Monitoring the Antibacterial Activity of Hibiscus sabdariffa Extracts

Abstract

This paper focuses on studying the antibacterial effect ofHibiscus sabdariffaextracts, a plant known in traditional medicine for its beneficial effects on the body.Hibiscus sabdariffaextracts were done in pure water, 50% ethyl alcohol, and 96% ethyl alcohol; the concentrations used to determine antibacterial activity were 1:1, 1:5, 1:10, 1:25, 1:50, noted cu 1, 2, 3, 4, 5. The antibacterial effect of the extracts was tested using the impregnated disk-diffusion method on bacteriaEscherichia coliATCC 25922,Salmonella typhimuriumATCC 14028,Enterobacter clocaeATCC 13047,Klepsiella pneumoniaeATCC 13833,Staphylococcus aureusATCC 29213,Yersinia enterocoliticaATCC 9610. We ascertained that the most efficient extracts were those obtained using 96% ethyl alcohol, 1:1 concentration onEscherichia coliATCC 25922 andEnterobacter clocaeATCC 13047 strains, where the inhibitory action presented average values between 5.43 mm and 3.43 mm. In the case ofKlepsiella pneumoniaeATCC 13833 andYersinia enterocoliticaATCC 9610 strains, the extracts recorded minimum or zero values. The antibacterial activity ofHibiscus sabdariffadepended on their concentration, on the solvent used, as well as on the resistance of the bacteria under study to the bioactive compounds in them.

Evaluation of Antibacterial Activity of Zobo and Bay Leaf Extracts on Enteropathogenic Bacteria

“…each at 200 mg/mL. Results similar to those obtained from this study were also seen in that of Panaitescu and Lengyel [24] in which H. sabdariffa extracts were found to inhibit growth in E. coli, S. typhi, K. pneumonia and S. aureus used in the study. Inhibitory concentrations were 4, 10, 20 and 100% respectively, while inhibitory zone diameters ranged within 0.1 and 5.0 mm.…”

“…The result also showed that out of the four concentrations of the dry hot aqueous extract, only the 0.25 µg/mL concentration was able to show 14.2±0.0 mm inhibition on S. aureus, while the concentrations of 0.25 µg/mL and 0.125 µg/mL were the only two concentrations of the dry alcohol that showed levels of sensitivity with zone diameters of 29.3±1.0 and 25.2±0.0, respectively. The antimicrobial activities of zobo leaf extracts have been reported by previous studies [22,23,24,25,26,27]. In the study of Salem et al [22], it was shown to inhibit S. aureus, K. pneumoniae and E. coli, at minimum concentrations ranging from 0.30 to1.30±0.2 mg/mL for the three organisms.…”

Hibiscus sabdariffa Extract Inhibits Adhesion, Biofilm Initiation and Formation in Candida albicans

“…Experimental evaluation of their pharmacological use can provide evidence based alternative therapies for herbal drug and/or discovery of novel drug targets. Hibiscus is often taken as a beverage in many countries owing to its medicinal properties [9,10,20]. In this study, we provide evidence for the anti-microbial property of Hs extract with special emphasis on its effect on C. albicans growth and biofilm formation.…”

“…The sensitivity against the tested bacteria differed, with B. atrophaeus being the most sensitive with inhibition zone of approx 1.5 cm in DMSO based extract and 1.2 cm in aqueous extract, while the least sensitive was E. coli with a zone of approximately 1.0 cm in both the extracts. Previous studies have shown the anti-bacterial effect of Hs calyces extract using different solvents such as methanol or ethanol [10,25].…”

“…One such plant known for its various medicinal properties is Hibiscus sabdariffa (Hs). This plant has traditionally been used as a food, herbal drink (both as cold and hot beverages), flavoring agent, fiber source and as a herbal medicine for its anti-bacterial, anti-oxidant, diuretic, anticholesterol, anti-diabetic, anti-hypertensive and anti-cancer activities [9,10]. The methanolic and aqueous extract of Hibiscus calyces have shown anti-bacterial activity against cariogenic Streptomyces mutants [11] and the aqueousmethanolic extract showed favourable effect against various pathogenic bacteria [12].…”