1980
DOI: 10.1016/0141-0229(80)90066-6
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Monoamine oxidase electrode in freshness testing of meat

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Cited by 59 publications
(21 citation statements)
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“…Standards were obtained from Sigma Chemical Company (St. Louis, MO, USA). Ki values were calculated according to Karube et al [1980] by substituting the contents (µmol/g wet muscle) of principle adenine nucleotides and their related compounds in the following equation:…”
Section: Nucleotide Analysismentioning
confidence: 99%
“…Standards were obtained from Sigma Chemical Company (St. Louis, MO, USA). Ki values were calculated according to Karube et al [1980] by substituting the contents (µmol/g wet muscle) of principle adenine nucleotides and their related compounds in the following equation:…”
Section: Nucleotide Analysismentioning
confidence: 99%
“…Objective estimation of freshness is required in fish markets and food industries for manufacture of high quality products. Indicators of fish freshness, such as volatile acids, 2 l amines, 3 ) ammonia, 4 > catalase activity 5 l and pH 6 > have been proposed. Saito et a!., 7 > introduced the "K value" as an indicator of fish freshness: the K value is defined as the rate ofthe sum ofHxR and Hx to that of ATP and its breakdown products.…”
Section: K1(%)=100(hxr+hx)/(imp+hxr+hx)mentioning
confidence: 99%
“…La DAO comercial exhibe baja actividad enzimática (0.18 U/mg de sólido). El método de inmovilización por entrecruzamiento con glutaraldehído permite obtener alta carga de enzima sobre la membrana y por tanto mejorar la operación del sensor (Carelli et al, 2007); sin embargo, Karube et al, (1980) mencionan que la DAO puede ser desnaturalizada con facilidad en presencia de glutaraldehído. En este contexto, varias metodologías de inmovilización se ensayaron antes de elegir como óptimo el método descrito en el apartado 4.3.6.1.…”
Section: Sensor Enzimático Con La Enzima Diamina Oxidasaunclassified
“…Sin embargo, la TY fue detectada a concentraciones mayores de 0.02 mg/mL, no siendo así para la SM y SD. La actividad enzimática de la DAO es muy dependiente del pH como así lo han mencionado varios autores (Karube et al, 1980, Keow et al, 2007, siendo el pH óptimo diferente para las distintas AB. En este sentido, se evaluó el efecto del pH (entre 6.2 y 9.2) en la actividad catalítica de la enzima inmovilizada sobre HI y PU.…”
Section: Mezclar De 5 µL De Dao a (60unclassified
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