Monitoring Potential‐Induced DNA Dehybridization Kinetics for Single Nucleotide Polymorphism Detection by using In Situ Surface Enhanced Raman Scattering

Kayran, Yasin Uğur; Cinar, Nergis; Jambrec, Daliborka; Schuhmann, Wolfgang

doi:10.1002/celc.201701220

Cited by 7 publications

(6 citation statements)

References 27 publications

(27 reference statements)

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“…They found a direct correlation between melting potential and thermal stability, where 9 mV shift in the melting potential corresponds to 1 °C change in melting temperature [52] . Schuhmann and coworkers employed potential ramps on dye‐labeled ssDNA layers to investigate the integrity of the ssDNA/mercaptohexanol (MCH) modified electrodes and focused on retaining the stability of the sensing layer [53] . By comparing dehybridization and desorption curves of ds‐ and ssDNA layers, respectively, they determined that a single dehybridization potential is sufficient to induce dsDNA dehybridization without desorbing the immobilized ssDNA from the electrode surface (Figure 8).…”

Section: It Takes Two To Tango: How the Interfacial Electric Field Ca...mentioning

confidence: 99%

DNA Electrostatics: From Theory to Application

Gębala

Jambrec

2022

ChemElectroChem

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Numerous DNA biosensor platforms developed in the last decades rely on DNA electrostatics as the basis for detection. However, the overwhelming number of theoretical studies and computational models of DNA electrostatics poses a barrier to leveraging our deeper mechanistic understanding in this area for the development of new technologies that will push the field towards more sensitive, quantitative, and reliable DNA‐based sensors. In this review, we will bridge the gap between the theory and applications of DNA charge and electrical surfaces to overcome this barrier. We will discuss key theories such as Manning's counterion condensation theory, the Poisson‐Boltzmann equation, and the Gouy‐Chapman model, and provide examples of technological applications that rely on these theories.

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Section: It Takes Two To Tango: How the Interfacial Electric Field Ca...mentioning

confidence: 99%

DNA Electrostatics: From Theory to Application

Gębala

Jambrec

2022

ChemElectroChem

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“…Additionally, in situ SERS measurements allow for the fast and efficient (on the timescale of minutes) detection of single nucleotide polymorphism based on potential‐assisted dehybridization of target dsDNA [77] …”

Section: Dna Rna and Conformational Changesmentioning

confidence: 99%

In Situ and Operando Techniques for Investigating Electron Transfer in Biological Systems

et al. 2020

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When bioelectrochemistry meets other analytical techniques for in situ experiments, new possibilities for understanding the nature of charge‐transfer reactions are provided. Despite progress in recent years in analytical instrumentation, a number of key issues remains for bioelectrochemistry, in terms of the mechanistic description of the surface reactions involved in biomolecules’ electron transfer. The main challenges of these techniques are the concomitant detection of electron transfer and molecular alteration as well as the development of suitable bioelectrodes. This review discusses the most recent and emerging in situ and operando electrochemical methods used to study biological systems such as redox proteins, nucleic acids, small biomolecules, cells, and biomembranes, focusing on electrochemical methods coupled with spectroscopic, spectrometric, and microscopic techniques.

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“…Schuhmann et al also employed SERS detection on nanostructured gold electrodes to detect single mismatches in DNA using both constant potential and scanning potential melt routines. 63 Targets were labeled with the Cy3 dye, and probe densities of 2 ´10 12 −3 10 12 molecules cm −2 were employed. Melts were carried out in 10 mM phosphate buffer containing 450 mM K 2 SO 4 .…”

Section: Electrochemical Melting Of Dna Monolayersmentioning

confidence: 99%

Review—Electrical Manipulation of DNA Self-Assembled Monolayers: Electrochemical Melting of Surface-Bound DNA

West

2020

J. Electrochem. Soc.

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DNA provides a powerful framework for the development of biosensors, DNA chips, bioelectronics, and other established and emerging technologies. Many of these applications involve DNA self-assembled monolayers (SAM) on conducting surfaces where the high molecular density, the two-dimensional nature of the interface, and the limited mobility of the strands significantly impact the behavior of the DNA. The unique steric and electrostatic conditions present in the SAM dominate hybridization, melting, and motion of the tethered oligonucleotides. At neutral pH the charged sugar-phosphate backbone makes the DNA sensitive to the electric fields present in the electrical double-layer. Electrode charge provides a means of modifying the reactivity of DNA monolayers; facilitating enhanced rates of hybridization, controlling orientation, and inducing melting (i.e. denaturation). Understanding the effects of electric fields on DNA monolayers is a prerequisite to the optimization of next generation DNA biosensors and other applications that take advantage of DNA’s selective self-assembly. This mini-review will give an overview of the ways in which electrochemical control can be used to manipulate DNA SAMs. In particular, the process of electric field-assisted melting of DNA, i.e. electrochemical melting, will be reviewed. Electrochemical melting has the potential for providing biophysical insights and for the development of new diagnostic applications.

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Monitoring Potential‐Induced DNA Dehybridization Kinetics for Single Nucleotide Polymorphism Detection by using In Situ Surface Enhanced Raman Scattering

Cited by 7 publications

References 27 publications

DNA Electrostatics: From Theory to Application

DNA Electrostatics: From Theory to Application

In Situ and Operando Techniques for Investigating Electron Transfer in Biological Systems

Review—Electrical Manipulation of DNA Self-Assembled Monolayers: Electrochemical Melting of Surface-Bound DNA

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