Monochromatic light elicited biomass accumulation, antioxidant activity, and secondary metabolite production in callus culture of Operculina turpethum (L.)

Biswal, Bhagyashree; Jena, Biswajit; Giri, Alok Kumar; Acharya, Laxmikanta

doi:10.1007/s11240-022-02274-9

Cited by 14 publications

(6 citation statements)

References 51 publications

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“…According to our results, BL light led to the up-regulation of flavonoid biosynthesis in other plant species, for example, Ajuga bracteosa adventitious roots [50], Curculigo orchioides synseed-derived seedlings [51] and Scrophularia kakudensis shoots [37]. Exposure to BL light of Operculina turpethum [52] and Rhodiola imbricata [53] callus culture also positively affected specialized metabolite production. It resulted in a higher content of flavonoids and total phenolics than other LED light illuminations.…”

Section: Discussionsupporting

confidence: 57%

Light-Emitting Diodes and Liquid System Affect the Caffeoylquinic Acid Derivative and Flavonoid Production and Shoot Growth of Rhaponticum carthamoides (Willd.) Iljin

Skała,

Olszewska,

Tabaka

et al. 2024

Molecules

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Plant in vitro cultures can be an effective tool in obtaining desired specialized metabolites. The purpose of this study was to evaluate the effect of light-emitting diodes (LEDs) on phenolic compounds in Rhaponticum carthamoides shoots cultured in vitro. R. carthamoides is an endemic and medicinal plant at risk of extinction due to the massive harvesting of its roots and rhizomes from the natural environment. The shoots were cultured on an agar-solidified and liquid-agitated Murashige and Skoog’s medium supplemented with 0.1 mg/L of indole-3-acetic acid (IAA) and 0.5 mg/L of 6-benzyladenine (BA). The effect of the medium and different treatments of LED lights (blue (BL), red (RL), white (WL), and a combination of red and blue (R:BL; 7:3)) on R. carthamoides shoot growth and its biosynthetic potential was observed. Medium type and the duration of LED light exposure did not affect the proliferation rate of shoots, but they altered the shoot morphology and specialized metabolite accumulation. The liquid medium and BL light were the most beneficial for the caffeoylquinic acid derivatives (CQAs) production, shoot growth, and biomass increment. The liquid medium and BL light enhanced the content of the sum of all identified CQAs (6 mg/g DW) about three-fold compared to WL light and control, fluorescent lamps. HPLC-UV analysis confirmed that chlorogenic acid (5-CQA) was the primary compound in shoot extracts regardless of the type of culture and the light conditions (1.19–3.25 mg/g DW), with the highest level under R:BL light. BL and RL lights were equally effective. The abundant component was also 3,5-di-O-caffeoylquinic acid, accompanied by 4,5-di-O-caffeoylquinic acid, a tentatively identified dicaffeoylquinic acid derivative, and a tricaffeoylquinic acid derivative 2, the contents of which depended on the LED light conditions.

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Section: Discussionsupporting

confidence: 57%

Light-Emitting Diodes and Liquid System Affect the Caffeoylquinic Acid Derivative and Flavonoid Production and Shoot Growth of Rhaponticum carthamoides (Willd.) Iljin

Skała,

Olszewska,

Tabaka

et al. 2024

Molecules

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“…The white light spectrum was also found to promote isofraxidin content in the roots of Sarcandra glabra seedlings [ 65 ]. Isofraxidin belongs to coumarin, which was also shown to be upregulated in low-red lights [ 66 , 67 ]. Isofraxidin in plant root is synthesized as a bioactive response to abiotic stress.…”

