Monocarboxylate Transporter‐1 Mediates the Protective Effects of Neutral‐pH Bicarbonate/Lactate‐Buffered Peritoneal Dialysis Fluid on Cell Viability and Apoptosis

Kuma, Akihiro; Tamura, Masahito; Ishimatsu, Nana; Harada, Yukie; Izumi, Hiroto; Miyamoto, Tetsu; Furuno, Yumi; Nakano, Yoko; Serino, Ryota

doi:10.1111/1744-9987.12476

Cited by 5 publications

(2 citation statements)

References 26 publications

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“…One report used the same two PDFs we used and found that bicarbonate/lactate PDF was less toxic to cell viability and apoptosis of PMCs than lactate PDF [12]. In addition, a clinical study [13] reported that Fibrin degradation products and vascular endothelial growth factor concentrations in effluent were lower when using bicarbonate/lactate PDF than when using lactate PDF.…”

Section: Discussionmentioning

confidence: 98%

Effect of lactate as a peritoneal dialysis fluid buffer on rat peritoneal mesothelial cells

2020

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Background Neutral, low-glucose degradation product (GDP) peritoneal dialysis fluid (PDF) is less damaging to the peritoneum than conventional PDF but is still insufficient for biocompatibility. One remaining issue is the problem of buffering. Methods Using cultured rat peritoneal mesothelial cells (PMCs), the present study examined the difference between the effects of neutral low-GDP lactate PDF and neutral low-GDP bicarbonate/lactate PDF on cells. The effects of lactate stimulation on these cells were also examined. Results Lactate PDF enhanced mRNA expressions of α-smooth muscle actin (αSMA) and type 1 and type 3 collagens and lowered expression of e-cadherin mRNA in PMCs compared to bicarbonate/lactate PDF. Lactate stimulation increased mRNA expressions of αSMA, matrix metalloproteinase 2 (MMP2), and basic fibroblast growth factor (bFGF) and suppressed e-cadherin mRNA expression. Transforming growth factor (TGF)-β1 and TGF-β2 and collagen type 1 and 3 mRNA expressions were also enhanced by lactate stimulation. Conclusions These results suggest that lactate as a PDF buffer may act on PMCs to promote epithelial-mesenchymal transition (EMT) and production of TGF-β, bFGF, and collagen.

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Section: Discussionmentioning

confidence: 98%

Effect of lactate as a peritoneal dialysis fluid buffer on rat peritoneal mesothelial cells

2020

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“…Low-glucose degradation product bicarbonate/lactatebuffered solutions are reported to have less cell toxicity and superior cell viability in vitro, and less neoangiogenesis and impaired ultrafiltration in rat models of PD, as compared with low pH lactate-buffered solutions [8][9][10]. Kuma et al reported a lactate-induced reduction in cell viability, showing that apoptosis of human peritoneal mesothelial cells was markedly induced by L-PDFs, but not by B/L-PDFs, which was attenuated by knockdown of monocarboxylate transporter-1, a cell membrane lactate transporter [11].…”

Section: Introductionmentioning

confidence: 99%

Effects of bicarbonate/lactate-buffered neutral peritoneal dialysis fluids on angiogenesis-related proteins in patients undergoing peritoneal dialysis

et al. 2021

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Background In order to facilitate the safe and long-term delivery of peritoneal dialysis (PD), it is necessary to improve the biocompatibility of peritoneal dialysis fluids (PDFs). The novel bicarbonate/lactate-buffered neutral PDFs (B/L-PDFs) are expected to be improved biocompatible. This study evaluated the biocompatibility of B/L-PDFs by analysis on the profile of angiogenesis-related proteins in drained dialysate of patients undergoing PD. Methods Concentrations of 20 angiogenesis-related proteins in the dialysate were semi-quantitatively determined using a RayBio® Human Angiogenesis Antibody Array and were compared between B/L-PDFs and conventional lactate-buffered neutral PDFs (L-PDFs). Results The expression of growth-related oncogene (GRO α/β/γ), which belongs to the CXC chemokine family, decreased significantly after use of the B/L-PDFs compared to the L-PDFs (P = 0.03). The number of the proteins with lower level in the B/L-PDFs compared with L-PDFs was significantly negatively correlated with the PD duration (Spearman ρ = − 0.81, P = 0.004). Conclusion This study suggested that B/L-PDFs are more biocompatible than conventional PDFs.

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In vitro efficacy and safety of a system for sorbent-assisted peritoneal dialysis

Gelder

Ligabue

Giovanella

et al. 2020

American Journal of Physiology-Renal Physiology

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A system for sorbent-assisted peritoneal dialysis (SAPD) was designed to continuously recirculate dialysate via a tidal mode using a single lumen peritoneal catheter with regeneration of spent dialysate by means of sorbent technology. We hypothesize that SAPD treatment will maintain a high plasma-to-dialysate concentration gradient and increase the mass transfer area coefficient of solutes. Thereby, the SAPD system may enhance clearance while reducing the number of exchanges. Application is envisaged at night as a bedside device (12 kg, nighttime system). A wearable system (2.0 kg, daytime system) may further enhance clearance during the day. Urea, creatinine, and phosphate removal were studied with the daytime and nighttime system ( n = 3 per system) by recirculating 2 liters of spent peritoneal dialysate via a tidal mode (mean flow rate: 50 and 100 mL/min, respectively) for 8 h in vitro. Time-averaged plasma clearance over 24 h was modeled assuming one 2 liter exchange/day, an increase in mass transfer area coefficient, and 0.9 liters ultrafiltration/day. Urea, creatinine, and phosphate removal was 33.2 ± 4.1, 5.3 ± 0.5, and 6.2 ± 1.8 mmol, respectively, with the daytime system and 204 ± 28, 10.3 ± 2.4, and 11.4 ± 2.1 mmol, respectively, with the nighttime system. Time-averaged plasma clearances of urea, creatinine and phosphate were 9.6 ± 1.1, 9.6 ± 1.7, and 7.0 ± 0.9 mL/min, respectively, with the nighttime system and 10.8 ± 1.1, 13.4 ± 1.8, and 9.7 ± 1.6 mL/min, respectively, with the daytime and nighttime system. SAPD treatment may improve removal of uremic toxins compared with conventional peritoneal dialysis, provided that peritoneal mass transport will increase.

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Monocarboxylate Transporter‐1 Mediates the Protective Effects of Neutral‐pH Bicarbonate/Lactate‐Buffered Peritoneal Dialysis Fluid on Cell Viability and Apoptosis

Cited by 5 publications

References 26 publications

Effect of lactate as a peritoneal dialysis fluid buffer on rat peritoneal mesothelial cells

Effect of lactate as a peritoneal dialysis fluid buffer on rat peritoneal mesothelial cells

Effects of bicarbonate/lactate-buffered neutral peritoneal dialysis fluids on angiogenesis-related proteins in patients undergoing peritoneal dialysis

In vitro efficacy and safety of a system for sorbent-assisted peritoneal dialysis

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