Monitoring tissue engineered constructs and protocols with laboratory-based x-ray phase contrast tomography

Savvidis, Savvas; Gerli, M; Pellegrini, Marco; Massimi, Lorenzo; Hagen, Charlotte K.; Endrizzi, Marco; Atzeni, Alessia; Ogunbiyi, Olumide; Turmaine, Mark; Smith, Elizabeth S.; Fagiani, Claudio; Selmin, Giulia; Urbani, Luca; Durkin, Natalie; Shibuya, Soichi; Coppi, Paolo De; Olivo, Alessandro

doi:10.1016/j.actbio.2022.01.022

Cited by 15 publications

(13 citation statements)

References 37 publications

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“…Using the same parameters and procedures, we then scanned a more complex sample: a decellularised piglet esophagus (a so-called “scaffold”), which had been prepared in the context of tissue engineering research (Savvidis et al 32 ), to demonstrate the ability to extract three-modal images from a single flyscan (Fig. 4 ).…”

Section: Resultsmentioning

confidence: 99%

Cycloidal-spiral sampling for three-modal x-ray CT flyscans with two-dimensional phase sensitivity

Lioliou

Morgó

Marathe

et al. 2022

Sci Rep

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We present a flyscan compatible acquisition scheme for three-modal X-Ray Computed Tomography (CT) with two-dimensional phase sensitivity. Our approach is demonstrated using a “beam tracking” setup, through which a sample’s attenuation, phase (refraction) and scattering properties can be measured from a single frame, providing three complementary contrast channels. Up to now, such setups required the sample to be stepped at each rotation angle to sample signals at an adequate rate, to prevent resolution losses, anisotropic resolution, and under-sampling artefacts. However, the need for stepping necessitated a step-and-shoot implementation, which is affected by motors’ overheads and increases the total scan time. By contrast, our proposed scheme, by which continuous horizontal and vertical translations of the sample are integrated with its rotation (leading to a “cycloidal-spiral” trajectory), is fully compatible with continuous scanning (flyscans). This leads to greatly reduced scan times while largely preserving image quality and isotropic resolution.

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Section: Resultsmentioning

confidence: 99%

Cycloidal-spiral sampling for three-modal x-ray CT flyscans with two-dimensional phase sensitivity

Lioliou

Morgó

Marathe

et al. 2022

Sci Rep

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“…This sample was chosen as it contains challenging biological tissue, with small features which conventional X-ray imaging techniques struggle to resolve. More details about the design of the data acquisition experiment can be found in 9 . The region of interest covered 413 × 143 pixels, with a 50 µm pixel size, for each projection angle, with 1200 such angles from 0 • to 359.7 • .…”

Section: Resultsmentioning

confidence: 99%

Comparison of different Gaussian fitting and phase contrast retrieval methods

Djurabekova

Buchanan

Doherty

et al. 2022

Preprint

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Edge illumination is an X-ray phase contrast imaging technique that allows for the recovery of differential phase and dark-field (also known as scattering) contrast, in addition to conventional attenuation based contrast. These signals are retrieved by analysing the illumination profile of a series of beamlets across a detector. This can provide a wealth of information about the sample, especially biological tissues which produce little contrast using conventional attenuation radiography. To perform signal retrieval, Gaussian curves can be fit to the illumination profiles using a variety of methods. In this work, we compare the most prominent of these methods in terms of their contrast to noise ratio as well as computational times to investigate their effect on the retrieved projection data as well as the final reconstructions.

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“…A series of samples were then scanned for tomography: a multi-material phantom, consisting of HDPE, PP and cornflower cylinders; a short-fibre reinforced composite; a pouch cell battery and a tissue-engineered oesophagus (See Savvidis et al for oesophageal sample preparation 14 ). The regular acquisition parameters were set as 8 dithering steps, 7 illumination curve points, 720 angular projections and 1.2 seconds of exposure, however, the high attenuation of the pouch cell battery meant that 9 illumination curve points were used to increase the statistics of the acquisition, and for the oesophagus where 1000 angular views were used for preserving the spatial resolution.…”

Section: Methodsmentioning

confidence: 99%

X-ray dark-field tomography using edge-illumination

Doherty

Savidis

Astolfo

et al. 2022

Developments in X-Ray Tomography XIV

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X-ray dark-field imaging is used to visualise the ultra-small angle x-ray scattering signal that originates from sub-resolution density fluctuations within the sample microstructure. Dark-field tomography using the edgeillumination x-ray imaging system is presented as a tool for measuring this scattering signal in a sample in three dimensions. Its applicability to different fields is shown through example images of a multi-material phantom, a tissue-engineered oesophagus, a pouch cell battery and a short-fibre reinforced composite material. The multichannel contrast available in edge-illumination helps with material identification, with high contrast at boundaries enhancing dark-field reconstructions.

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Monitoring tissue engineered constructs and protocols with laboratory-based x-ray phase contrast tomography

Cited by 15 publications

References 37 publications

Cycloidal-spiral sampling for three-modal x-ray CT flyscans with two-dimensional phase sensitivity

Cycloidal-spiral sampling for three-modal x-ray CT flyscans with two-dimensional phase sensitivity

Comparison of different Gaussian fitting and phase contrast retrieval methods

X-ray dark-field tomography using edge-illumination

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