Monoamine oxidase B layer-by-layer film fabrication and characterization toward dopamine detection

Miyazaki, Celina M.; Pereira, Tamyris Paschoal; Mascagni, Daniela Branco Tavares; Moraes, Marli L.

doi:10.1016/j.msec.2015.08.042

Cited by 24 publications

(11 citation statements)

References 54 publications

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“…Anionic and zwitterionic phospholipids [1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3phosphocholine (POPC); N-glutaryl-phosphatidylethanolamine (NGPE); 1,2dihexadecanoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (sodium salt) (DPPG); 1hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1′-rac-glycerol) (sodium salt) (POPG); 1,2-dimyristoyl-sn-glycero-3-phospho-(1-rac-glycerol) (DMPG); lyso-1heptadecanoyl-sn-glycero-3-phosphocholine (LPC)] and Langmuir-Blodgett films composed by arachidic acid have been the most tested for the development of enzymatic biosensors (Table 1); the only enzymes used on those studies were tyrosinase (Tyr), MP, monoamine oxidase B (MAO-B), HRP, acetylcholinesterase (AChE) and glucose oxidase (GOD). Still, the number of studies (Table 1) is very limited [ [24], [25], [26], [27], [28], [29], [30]]. Cationic and other zwitterionic phospholipids, such as 1,2-di-(9Zoctadecenoyl)-sn-glycero-3-phosphoethanolamine (DOPE), 1,2-di-(9Z-octadecenoyl)-3trimethylammonium-propane (DOTAP) and 1,2-distearoyl-sn-glycero-3phosphoethanolamine (DSPE) have not yet been tested for preservation, stabilization and immobilization of enzymes.…”

Section: Introductionmentioning

confidence: 99%

Biosensor for direct bioelectrocatalysis detection of nitric oxide using nitric oxide reductase incorporated in carboxylated single-walled carbon nanotubes/lipidic 3 bilayer nanocomposite

Gomes

Maia

Loureiro

et al. 2019

Bioelectrochemistry

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An enzymatic biosensor based on nitric oxide reductase (NOR; purified from Marinobacter hydrocarbonoclasticus) was developed for nitric oxide (NO) detection. The biosensor was prepared by deposition onto a pyrolytic graphite electrode (PGE) of a nanocomposite constituted by carboxylated single-walled carbon nanotubes (SWCNTs), a lipidic bilayer [1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine (DOPE), 1,2-di-(9Z-octadecenoyl)-3-trimethylammonium-propane (DOTAP), 1,2-distearoyl-snglycero-3-phosphoethanolamine-polyethylene glycol (DSPE-PEG)] and NOR. NOR direct electron transfer and NO bioelectrocatalysis were characterized by several electrochemical techniques. The biosensor development was also followed by scanning electron microscopy and Fourier transform infrared spectroscopy. Improved enzyme stability and electron transfer (1.96 × 10 −4 cm.s −1 apparent rate constant) was obtained with the optimum SWCNTs/(DOPE:DOTAP:DSPE-PEG)/NOR) ratio of 4/2.5/4 (v/v/v), which biomimicked the NOR environment. The PGE/[SWCNTs/(DOPE:DOTAP:DSPE-PEG)/NOR] biosensor exhibited a low Michaelis-Menten constant (4.3 μM), wide linear range (0.44-9.09 μM), low detection limit (0.13 μM), high repeatability (4.1% RSD), reproducibility (7.0% RSD), and stability (ca. 5 weeks). Selectivity tests towards Larginine, ascorbic acid, sodium nitrate, sodium nitrite and glucose showed that these compounds did not significantly interfere in NO biosensing (91.0 ± 9.3%-98.4 ± 5.3% recoveries). The proposed biosensor, by incorporating the benefits of biomimetic features of the phospholipid bilayer with SWCNT's inherent properties and NOR bioelectrocatalytic activity and selectivity, is a promising tool for NO.

