Monitoring the penetration and accumulation of gold nanoparticles in rat skin <i>ex vivo</i> using surface-enhanced Raman scattering spectroscopy

Xiong, Honglian; Guo, Zhouyi; Zhong, Huiqing; Ji, Yanhong

doi:10.1142/s1793545816500267

Cited by 7 publications

(4 citation statements)

References 48 publications

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“…Penetration and accumulation of gold nanoparticles can be also monitored using a surfaceenhanced Raman scattering (SERS) technique. Therefore, SERS spectroscopy has the potential to monitor the dynamics of nanoparticle penetration and accumulation within the tissues [116].…”

Section: Genotoxicity and Mutagenicitymentioning

confidence: 99%

Biocompatibility and Cytotoxicity of Gold Nanoparticles: Recent Advances in Methodologies and Regulations

Kus‐Liśkiewicz

Fickers

Tahar

2021

IJMS

161

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Recent advances in the synthesis of metal nanoparticles (MeNPs), and more specifically gold nanoparticles (AuNPs), have led to tremendous expansion of their potential applications in different fields, ranging from healthcare research to microelectronics and food packaging. The properties of functionalised MeNPs can be fine-tuned depending on their final application, and subsequently, these properties can strongly modulate their biological effects. In this review, we will firstly focus on the impact of MeNP characteristics (particularly of gold nanoparticles, AuNPs) such as shape, size, and aggregation on their biological activities. Moreover, we will detail different in vitro and in vivo assays to be performed when cytotoxicity and biocompatibility must be assessed. Due to the complex nature of nanomaterials, conflicting studies have led to different views on their safety, and it is clear that the definition of a standard biosafety label for AuNPs is difficult. In fact, AuNPs’ biocompatibility is strongly affected by the nanoparticles’ intrinsic characteristics, biological target, and methodology employed to evaluate their toxicity. In the last part of this review, the current legislation and requirements established by regulatory authorities, defining the main guidelines and standards to characterise new nanomaterials, will also be discussed, as this aspect has not been reviewed recently. It is clear that the lack of well-established safety regulations based on reliable, robust, and universal methodologies has hampered the development of MeNP applications in the healthcare field. Henceforth, the international community must make an effort to adopt specific and standard protocols for characterisation of these products.

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Section: Genotoxicity and Mutagenicitymentioning

confidence: 99%

Biocompatibility and Cytotoxicity of Gold Nanoparticles: Recent Advances in Methodologies and Regulations

Kus‐Liśkiewicz

Fickers

Tahar

2021

IJMS

161

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“…A number of studies have used several analytical and imaging techniques to investigate the dermal penetration potential of NM and have shown a limited ability for nAu and nTiO 2 to penetrate the skin in vivo and in vitro . − In these studies, NM were detected by silver staining, surface enhanced Raman spectroscopy, mass spectroscopy and electron microscopy, and electron microscopy energy dispersive X-ray analysis, with no evidence for transdermal penetration. Sadrieh et al reported the presence of nTiO 2 particles in the dermis following a sub chronic dermal exposure in mini pigs; however, contamination during sample preparation, or cross contamination between samples, could not be ruled out .…”

Section: Discussionmentioning

confidence: 99%

Enhanced Dark-Field Hyperspectral Imaging and Spectral Angle Mapping for Nanomaterial Detection in Consumer Care Products and in Skin Following Dermal Exposure

Boyadzhiev

Trevithick-Sutton

et al. 2020

Chem. Res. Toxicol.

