MONITORING THE RESISTANCE PROFILE OF Staphylococcus sp. AND mecA GENE DETECTION OF Staphylococcus aureus FROM LACTATING COWS IN A DAIRY FARMING PROPERTY

Abstract

The main mastitis causing agent is the Staphylococcus, and among them, the most pathogenic species is the Staphylococcus aureus. The present paper aims to verify the frequency of mastistis causing Staphylococcus aureus, trace the susceptibility profile from these to antibiotics and detect the mecA gene in Staphylococcus aureus from a dairy property in Umuarama-PR. Isolated samples used were taken from lactating cows in a seven months period. After the samples were isolated in blood agar and respectively identified, these were kept at-20 ºC to further analysis (antibiograms, polymerase chain reaction to detect the mecA gene, beta-lactam detection and minimum inhibitory concentration detection). 189 samples were collected, and, from these, 30,68% showed no growth, 39,15% were negative coagulase Staphylococcus, 5,29% Staphylococcus aureus, 7,40% Streptococcus and 17,46% other microorganisms (BGP, BGN, Corynebacterium and yeast). The antibiotics that showed higher resistance levels were sulfonamide, penicillin, ampicilin and tetracycline, meanwhile, the ones that showed higher sensibility levels were sulpha+trimethopim, gentamicin, ceftiofure and enrofloxacin. In the samples, 86,41% of the Staphylococcus sp. and 60% of the Staphylococcus aureus were multiresistant. All of the S. aureus samples were negative to the mecA gene and positive to the beta-lactam test.