Monitoring wound healing of elastic cartilage using multiphoton microscopy

Zhu, Xiaodong; Tang, Yiyan; Chen, J.; Xiong, Shuyuan; Zhuo, Shuangmu

doi:10.1016/j.joca.2013.08.016

Cited by 24 publications

(17 citation statements)

References 27 publications

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“…Figure (B) shows similar morphology of matrix to Figure (A). The characteristic of collagen, which is able to emit comparable SHG and TPEF signals in the rabbit skin or the submucosa, may be one of the reasons to explain the phenomenon that these tissues are feasible to be healed after injuries (Da Costa et al ., ; Luo et al ., ; Chen et al ., ; Jiang et al ., ; Zhu et al ., ). It is noted that almost no elastic fibers which exclusively emit TPEF signal without SHG signal in Figure (B), are observed.…”

Section: Resultsmentioning

confidence: 97%

Multiphoton microscopic imaging of rabbit dorsal skin

Zhu

Hong

et al. 2014

Scanning

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Rabbits are often preferred to be experimental animals during the skin research. The visualizing and understanding the full-thickness structure of rabbit skin has significance in biology, medicine, and animal husbandry. In this study, multiphoton microscopy (MPM) was employed to examine the rabbit skin on the back, which was based on second harmonic generation and two-photon excited fluorescence. High-resolution images were achieved from the fresh, unfixed, and unstained tissues, showing detailed microstructure of the skin without the administration of exogenous contrast agents. The morphology and distribution of the main components of epidermis and dermis, such as keratin, collagen fibers, elastic fibers, and hair follicles, can be distinctly identified in MPM images. Since the changes in these components are tightly related to skin diseases and wound healing, the noninvasive nature of MPM enables it become a valuable tool in skin research for detecting and monitoring.

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Section: Resultsmentioning

confidence: 97%

Multiphoton microscopic imaging of rabbit dorsal skin

Zhu

Hong

et al. 2014

Scanning

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“…Current high-resolution observation at the cellular and molecular level following ischemic stroke is typically accomplished using fluorescent-based microscopy techniques [21,38]. However, these techniques require exogenous fluorescence markers to produce the necessary labeling and contrast.…”

Section: Discussionmentioning

confidence: 99%

“…Such methods have exhibited the ability to detect biological tissues and changes in these tissues with high resolution and high sensitivity. According to previous research, abundant intrinsic fluorophores are present in biological tissues emitting high TPEF signals, such as nicotinamide adenine dinucleotide (NADH), nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), retinol, and tryptophan and its indoleamine derivatives [19][20][21]. These distinctions are not clearly revealed by traditional histopathology.…”

Section: Introductionmentioning

confidence: 99%

Spatial and temporal identification of cerebral infarctions based on multiphoton microscopic imaging

Wang

Lin

et al. 2018

Biomed. Opt. Express

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Ischemic stroke is a leading cause of death and permanent disability worldwide. Middle cerebral artery occlusion (MCAO) of variable duration times could be anticipated to result in varying degrees of injury that evolve spatially over time. Therefore, investigations following strokes require information concerning the spatiotemporal dimensions of the ischemic core as well as of perilesional areas. In the present study, multiphoton microscopy (MPM) based on two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) was applied to image such pathophysiological events. The ischemic time-points for evaluation were set at 6, 24, 48, and 72 hours after MCAO. Our results demonstrated that MPM has the ability to not only identify the normal and ischemic brain regions, but also reveal morphological changes of the cortex and striatum at various times following permanent MCAO. These findings corresponded well with the hematoxylin and eosin (H&E) stained tissue images. With the technologic progression of miniaturized imaging devices, MPM can be developed into an effective diagnostic and monitoring tool for ischemic stroke.

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“…The NLO imaging system used in this study contained a highthroughput scanning inverted Axiovert 200 microscope (LSM 510 META; Zeiss, Jena, Thuringia, Germany). In addition, it contained a mode-locked femtosecond Ti: sapphire laser (110 fs, 76 MHz) operating at 810 nm (Mira 900-F, Coherent, Santa Clara, CA, USA; Zhu et al, 2013). The polarization of laser light is linear.…”

Section: Imaging Instrumentationmentioning

confidence: 99%

Label‐free identification of the microstructure of rat spinal cords based on nonlinear optical microscopy

Liao

Wang

Zhou

et al. 2017

Journal of Microscopy

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The spinal cord is a vital link between the brain and the body and mainly comprises neurons, glial cells and nerve fibres. In this work, nonlinear optical (NLO) microscopy based on intrinsic tissue properties was employed to label-freely analyze the cells and matrix in spinal cords at a molecular level. The high-resolution and high-contrast NLO images of unstained spinal cords demonstrate that NLO microscopy has the ability to show the microstructure of white and grey matter including ventral horn, intermediate area, dorsal horns, ventral column, lateral column and dorsal column. Neurons with various sizes were identified in grey matter by dark spots of nonfluorescent nuclei encircled by cytoplasm-emitting two-photon excited fluorescence signals. Nerve fibres and neuroglias were observed in white matter. Besides, the spinal arteries were clearly presented by NLO microscopy. Using spectral and morphological information, this technique was proved to be an effective tool for label-freely imaging spinal cord tissues, based on endogenous signals in biological tissue. With future development, we foresee promising applications of the NLO technique for in vivo, real-time assessment of spinal cord diseases or injures.

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Monitoring wound healing of elastic cartilage using multiphoton microscopy

Abstract: Our results suggested that MPM has the ability to monitor the wound healing progression of elastic cartilage, based on the visualization of cell growth and proliferation and quantitative characterization of collagen morphology during wound healing.

Cited by 24 publications

References 27 publications

Multiphoton microscopic imaging of rabbit dorsal skin

Multiphoton microscopic imaging of rabbit dorsal skin

Spatial and temporal identification of cerebral infarctions based on multiphoton microscopic imaging

Label‐free identification of the microstructure of rat spinal cords based on nonlinear optical microscopy

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