Section: Discussionmentioning

confidence: 99%

Bioactive extracts and association with C and N in Eleutherococcus senticosus subjected to chitosan nanoparticles in contrasting light spectra

et al. 2022

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Bioactive compounds are major reasons for the value of Eleutherococcus senticosus, which can be modified by different lighting spectra. Light-emitting diode (LED) provides lights with specific spectra which can interact with other treatments to impact plant bioactive production. Chitosan nanoparticle (CN) is a biopolymer derived from marine creatures. It’s usage may be a practical approach to cope with uncertainties in secondary metabolites induced by illumination. Carbon (C) and nitrogen (N) cyclings link plant eco-physiological performance and bioactive substance; hence their associations may reveal the mechanism of joint light-CN interaction. In this study, E. senticosus seedlings were raised under artificial lighting spectra from high-pressure sodium (HPS) lamps (44% red, 55% green, 1% blue) and white (44% red, 47% green, 8% blue) and red colored (73% red, 13% green, 14% blue) LED panels. Half of the seedlings received CN and the other half received distilled water as the control. Compared to the HPS spectrum, the red-light induced stronger shoot growth with greater biomass accumulation and higher water uptake but resulted in lower N concentration and biomass ratio in the root. The white light caused more biomass allocated to the root and strengthened stem C concentration. Stem eleutheroside B increased with shoot growth, while root eleutheroside B had a positive association with leaf C and stem protocatechuic acid had a negative association with leaf N. Having the CN treatment in white and red LED lights is recommended for increasing accumulation of bioactive compounds in the shoots and roots of E. senticosus seedlings, respectively.

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“…Perhaps this effect is a specific reaction of tea cells grown at high light intensity, which is characterized by the greatest greening of callus surface. The relationship between chlorophyll accumulation and plant growth has been noted by a number of authors [58,59]. It should also be noted that in the calli of all variants the water content was the same during the passage.…”

Section: Growth Of Tea Callus Culturementioning

confidence: 67%

The Influence of Light Intensity and Duration on the Formation of Primary and Secondary Metabolites in Tea Plant Cell Culture

Zubova,

Goncharuk,

Nechaeva

et al. 2023

Preprint

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Tea plants (Camellia sinensis L.) are economically important horticultural crops for industrial use. The drink (tea) obtained from them is used all over the world, and its functional activity is determined by the polyphenols content and composition. Its accumulation is regulated by the action of many environmental factors. This paper presents data on the different intensity (50, 75, and 100 µmol·m−2·s−1) and duration of light exposure on tea plant in vitro cultures. Changes in morphophysiological characteristics, accumulation of carbohydrates and polyphenols, including their monomeric (phenylpropanoids, flavanols) and oligomeric (proanthocyanidins) forms, were studied. The malondialdehyde and photosynthetic pigments (chlorophyll a and b) contents were also analyzed. In the calli grown in light conditions, chloroplasts and anthocyanoplasts formation was noted, which was especially pronounced under the highest illumination intensity. A significant similarity between the growth of callus cultures, the carbohydrates and polyphenols accumulation at 50 and 75 µmol·m−2·s−1 was established. Which wasn’t characteristic of the calli grown at 100 µmol·m−2·s−1. The stress reaction absence in tea callus cultures has been demonstrated, which may be a consequence of the phenolic antioxidants’ accumulation in them. The obtained data evidence that their potential ability to accumulate primary (carbohydrates) and secondary (polyphenols) metabolites depends on the light exposure intensity and duration.

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Monochromatic light elicited biomass accumulation, antioxidant activity, and secondary metabolite production in callus culture of Operculina turpethum (L.)

Cited by 14 publications

References 51 publications

Light-Emitting Diodes and Liquid System Affect the Caffeoylquinic Acid Derivative and Flavonoid Production and Shoot Growth of Rhaponticum carthamoides (Willd.) Iljin

Light-Emitting Diodes and Liquid System Affect the Caffeoylquinic Acid Derivative and Flavonoid Production and Shoot Growth of Rhaponticum carthamoides (Willd.) Iljin

Bioactive extracts and association with C and N in Eleutherococcus senticosus subjected to chitosan nanoparticles in contrasting light spectra

The Influence of Light Intensity and Duration on the Formation of Primary and Secondary Metabolites in Tea Plant Cell Culture

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