Monoallelic expresion of the odourant receptor gene and axonal projection of olfactory sensory neurones

Ishii, Takeshi; Serizawa, Shou; Kohda, Atsushi; Nakatani, Hiroko; Shiroishi, Toshihiko; Okumura, Katsuhiko; Iwakura, Yoichiro; Nagawa, Fumikiyo; Tsuboi, Akito; Sakano, Hitoshi

doi:10.1046/j.1365-2443.2001.00398.x

Cited by 91 publications

(60 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This finding is in accordance with previous data showing that the sensory neurons expressing a particular odorant receptor terminate in specific glomeruli. This feature has been shown to be the case both in mammals (Ishii et al, 2001;Ressler et al, 1994;Vassar et al, 1994) and fish (Hansen et al, 2003;Sato et al, 2005;Sato et al, 2007). A functional projection has also been found using voltage-sensitive dyes Friedrich and Korsching, 1997;Friedrich and Korsching, 1998).…”

Section: Selectivitymentioning

confidence: 66%

Visualizing a set of olfactory sensory neurons responding to a bile salt

Døving

Hansson

Bäckström

et al. 2011

Journal of Experimental Biology

View full text Add to dashboard Cite

SUMMARYIn the present study, we exposed the olfactory epithelia of crucian carp, Carassius carassius, and brown trout, Salmo trutta, to dextran coupled with Alexa dyes together with odorants. Dye uptake was severely reduced after pre-exposure to nocodazole, an inhibitor of microtubule polymerization that impairs endocytosis, supporting the hypothesis that odour-activated olfactory receptor molecules undergo endocytosis. Application of the bile acid taurolithocholate, a potent and specific odorant for fish, resulted in the labelling of a sparse (less than 3%) cell population with the typical morphology of ciliated sensory neurons (CSNs) -long dendrites and cell somata deep in the sensory epithelium. The dye was distributed throughout the sensory neuron, also revealing axons and target glomeruli. Stained axons redistribute at the entrance of the olfactory bulb and terminate in two small target areas, a dorsal and a medial one. These results are consistent with the notion that taurolithocholate is detected specifically by a few ciliated sensory neurons. Application of the olfactory epithelium of brown trout to bile acid stained cells with the appearance of CSNs. Application of an alarm agonist, hypxanthine-3-N-oxide, to crucian carp olfactory organ caused staining of another set of sensory neurons. Furthermore, our results show that odour-induced uptake of a dye can serve to identify the subtype of olfactory sensory neurons responding to a particular odorant, and to pinpoint the target regions of these neurons in the olfactory bulb as a first step to elucidating the neuronal network responding to a particular odour.

show abstract

Section: Selectivitymentioning

confidence: 66%

Visualizing a set of olfactory sensory neurons responding to a bile salt

Døving

Hansson

Bäckström

et al. 2011

Journal of Experimental Biology

View full text Add to dashboard Cite

show abstract

“…The MOR28 glomeruli as visualized in the MOR28-IRES-gap-GFP mouse strain constitute a popular model for studies of OSN axon wiring (18)(19)(20)(21)(22)(23)(24). In a first set of experiments, we imaged six bulbs of three littermates at postnatal day (PD) 21 that are homozygous for the gene-targeted mutation MOR28-IRES-gap-GFP (18,19). In the gap-GFP marker, the 20 N-terminal amino acid residues of GAP43 are fused to the N terminus of GFP to target GFP to the plasma membrane (25).…”

Section: Resultsmentioning

confidence: 99%

“…We have assessed quantitatively the positional variability of a total of six types of OR-specific glomeruli: MOR23 (Olfr16), mOR37A (Olfr155), M72 (Olfr160), P2 (Olfr17), MOR256-17 (Olfr15), and MOR28 (Olfr1507). The objective of multiplexing was achieved by repeatedly crossing gene-targeted mouse strains carrying a single tagged OR gene (4,7,(15)(16)(17)(18)(19) until mice were obtained with mutations at four (4OR) or five (5OR) loci. These OR genes reside on different chromosomes, facilitating the generation of quadruple and quintuple mutant mice without the need for meiotic crossovers.…”

Section: Significancementioning

confidence: 99%

Multiplex assessment of the positions of odorant receptor-specific glomeruli in the mouse olfactory bulb by serial two-photon tomography

