Monoamine-dependent Production of Reactive Oxygen Species Catalyzed by Pseudoperoxidase Activity of Human Hemoglobin

Kawano, Tomonori; Pinontoan, Reinhard; Hosoya, Hishoshi; Muto, Sshoshi

doi:10.1271/bbb.66.1224

Cited by 39 publications

(30 citation statements)

References 51 publications

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“…2B). In other models of heme proteins, such as HRP 17,20,21 and human hemoglobin, 22 similar observations were reported. Compound III was further converted to irreversibly inactivated form of heme proteins known as P-670, if incubated over 1 h with excess dose of H 2 O 2 (molar ratio 100:1; Fig.…”

Section: Spectroscopic Analysessupporting

confidence: 80%

Hydrogen peroxide-independent generation of superoxide catalyzed by soybean peroxidase in response to ferrous ion

Kimura

Kawano

2015

Plant Signaling & Behavior

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Section: Spectroscopic Analysessupporting

confidence: 80%

Hydrogen peroxide-independent generation of superoxide catalyzed by soybean peroxidase in response to ferrous ion

Kimura

Kawano

2015

Plant Signaling & Behavior

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“…•-) [52][53]. Although superoxide ion itself is not considered cytotoxic, it spawns toxic derivatives such as hydroxyl radicals and hypohalous acid [54].…”

Section: Discussionmentioning

confidence: 99%

Assessment of Biochemical and Histopathological Effects of Crude Venom of Cone Snail <i>Conus flavidus</i> on <i>albino</i> Mice

Abou-Elezz¹

2017

IJEE

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Abstract:The genus Conus is equipped with a unique venomous mixture of conopeptides which secreted for predation and defense purposes. This work is aiming to explore and determine the effect of the crude venom of Conus flavidus, a wormhunting cone snail inhabiting the Red Sea, on the oxidant/ antioxidant system in mice using some oxidative stress biomarker assays. In addition to assess its histopathological effects on some treated organs. The LC 50 were detected for the crude venom using the hemolytic assay (16.7 mg/ml) and male albino mice were injected intraperitoneally with ½ LC 50 (8.3 mg/kg B.Wt). Biochemically, after 2, 4, 6, 12, 24 hours of injection the results revealed significant inhibition of superoxide dismutase (SOD) and catalase (CAT) activities in blood and liver in almost all time intervals comparing with control one. However, it showed elevation in lipid peroxide content (LPC), protein carbonyl content (PCC), nitric oxide level (NO) reduced glutathione content (GSH) and total antioxidant capacity (TAC) contents of both blood and liver in almost all time intervals. Histopathologicaly, liver and heart were dissected after 1, 3 and 7 days of injection. The treated liver showed vacuolar degeneration, karyolosis and pyknosis, mild blood sinusoidal congestion and centrilobular necrosis. The treated heart illustrated degenerated myofibrils, pyknosis, edema, blood vessel congestion, loss of striation normal construction and fascicular pattern in the myocardium. These results revealed that C. flavidus crude venom has distinct effects upon the oxidant/antioxidant cellular system and degenerative pathological effects in some tissues of treated animals, proving that this venom may contain bioactive peptides, which could be purified and used for further pharmacological and drug discovery investigations in the future.

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“…This finding suggests the presence of potential endogenous antioxidant compounds, with a reduction of the total oxidant status. Reductase systems may catalyze the reduction of ferric iron Fe 3+ to the ferrous Fe 2+ state (22), suggesting a role for antioxidant response in the pathogenesis of EAOC. Antioxidant activity may be upregulated to address the redox imbalance in a subset of endometriotic cells, which may be exposed to sublethal levels of ROS and able to adapt to cell survival (27)(28)(29).…”

Section: Discussionmentioning

confidence: 99%

Cyst fluid hemoglobin species in endometriosis and its malignant transformation: The role of metallobiology

Iwabuchi¹,

Yoshimoto

Shinmoto

et al. 2016

Oncology Letters

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Abstract. The aim of the present study was to assess the relative concentrations of hemoglobin (Hb) species in endometriosis as a possible indicator of malignancy. Electronic absorption spectroscopy was employed to quantify the Hb species present in the cyst fluid collected from 8 patients with endometriosis-associated ovarian cancer (EAOC), and compared with those present in the cyst fluid of 35 patients with benign endometriotic cysts. The 620/580 nm ratio in the electronic absorption spectrum, which was used as a surrogate indicator of the methemoglobin (metHb)/(oxyhemoglobin+metHb) ratio, was measured in each cyst fluid by ultraviolet/visible grating spectrophotometric microplate reader. The optimal cutoff value was defined according to the analysis of receiver operating characteristic (ROC) curve. The sensitivity and specificity of detection were calculated on the basis of the cutoff value to differentiate EAOC from endometriosis. The 620/580 nm ratio of cyst fluid in EAOC patients was much lower than that measured in women with benign cysts (0.389±0.266 vs. 0.666±0.188, P=0.021). ROC curve analysis performed using 0.35 as the optimal cutoff value indicated that the 620/580 nm ratio had a sensitivity, specificity, positive predictive value (PPV) and negative predictive value of 62.5, 100.0, 100.0 and 92.1%, respectively, in the diagnosis of EAOC. In conclusion, metHb is one of the most abundant Hb species in benign cysts, and the absorption 620/580 nm ratio of cyst fluid exhibits high specificity and PPV as a surveillance test for the early detection of malignant transformation of endometriosis. Thus, metallobiology highlights diverse features involved in Hb homeostasis and the pathogenesis of malignant transformation of endometriosis.

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Monoamine-dependent Production of Reactive Oxygen Species Catalyzed by Pseudoperoxidase Activity of Human Hemoglobin

Cited by 39 publications

References 51 publications

Hydrogen peroxide-independent generation of superoxide catalyzed by soybean peroxidase in response to ferrous ion

Hydrogen peroxide-independent generation of superoxide catalyzed by soybean peroxidase in response to ferrous ion

Assessment of Biochemical and Histopathological Effects of Crude Venom of Cone Snail <i>Conus flavidus</i> on <i>albino</i> Mice

Cyst fluid hemoglobin species in endometriosis and its malignant transformation: The role of metallobiology

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Monoamine-dependent Production of Reactive Oxygen Species Catalyzed by Pseudoperoxidase Activity of Human Hemoglobin

Cited by 39 publications

References 51 publications

Hydrogen peroxide-independent generation of superoxide catalyzed by soybean peroxidase in response to ferrous ion

Hydrogen peroxide-independent generation of superoxide catalyzed by soybean peroxidase in response to ferrous ion

Assessment of Biochemical and Histopathological Effects of Crude Venom of Cone Snail &lt;i&gt;Conus flavidus&lt;/i&gt; on &lt;i&gt;albino&lt;/i&gt; Mice

Cyst fluid hemoglobin species in endometriosis and its malignant transformation: The role of metallobiology

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Assessment of Biochemical and Histopathological Effects of Crude Venom of Cone Snail <i>Conus flavidus</i> on <i>albino</i> Mice