenotypes could be more susceptible to periodontitits development . Accordingly, elevation of active MMP-8 has previously been associated with the conversion of gingivitis to periodontitis and established periodontitis (Mantyla et al, 2006). Despite MMP-13 has been more related to chronic periodontitis progression than severity of inflammation, it has previously been reported that MMP-13 also increase progressively from healthy subjects to periodontitis in GCF.…”

The Role of Immuno-Inflammatory Response in the Pathogenesis of Chronic Periodontitis and Development of Chair-Side Point of Care Diagnostics

“…These procedures, as well as GCF sampling, are technically demanding and the GCF volume can be very small (1-5 µl). Despite these apparent diagnostic and technical disadvantages, GCF is still considered as a candidate potential oral fluid for the development of adjunctive non-invasive chair-side point of-care diagnostic technology (Sorsa et al 1999;Sorsa et al 2006;Sorsa, Tjaderhane, and Salo 2004;Mantyla et al 2006;Mantyla et al 2003;Munjal et al 2007), especially because tissue destructive MMPs and their bioactive regulators can conveniently be measured by distinct catalytic and non-catalytic immunoassays from GCF ).…”

“…During a study period, it is likely that only a small number of sites with AL can be confirmed. Additionally, only a small group of periodontitis patients manifest multiple progressing sites (Chambers et al 1991;Mantyla et al 2006). In our previous study, we could not make a conclusion about the predictive value of MMP-8 testing .…”

“…Although periodontal clinical examination is necessary and cannot be substituted by any other means in periodontal diagnostics, biomarker testing could give relevant clinical adjunctive information about the individual's host response levels, although there is no known normal range at present. Selective antibodies for detection of active MMP-8 in oral fluids have been utilized as adjunctive diagnostic point-of-care/chair-side tests identifying sites susceptible for periodontitis progression and periodontitis affected patients Mantyla et al 2006;. We have recently i n v e s t i g a t e d l e v e l s o f G C F M M P -8 w i t h t w o d i f f e r e n t c h a i r -s i d e ( d e n t o A n a l y z e r b y dentognostics GmbH and MMP-8 specific chair-side dip-stick test) and two laboratory methods (immunofluorometric assay, IFMA, and commercial ELISA) .…”