Mono-methylated histones control PARP-1 in chromatin and transcription

Abstract

PARP-1 is central to transcriptional regulation under both normal and heat stress conditions, with the governing mechanisms yet to be fully understood. Biochemical and ChIP-seq-based analyses showed that PARP-1 binds specifically to active histone marks, particularly H4K20me1. PR-Set7, the sole H4K20 mono-methylase, and PARP-1 mutants undergo developmental arrest during larval-pupal shift in Drosophila . RNA-seq analysis showed a positive correlation between PR-Set7-and PARP-1-dependent gene expression in third-instar larvae, suggesting co-regulation during developmental phases. During heat stress, PARP-1 repositions from the Hsp70 promoter to its gene body, facilitating gene activation. PARP-1 and PR-Set7 are essential for activating Hsp70 and a subset of heat shock genes during heat stress, with a notable increase of H4K20me1 at their gene body. We propose that H4K20me1 plays a critical role in facilitating PARP-1 binding and the regulation of specific genes during both development and heat stress.

Understanding Environmental Consequences of Quarry Operations: Residents’ Perception Study in the Neighbourhood of a Quarry in Osun state, Nigeria

“…Furthermore, the deplorable condition of roads connecting quarry sites to major trunks has been noted, primarily attributed to the transportation of heavy-duty vehicles carrying products from quarries. 20 This creates a ripple effect on infrastructure, impacting both local and regional connectivity. In the context of sustainable development, particularly Sustainable Development Goal 11 (SDG 11), which aims to make cities and human settlements inclusive, safe, resilient, and sustainable, there is a pressing need to address the challenges posed by quarry activities.…”