Monitoring the effects of fibrinogen concentration on blood coagulation using quartz crystal microbalance (QCM) and its comparison with thromboelastography

Lakshmanan, Ramji S.; Efremov, Vitaly; Cullen, Sinéad M.; Byrne, Barry; Killard, Anthony J.

doi:10.1117/12.2017394

Cited by 3 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At the same time the value of ∆Γ increases, indicating increased viscosity. Our results are in agreement with and confirm the results of Lakshmanan et al [ 38 , 42 ] and Hussain et al [ 25 ] by showing that signal changes depend on the fibrinogen concentration and that ∆f changes more with raising fibrinogen concentration than ∆Γ ( see grey dotted slope lines Figure 3 a).…”

Section: Resultssupporting

confidence: 93%

Utilisation of Quartz Crystal Microbalance Sensors with Dissipation (QCM-D) for a Clauss Fibrinogen Assay in Comparison with Common Coagulation Reference Methods

Oberfrank

Drechsel

Sinn

et al. 2016

Sensors

View full text Add to dashboard Cite

The determination of fibrinogen levels is one of the most important coagulation measurements in medicine. It plays a crucial part in diagnostic and therapeutic decisions, often associated with time-critical conditions. The commonly used measurement is the Clauss fibrinogen assay (CFA) where plasma is activated by thrombin reagent and which is conducted by mechanical/turbidimetric devices. As quartz crystal microbalance sensors with dissipation (QCM-D) based devices have a small footprint, can be operated easily and allow measurements independently from sample transportation time, laboratory location, availability and opening hours, they offer a great opportunity to complement laboratory CFA measurements. Therefore, the objective of the work was to (1) transfer the CFA to the QCM-D method; (2) develop an easy, time- and cost-effective procedure and (3) compare the results with references. Different sensor coatings (donor’s own plasma; gold surface) and different QCM-D parameters (frequency signal shift; its calculated turning point; dissipation signal shift) were sampled. The results demonstrate the suitability for a QCM-D-based CFA in physiological fibrinogen ranges. Results were obtained in less than 1 min and in very good agreement with a standardized reference (Merlin coagulometer). The results provide a good basis for further investigation and pave the way to a possible application of QCM-D in clinical and non-clinical routine in the medical field.

show abstract

Section: Resultssupporting

confidence: 93%

Utilisation of Quartz Crystal Microbalance Sensors with Dissipation (QCM-D) for a Clauss Fibrinogen Assay in Comparison with Common Coagulation Reference Methods

Oberfrank

Drechsel

Sinn

et al. 2016

Sensors

View full text Add to dashboard Cite

show abstract

“…The viscoelastic changes in blood alter the resonance parameters such as frequency and dissipation (damping factor), and this analysis is called quartz crystal with dissipation (QCM-D). Moreover, since the surface of the electrode can be modified chemically with different types of ligands and proteins, the application of QCM-D to study hemostasis parameters such as platelet aggregation (Sinn et al, 2010), quick time prothrombin time (Cheng et al, 1998;Muller et al, 2009;Hussain, 2016b), prothrombinase induced clotting time (PiCT) (Hussain, 2015), tissue factor effect on the blood coagulation (Lakshmanan et al, 2016), determination of fibrinogen (1-6 g/L) and heparin concentration (Lakshmanan et al, 2013), detection of Factor VIII (Yao et al, 2013), and real time measurements of blood coagulation density and immune complement activation on artificial surfaces have been previously reported (Andersson et al, 2005). During the blood coagulation process, when the fibrins are generated from fibrinogen decomposition due to thrombin, blood viscosity increases and the frequency shifts both because of dissipation and increased mass absorption at the surface of the QCM enabling real time monitoring of blood coagulation.…”

Section: Poc Tests Based On Electromechanical Measurementsmentioning

confidence: 99%

Technology Advancements in Blood Coagulation Measurements for Point-of-Care Diagnostic Testing

Aria

Erten

Yalçın

2019

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

In recent years, blood coagulation monitoring has become crucial to diagnosing causes of hemorrhages, developing anticoagulant drugs, assessing bleeding risk in extensive surgery procedures and dialysis, and investigating the efficacy of hemostatic therapies. In this regard, advanced technologies such as microfluidics, fluorescent microscopy, electrochemical sensing, photoacoustic detection, and micro/nano electromechanical systems (MEMS/NEMS) have been employed to develop highly accurate, robust, and cost-effective point of care (POC) devices. These devices measure electrochemical, optical, and mechanical parameters of clotting blood. Which can be correlated to light transmission/scattering, electrical impedance, and viscoelastic properties. In this regard, this paper discusses the working principles of blood coagulation monitoring, physical and sensing parameters in different technologies. In addition, we discussed the recent progress in developing nanomaterials for blood coagulation detection and treatments which opens up new area of controlling and monitoring of coagulation at the same time in the future. Moreover, commercial products, future trends/challenges in blood coagulation monitoring including novel anticoagulant therapies, multiplexed sensing platforms, and the application of artificial intelligence in diagnosis and monitoring have been included.

show abstract

Measurement of the viscoelastic properties of blood plasma clot formation in response to tissue factor concentration-dependent activation

et al. 2016

Self Cite

View full text Add to dashboard Cite

The coagulation of blood plasma in response to activation with a range of tissue factor (TF) concentrations was studied with a quartz crystal microbalance (QCM), where frequency and half width at half maximum (bandwidth) values measured from the conductance spectrum near resonant frequency were used. Continuous measurement of bandwidth along with the frequency allows for an understanding of the dissipative nature of the forming viscoelastic clot, thus providing information on the complex kinetics of the viscoelastic changes occurring during the clot formation process. Using a mathematical model, these changes in frequency and bandwidth have been used to derive novel QCM parameters of effective elasticity, effective mass density and rigidity factor of the viscoelastic layer. The responses of QCM were compared with those from thromboelastography (TEG) under identical conditions. It was demonstrated that the nature of the clot formed, as determined from the QCM parameters, was highly dependent on the rate of clot formation resulting from the TF concentration used for activation. These parameters could also be related to physical clot characteristics such as fibrin fibre diameter and fibre density, as determined by scanning electron microscopic image analysis. The maximum amplitude (MA) as measured by TEG, which purports to relate to clot strength, was unable to detect these differences.

show abstract

Monitoring the effects of fibrinogen concentration on blood coagulation using quartz crystal microbalance (QCM) and its comparison with thromboelastography

Cited by 3 publications

References 0 publications

Utilisation of Quartz Crystal Microbalance Sensors with Dissipation (QCM-D) for a Clauss Fibrinogen Assay in Comparison with Common Coagulation Reference Methods

Utilisation of Quartz Crystal Microbalance Sensors with Dissipation (QCM-D) for a Clauss Fibrinogen Assay in Comparison with Common Coagulation Reference Methods

Technology Advancements in Blood Coagulation Measurements for Point-of-Care Diagnostic Testing

Measurement of the viscoelastic properties of blood plasma clot formation in response to tissue factor concentration-dependent activation

Contact Info

Product

Resources

About