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Section: Introductionmentioning

confidence: 99%

Biosensor for direct bioelectrocatalysis detection of nitric oxide using nitric oxide reductase incorporated in carboxylated single-walled carbon nanotubes/lipidic 3 bilayer nanocomposite

Gomes

Maia

Loureiro

et al. 2019

Bioelectrochemistry

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“…However, DA coexists in the extracellular fluids of the central nervous system with two electrochemical interferents, ascorbic acid (AA) and uric acid (UA), both present in higher concentration and exhibiting oxidation potential very close to DA [9]. In this way, the development of modified electrodes with high selectivity to DA has been focus of extensively work, including applications of a plenty of materials, as metal nanoparticles [9], conducting polymers [10], carbon nanotubes [11,12], graphene [4,9,10,[13][14][15], specific enzymes [16], among others. For instance, Uzun et al developed a sensor composed by N,N'-bis(indole-3-carboxaldimine)-1,2-diaminocyclohexane onto glassy carbon electrode, which is capable to separate the oxidation peaks of DA, AA and UA by the differential pulse voltammetry with limit of detection of DA equal to 0.18 mol L −1 [17].…”

Section: Introductionmentioning

confidence: 99%

Layer-by-layer composite film of nickel phthalocyanine and montmorillonite clay for synergistic effect on electrochemical detection of dopamine

Lucena

Miyazaki

Shimizu

et al. 2018

Applied Surface Science

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a b s t r a c t Dopamine (DA) abnormal levels are related to diseases which makes important the development of fast, reliable, low-cost and sensitive devices for diagnosis and pharmaceutical controls. Nanostructured film composite of sodium montmorillonite clay (Na + MMT) and nickel phthalocyanine (NiTsPc) was selfassembled by layer-by-layer (LbL) technique and applied as electrochemical sensor for DA in the presence of common natural interferents as ascorbic acid (AA) and uric acid (UA). Three different LbL architecture films were investigated: LbL films of clay (PEI/Na + MMT) and phthalocyanine (PEI/NiTsPc) in a bilayer structure with a conventional polyelectrolyte (PEI) and a composite film formed by both materials to verify the synergistic effect in the LbL film in a quadri-layer assembly (PEI/Na + MMT/PEI/NiTsPc). Structural characterization indicated molecular level interactions between the layers forming the LbL films. The ITO/(PEI/Na + MMT/PEI/NiTsPc) 10 electrode exhibited a LOD of 1.0 mol L −1 and linear range 5-150 mol L −1 .

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“…The procedure is repeated for the assembly of a multilayered film with different supramolecular architectures on the substrate 18 . The immobilization of different materials such as NPMs, 22,23 biomolecules, 24,25 graphene‐based materials metal 19,20 among others, results in multilayered hybrid materials that present synergistic effect and achieve an optimum condition 26,27 …”

Section: Introductionmentioning

confidence: 99%

An investigation of the synergistic effect between magnetite nanoparticles and polypyrrole in nanostructured layer‐by‐layer films

Lima

Maciel

Ferreira

et al. 2020

J of Applied Polymer Sci

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In this paper, we report the controlled fabrication of layer-by-layer (LbL) films deposited on gold substrates with three different supramolecular architectures using polypyrrole (Ppy) and magnetite nanoparticles (Fe 3 O 4-np), besides conventional poly(allylamine hydrochloride) (PAH) e poly(vinyl sulfonic acid) (PVS) polyelectrolytes, demonstrating the synergistic effect between Ppy and Fe 3 O 4-np such as a result of their interaction. Modified gold electrodes were analyzed by contact angle (wettability), surface plasmon resonance (SPR), raman spectroscopy, cyclic voltammetry, and electrochemical impedance spectroscopy. The (Fe 3 O 4-np/Ppy) 3 architecture was also evaluated by scanning electron microscopy. The modified gold electrodes present more homogeneous covering, higher electron transfer and a decrease of resistance with the incorporation of the nanostructured materials such as Ppy and Fe 3 O 4-np forming (Fe 3 O 4-np/Ppy) 3 LbL film. The results carried out in this study suggest that the (Fe 3 O 4-np/Ppy) 3 LbL film can be applied as a possible electrochemical or optical non-enzymatic sensor for analytical detection.

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Monoamine oxidase B layer-by-layer film fabrication and characterization toward dopamine detection

Cited by 24 publications

References 54 publications

Biosensor for direct bioelectrocatalysis detection of nitric oxide using nitric oxide reductase incorporated in carboxylated single-walled carbon nanotubes/lipidic 3 bilayer nanocomposite

Biosensor for direct bioelectrocatalysis detection of nitric oxide using nitric oxide reductase incorporated in carboxylated single-walled carbon nanotubes/lipidic 3 bilayer nanocomposite

Layer-by-layer composite film of nickel phthalocyanine and montmorillonite clay for synergistic effect on electrochemical detection of dopamine

An investigation of the synergistic effect between magnetite nanoparticles and polypyrrole in nanostructured layer‐by‐layer films

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