Monitoring structural features, biocompatibility and biological efficacy of gamma-irradiated methotrexate-loaded spray-dried microparticles

Oliveira, Anderson Rodrigues de; Mesquita, Philippe C.; Machado, Paula Renata Lima; Farias, Kléber Juvenal Silva; Almeida, Yêda Medeiros Bastos de; Fernandes-Pedrosa, Matheus de Freitas; Cornélio, Alianda Maira; Egito, Eryvaldo Sócrates Tabosa do; Silva-Júnior, Arnóbio Antônio da

doi:10.1016/j.msec.2017.06.013

Cited by 9 publications

(7 citation statements)

References 61 publications

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“…The parabolic model fitted the experimental data much better ( r = 0.95) (Figure 2E), which suggested a drug release controlled by diffusion at the first moment. However, the erosion of polymeric matrix of particles should also be considered for release of residual entrapped drug, which could occur in a second moment [44,48,49,51].…”

Section: Discussionmentioning

confidence: 99%

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Improving Encapsulation of Hydrophilic Chloroquine Diphosphate into Biodegradable Nanoparticles: A Promising Approach against Herpes Virus Simplex-1 Infection

et al. 2018

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Chloroquine diphosphate (CQ) is a hydrophilic drug with low entrapment efficiency in hydrophobic nanoparticles (NP). Herpes simplex virus type 1 (HSV-1) is an enveloped double-stranded DNA virus worldwide known as a common human pathogen. This study aims to develop chloroquine-loaded poly(lactic acid) (PLA) nanoparticles (CQ-NP) to improve the chloroquine anti- HSV-1 efficacy. CQ-NP were successfully prepared using a modified emulsification-solvent evaporation method. Physicochemical properties of the NP were monitored using dynamic light scattering, atomic force microscopy, drug loading efficiency, and drug release studies. Spherical nanoparticles were produced with modal diameter of <300 nm, zeta potential of −20 mv and encapsulation efficiency of 64.1%. In vitro assays of CQ-NP performed in Vero E6 cells, using the MTT-assay, revealed different cytotoxicity levels. Blank nanoparticles (B-NP) were biocompatible. Finally, the antiviral activity tested by the plaque reduction assay revealed greater efficacy for CQ-NP compared to CQ at concentrations equal to or lower than 20 µg mL−1 (p < 0.001). On the other hand, the B-NP had no antiviral activity. The CQ-NP has shown feasible properties and great potential to improve the antiviral activity of drugs.

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Section: Discussionmentioning

confidence: 99%

“…The pairwise comparisons of the analytical data were performed using the Student’s t-test. For the antiviral assay and cell viability, the multiple comparisons were performed using analysis of variance (ANOVA) at p = 0.05 significance level, followed by Bonferroni’s test and Dunnett’s test [43,44].…”

Section: Methodsmentioning

confidence: 99%

Improving Encapsulation of Hydrophilic Chloroquine Diphosphate into Biodegradable Nanoparticles: A Promising Approach against Herpes Virus Simplex-1 Infection

et al. 2018

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“…γ-ray irradiation assures enough high energy for a powerful penetration in the materials [19], and it has been proven to act as an uncomplicated and efficient sterilization method for food package fields [20]. CS is susceptible to degradation when subjected to γ-ray irradiation [21], which could be concerned with the excitation and formation of radical [22].…”

Section: Introductionmentioning

confidence: 99%

60Co γ-ray Irradiation Crosslinking of Chitosan/Graphene Oxide Composite Film: Swelling, Thermal Stability, Mechanical, and Antibacterial Properties

