Monitoring surfactant mediated defence of gastrointestinal Proteus mirabilis DMTMMK1 against pathogenic consortia of Vibrio cholerae

Kannan, Suganya; Ashokkumar, Krithika; Krishnamoorthy, Govindan; Dhasayan, Asha; Murugan, Marudhamuthu

doi:10.1039/c7ra01934c

Cited by 16 publications

(13 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The biofilm image analysis portrayed the reduction in biofilm formation and disruption of preformed biofilms in the presence of biosurfactant. The biosurfactant treatment in the preformed biofilms of V. harveyi efficiently crumbled the biofilm architecture when compared with the untreated control as portrayed in V. cholerae . The outcome we perceived here is comparable to what has been stated before for V. harveyi and V. parahaemolyticus with the treatment of ethanolic extract of the red seaweed Gracilaria fisheri .…”

Section: Discussionsupporting

confidence: 80%

“…The disintegrated biofilm architecture reveals the effectiveness of biosurfactant, which is due to the disturbance in interbacterial adhesion facilitated by the negative charge of the MK3 biosurfactant. The cell surface morphology confers a selective advantage toward bacterial virulence by aiding the process of adhesion toward the host cell and evasion against immune system . In this study, we demonstrated that the biosurfactant could damage the cell wall and were able to destroy the entire cell structure, this would provide a more effective strategy of the biosurfactant to treat against V. harveyi .…”

Section: Discussionmentioning

confidence: 76%

See 1 more Smart Citation

Effect of biosurfactant derived from Vibrio natriegens MK3 against Vibrio harveyi biofilm and virulence

et al. 2019

Self Cite

View full text Add to dashboard Cite

Vibrio harveyi is a marine luminous pathogen, which causes biofilm-mediated infections, pressures the search for an innovative alternate approach to strive against vibriosis in aquaculture. This study anticipated to explore the effect of glycolipid biosurfactant as an antipathogenic against V. harveyi to control vibriosis. In this study, 27 bacterial strains were isolated from marine soil sediments. Out of these, 11 strains exhibited surfactant activity and the strain MK3 showed high emulsification index. The potent strain was identified as Vibrio natriegens and named as V. natriegens MK3. The extracted biosurfactant was purified using high-performance liquid chromatography and it was efficient to decrease the surface tension of the growth medium up to 21 mN/m. The functional group and composition of the biosurfactant were determined by Fourier-transform infrared spectroscopy and nuclear magnetic resonance spectroscopy spectral studies and the nature of the biosurfactant was identified as glycolipid. The surfactant was capable of reducing the biofilm formation, bioluminescence, extracellular polysaccharide synthesis, and quorum sensing in marine shrimp pathogen V. harveyi. The antagonistic effect of biosurfactant was evaluated against V. harveyi-infected brine shrimp Artemia salina. This study reveals that biosurfactant can be considered for the management of biofilmrelated aquatic infections.

show abstract

Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 76%

Effect of biosurfactant derived from Vibrio natriegens MK3 against Vibrio harveyi biofilm and virulence

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…The surfactant activity was quantitatively evaluated by the emulsification index (EI 24 ) and surface tension determination. For the molecular identification of the best biosurfactant producer strain, the 16S rRNA region was amplified using the universal primers 27F/1492R 30 . PCR products were purified and sequenced.…”

Section: Methodsmentioning

confidence: 99%

“…Culture conditions and biosurfactant preparation were carried out as described by Kannan et al . 30 . Acid precipitated biosurfactant was subjected to repeated extractions with an ethyl acetate–methanol mixture (2:1, v/v).…”

Section: Methodsmentioning

confidence: 99%

Liposome encapsulated surfactant abetted copper nanoparticles alleviates biofilm mediated virulence in pathogenic Pseudomonas aeruginosa and MRSA