Zapiec

Mombaerts

2015

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

In the mouse, axons of olfactory sensory neurons (OSNs) that express the same odorant receptor (OR) gene coalesce into one or a few glomeruli in the olfactory bulb. The positions of OR-specific glomeruli are traditionally described as stereotyped. Here, we have assessed quantitatively the positions of OR-specific glomeruli using serial two-photon tomography, an automated method for wholeorgan fluorescence imaging that integrates two-photon microscopy with serial microtome sectioning. Our strategy is multiplexed. By repeated crossing, we generated two strains of mice with genetargeted mutations at four or five OR loci for a total of six ORs: MOR23 (Olfr16), mOR37A (Olfr155), M72 (Olfr160), P2 (Olfr17), MOR256-17 (Olfr15), and MOR28 (Olfr1507). Glomerular imaging relied on intrinsic fluorescence of GFP or DsRed, or on wholemount immunofluorescence with antibodies against GFP, DsRed, or β-gal using the method of immunolabeling-enabled threedimensional imaging of solvent-cleared organs (iDISCO). The highresolution 3D-reconstructed datasets were segmented to identify the labeled glomeruli and to assess glomerular positional variability between the bulbs of one mouse (intraindividual) and among the bulbs of different mice (interindividual). In 26 mice aged 21 or 50 d or 10 wk, we made measurements of the positions of 352 glomeruli. We find that positional variability of glomeruli correlates with the OR: For instance, the medial MOR28 glomerular domain occupies a surface area that is an order of magnitude larger than the surface area of the medial MOR23 glomerular domain. Our results quantify the level of precision that is delivered by the mechanisms of OSN axon wiring, differentially for the various OSN populations expressing distinct OR genes.olfaction | glomerulus | gene targeting | axon guidance | olfactory sensory neuron

show abstract

“…Each olfactory sensory neuron (OSN) expresses only one functional OR gene in a monoallelic manner (Chess et al, 1994;Malnic et al, 1999;Ishii et al, 2001;Serizawa et al, 2003), and expression of each OR gene is confined to a restricted area in the olfactory epithelium (OE) (Ressler et al, 1993;Vassar et al, 1993;Strotmann et al, 1994;Sullivan et al, 1996). Furthermore, OSNs expressing a given OR gene project their axons to a specific set of glomeruli in the olfactory bulb (OB) (Ressler et al, 1994;Vassar et al, 1994;Mombaerts et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Continuous and Overlapping Expression Domains of Odorant Receptor Genes in the Olfactory Epithelium Determine the Dorsal/Ventral Positioning of Glomeruli in the Olfactory Bulb

Miyamichi¹,

Serizawa²,

Kimura³

et al. 2005

J. Neurosci.

284

327

View full text Add to dashboard Cite

In mammals, olfactory signals received by odorant receptors (ORs) in the olfactory epithelium (OE) are converted to a topographical map of activated glomeruli in the olfactory bulb (OB). It has been reported that the OE can be divided into four topographically distinct zones and that olfactory sensory neurons (OSNs) expressing a particular OR gene are randomly distributed within one zone. Here, we analyzed 80 different class II OR genes for their expression patterns in the OE by in situ hybridization. It was found that the expression area in the OE does not always fit into one of the four conventional zones. Expression areas are specific to each OR gene and are arranged in an overlapping and continuous manner in the OE. We also analyzed a spatial relationship between the OE and the OB for OSN projection. Our transgenic as well as DiI retrograde staining experiments demonstrated that the dorsal/ventral arrangement of glomeruli in the OB is correlated with the expression areas of corresponding ORs along the dorsomedial/ventrolateral axis in the OE. The present study indicates that the OR gene choice may be more restricted by the OSN location in the OE than what has been thought.

show abstract

Monoallelic expresion of the odourant receptor gene and axonal projection of olfactory sensory neurones

Cited by 91 publications

References 18 publications

Visualizing a set of olfactory sensory neurons responding to a bile salt

Visualizing a set of olfactory sensory neurons responding to a bile salt

Multiplex assessment of the positions of odorant receptor-specific glomeruli in the mouse olfactory bulb by serial two-photon tomography

Continuous and Overlapping Expression Domains of Odorant Receptor Genes in the Olfactory Epithelium Determine the Dorsal/Ventral Positioning of Glomeruli in the Olfactory Bulb

Contact Info

Product

Resources

About