et al. 2018

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Abstract:In this paper, chitosan (CS)/graphene oxide (GO) composite films were prepared, and the effect of γ-ray irradiation on the properties of composite films was investigated. The irradiation crosslinking reaction occurred in composite films with the existence of acetic acid, and the properties changed upon the various irradiation dose. The swelling degree of the composite film with 0 wt % GO decreased with the increasing of the irradiation dose, but the swelling degree of which with GO increased instead. The thermal stability increased with the increasing of the irradiation dose, but the effect of the irradiation on the thermal stability weakened as the increasing of the content of GO, due to the enhanced irradiation resistance performance. The tensile strength increased firstly and decreased subsequently with the increasing of the irradiation dose and the content of GO. Composite films showed the enhanced antibacterial activity against Bacillus subtilis, compared to Escherichia coli and Staphylococcus aureus. The antibacterial activity weakened with the increasing of the content of GO. The antibacterial activity was relatively stronger when the irradiation dose was 20 KGy. In addition, the structural, crystal, and morphological properties of composite films were characterized by FT-IR, XRD, and SEM. It is worth noting that the GO was pre-functionalized via KH560 for the better compatibility with CS matrix.

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“…De Oliveira et al investigated effects of gamma irradiation on spray‐dried Methotrexate (MTX)‐loaded PLGA microparticles. [ 29 ] The sphericity of nonloaded PLGA microparticles decreased and formed some toroidal microparticles, which was attributed to free radical formation after irradiation. However, the smooth surface and size of the microparticles seems remained unchanged.…”

Section: Resultsmentioning

confidence: 99%

Development of PLGA‐PEG‐COOH and Gelatin‐Based Microparticles Dual Delivery System and E‐Beam Sterilization Effects for Controlled Release of BMP‐2 and IGF‐1

Bai

Moeinzadeh

Kim

et al. 2020

Part & Part Syst Charact

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A poly(lactic‐co‐glycolic acid)‐poly(ethylene glycol)‐carboxyl (PLGA‐PEG‐COOH) and gelatin‐based microparticles (MPs) dual delivery system for release of bone morphogenetic protein‐2 (BMP‐2) and insulin‐like growth factor‐1 (IGF‐1) is developed. The delivery system is characterized based on its morphology, loading capacity, encapsulation efficiency, and release kinetics. The effects of electron beam (EB) sterilization on BMP‐2 and IGF‐1‐loaded MPs and their biological effects are examined. The synergistic effect of a controlled dual release of BMP‐2 and IGF‐1 on osteogenesis of human mesenchymal stem cells (MSCs) is evaluated. Encapsulation efficiency of growth factors into gelatin and PLGA‐PEG‐COOH MPs is in the range of 64.78% to 76.11%. E‐beam sterilized growth factor delivery systems are effective in significantly promoting osteogenesis of MSCs, although E‐beam sterilization decreases the bioactivity of growth factors in MPs by ≈22%. BMP‐2 release behavior from gelatin MPs/PEG hydrogel shows a faster release (52.7%) than that of IGF‐1 from the PLGA‐PEG‐COOH MPs/PEG hydrogel (27.3%). The results demonstrate that the gelatin and PLGA‐PEG‐COOH MPs‐based delivery system can realize temporal release of therapeutic biomolecules by incorporating different growth factors into distinct microparticles. EB sterilization is an accessible method for sterilizing growth factors‐loaded carriers, which can pave the way for implementing growth factor delivery in clinical applications.

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Monitoring structural features, biocompatibility and biological efficacy of gamma-irradiated methotrexate-loaded spray-dried microparticles

Cited by 9 publications

References 61 publications

Improving Encapsulation of Hydrophilic Chloroquine Diphosphate into Biodegradable Nanoparticles: A Promising Approach against Herpes Virus Simplex-1 Infection

Improving Encapsulation of Hydrophilic Chloroquine Diphosphate into Biodegradable Nanoparticles: A Promising Approach against Herpes Virus Simplex-1 Infection

60Co γ-ray Irradiation Crosslinking of Chitosan/Graphene Oxide Composite Film: Swelling, Thermal Stability, Mechanical, and Antibacterial Properties

Development of PLGA‐PEG‐COOH and Gelatin‐Based Microparticles Dual Delivery System and E‐Beam Sterilization Effects for Controlled Release of BMP‐2 and IGF‐1

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