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Consumer personal care products, and cosmetics containing nanomaterials (NM), are increasingly available in the Canadian market. Current Canadian regulations do not require product labeling for ingredients that are present in the nanoscale. As a result, unless voluntarily disclosed, it is unclear which products contain NM. The enhanced dark-field hyperspectral imaging (EDF-HSI) coupled with spectral angle mapping (SAM) is a recent technique that has shown much promise for detection of NM in complex matrices. In the present study, EDF-HSI was used to screen cosmetic inventories for the presence of nano silver (nAg), nano gold (nAu), and nano titanium dioxide (nTiO 2 ). In addition, we also assessed the potential of EDF-HSI as a tool to detect NM in skin layers following application of NM products in vitro on commercially available artificial skin constructs (ASCs) and in vivo on albino hairless SKH-1 mouse skin. Spectroscopic analysis positively detected nAu (4/9 products) and nTiO 2 (7/13 products), but no nAg (0/6 products) in a subset of the cosmetics. The exposure of ASCs for 24 h in a Franz diffusion cell system to a diluted cosmetic containing nTiO 2 revealed penetrance of nTiO 2 through the epidermal layers and was detectable in the receptor fluid. Moreover, both single and multiple applications of nTiO 2 containing cosmetics on the dorsal surface of SKH-1 mice resulted in detectable levels of trace nTiO 2 in the layers of the skin indicating that penetrance of NM was occurring after each application of the product. The current study demonstrates the sensitivity of EDF-HSI with SAM mapping for qualitative detection of NM present in cosmetic products per se and very low levels in complex biological matrices on which these products are applied.

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“…The penetration of SERS-active silver [ 148 , 223 , 257 ] and gold [ 258 , 259 ] nanoparticles has been successfully studied by tracking the appearance of the SERS signal at a certain skin depth. The release of coated silver nanoparticles within the skin has also been demonstrated by the appearance of the SERS spectra [ 148 , 223 ], whose shape and intensity are very different from the spontaneous Raman scattering spectrum of intact skin ( Figure 7 D).…”

Section: Surface-enhanced Raman Scattering (Sers) Microscopymentioning

confidence: 99%

Optical Methods for Non-Invasive Determination of Skin Penetration: Current Trends, Advances, Possibilities, Prospects, and Translation into In Vivo Human Studies

Darvin

2023

Pharmaceutics

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Information on the penetration depth, pathways, metabolization, storage of vehicles, active pharmaceutical ingredients (APIs), and functional cosmetic ingredients (FCIs) of topically applied formulations or contaminants (substances) in skin is of great importance for understanding their interaction with skin targets, treatment efficacy, and risk assessment—a challenging task in dermatology, cosmetology, and pharmacy. Non-invasive methods for the qualitative and quantitative visualization of substances in skin in vivo are favored and limited to optical imaging and spectroscopic methods such as fluorescence/reflectance confocal laser scanning microscopy (CLSM); two-photon tomography (2PT) combined with autofluorescence (2PT-AF), fluorescence lifetime imaging (2PT-FLIM), second-harmonic generation (SHG), coherent anti-Stokes Raman scattering (CARS), and reflectance confocal microscopy (2PT-RCM); three-photon tomography (3PT); confocal Raman micro-spectroscopy (CRM); surface-enhanced Raman scattering (SERS) micro-spectroscopy; stimulated Raman scattering (SRS) microscopy; and optical coherence tomography (OCT). This review summarizes the state of the art in the use of the CLSM, 2PT, 3PT, CRM, SERS, SRS, and OCT optical methods to study skin penetration in vivo non-invasively (302 references). The advantages, limitations, possibilities, and prospects of the reviewed optical methods are comprehensively discussed. The ex vivo studies discussed are potentially translatable into in vivo measurements. The requirements for the optical properties of substances to determine their penetration into skin by certain methods are highlighted.

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Monitoring the penetration and accumulation of gold nanoparticles in rat skin ex vivo using surface-enhanced Raman scattering spectroscopy

Cited by 7 publications

References 48 publications

Biocompatibility and Cytotoxicity of Gold Nanoparticles: Recent Advances in Methodologies and Regulations

Biocompatibility and Cytotoxicity of Gold Nanoparticles: Recent Advances in Methodologies and Regulations

Enhanced Dark-Field Hyperspectral Imaging and Spectral Angle Mapping for Nanomaterial Detection in Consumer Care Products and in Skin Following Dermal Exposure

Optical Methods for Non-Invasive Determination of Skin Penetration: Current Trends, Advances, Possibilities, Prospects, and Translation into In Vivo Human Studies

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