Kannan

Solomon

Krishnamoorthy

et al. 2021

Sci Rep

Self Cite

View full text Add to dashboard Cite

In the present study lipopeptide biosurfactant with high emulsification capacity produced by human skin bacterium Paenibacillus thiaminolyticus was purified and subjected to FTIR and NMR spectral analysis which gave evidence of the active characteristics of the surfactant. To augment the antivirulent potential further, the mixer of copper and copper oxide nanoparticles (CuNPs) was synthesized, and characterized by UV–Visible spectroscopy, SEM-EDAX, TEM, and Zeta analysis. Here, we attempted to enhance the antimicrobial and antibiofilm activity with the assistance of encapsulated preparation of lipopeptide and CuNPs in multilamellar liposomes. The proposed mechanism of action of lipopeptide and CuNPs liposomal preparation negatively influences the cell metabolism, secreted virulence such as staphyloxanthin, pyocyanin, and extracellular polysaccharides. The significant decline in the growth of MRSA and P. aeruginosa in both planktonic form and biofilm by lipopeptide and CuNPs treatment were visualized using scanning electron microscopy and High content screening imaging system. In vivo studies revealed that treatment with lipopeptide and CuNPs in multilamellar liposomes extended the lifespan of infected Caenorhabditis elegans by about 75%. Therefore, this study typifies lipopeptide and CuNPs could credibly be a substantial substitute over conventional antibiotics in averting the biofilm associated pathogenesis of MRSA and P. aeruginosa.

show abstract

“…After adding 2 mL of 0.85% saline to the collection tubes, the rectal swab samples were vortexed for 10 min at room temperature and allowed to stand for 5 min, according to a previously reported protocol with minor modifications [17] . Next, 10 μL supernatant was taken and inoculated on MacConkey agar (Difco Laboratories, Detroit, MI, USA) for overnight culturing at 37ºC.…”

Section: Isolation and Identification Of E Coli Strainsmentioning

confidence: 99%

Çin’in Sincan Bölgesindeki Buzağılardan İzole Edilen Escherichia coli’nin Filogenetik Gruplandırması ve Antimikrobiyal Direnç Profili

Zhang¹,

Li²,

Zhang³

et al. 2019

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

The widespread multidrug-resistant Escherichia coli strains have caused a severe challenge to animal health and the development of breeding industries. The purpose of this study was to investigate the phylogenetic grouping and antimicrobial resistance profiles of E. coli isolated from diarrheic calves in Xinjiang province, China. In this study, a total of 379 E. coli strains were isolated from 379 rectal swab samples of diarrheic calves. They were further analyzed their phylogenetic groupings by multiplex PCR, and were clustered into four phylogenetic groups, A (36.1%), B1 (17.4%), B2 (15.6%), and D (30.9%). All E. coli isolates were tested for their susceptibility to 15 antimicrobial agents by Kirby-Bauer (KB) method. The isolates showed the highest resistance rates against ampicillin (64.9%), followed by streptomycin (59.4%), tetracycline (53.8%), sulfamethoxazole/ trimethoprim (50.9%), chloramphenicol (45.6%), kanamycin (44.1%) and enrofloxacin (42.0%). E. coli isolates exhibited lower resistance to ceftazidime (15.0%) and polymyxin (12.6%). The resistance genes blaTEM, blaOXA, mcr-1, strA-strB, aadA, tet(A), tet(B), and tet(C) were detected in 68.3% (168/246), 27.2% (67/246), 14.6% (7/48), 51.1% (115/225), 24.9% (56/225), 51.5% (105/204), 44.6% (91/204), and 7.8% (16/204) of E. coli isolates, respectively. These results demonstrate that prevalent multi-drug resistance and high level of antimicrobial resistance genes exist among E. coli from Xinjiang diarrheic calves and pose a potential public health concern.

show abstract

Monitoring surfactant mediated defence of gastrointestinal Proteus mirabilis DMTMMK1 against pathogenic consortia of Vibrio cholerae

Abstract: Schematic representation of the biosurfactant production and evaluation of anti-pathogenic potential.

Cited by 16 publications

References 33 publications

Effect of biosurfactant derived from Vibrio natriegens MK3 against Vibrio harveyi biofilm and virulence

Effect of biosurfactant derived from Vibrio natriegens MK3 against Vibrio harveyi biofilm and virulence

Liposome encapsulated surfactant abetted copper nanoparticles alleviates biofilm mediated virulence in pathogenic Pseudomonas aeruginosa and MRSA

Çin’in Sincan Bölgesindeki Buzağılardan İzole Edilen Escherichia coli’nin Filogenetik Gruplandırması ve Antimikrobiyal Direnç Profili

Contact Info

Product

Resources

About