Monoclonal antibodies against bovine immunoglobulins and their use in isotype-specific ELISAs for rotavirus antibody

Zaane, D. van; Ijzerman, Johan

doi:10.1016/0022-1759(84)90011-5

Cited by 67 publications

(30 citation statements)

References 14 publications

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“…We used an antibody capture assay (ACA) for detection of both IgM and IgA, instead of an indirect test as described by Brocchi et al [6]. The ACA approach has among others been used for bovine rotavirus [14], bovine respiratory syncytial virus [15], pseudorabies virus [16] and influenza virus [17] and shown to overcome antibody competition between IgM or IgA, and IgG antibodies [14]. The design of the IgG, IgG "…”

Section: Discussionmentioning

confidence: 99%

Isotype specific ELISAs to detect antibodies against swine vesicular disease virus and their use in epidemiology

Dekker¹,

Hemert-Kluitenberg²,

Baars³

et al. 2002

Epidemiol. Infect.

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Isotype specific ELISAs to detect antibodies against swine vesicular disease, which may help to estimate the moment of infection, were developed and validated on sera from pigs experimentally infected with four different isolates of swine vesicular disease virus. Virus specific IgM antibodies could be detected from days 3–49 and occasionally up to day 91 after infection. IgG1 antibodies were first detected at day 8 and IgG2 at day 11. IgA antibodies coincided with IgG1 antibodies, but antibody titres varied widely. From the results obtained with the sera from the experimentally infected pigs, we calculated the day at which 50% of the pigs had become positive (D50). A D50 of 5, 4, 12, 12 and 24 days was calculated, respectively, for the appearance of antibodies in the virus neutralization test, the IgM, total IgG, IgG1 and IgG2 ELISA. A D50 of 49 days was calculated for the disappearance of IgM antibodies. The isotype specific ELISAs proved to be valuable tools to study the epidemiology of the disease.

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Section: Discussionmentioning

confidence: 99%

Isotype specific ELISAs to detect antibodies against swine vesicular disease virus and their use in epidemiology

Dekker¹,

Hemert-Kluitenberg²,

Baars³

et al. 2002

Epidemiol. Infect.

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“…After incubation of the samples and appropriate washing steps, the bound swine antibodies were detected using either goat anti-swine IgM or goat anti-swine IgG directly conjugated to horseradish peroxidase (product number 04-14-03 or 04-14-02, respectively; KPL, Gaithersburg, MD) or a mouse monoclonal anti-swine IgA (clone K61 1B4, product number MCA638; Serotec, Raleigh, NC) that was subsequently detected using goat anti-mouse IgG directly conjugated with horseradish peroxidase (product number 04-18-02 from KPL). This ELISA format has been previously described as an isotype-specific indirect double-antibody sandwich ELISA (IDAS-ELISA) (35). In the interest of consistency with the published data, we have retained this nomenclature.…”

Section: Methodsmentioning

confidence: 99%

“…The second assay used the same mouse MAb described above but in an assay configuration previously described by van Zaane and colleagues as the isotypespecific antibody capture assay-ELISA (ACA-ELISA) (35). In this configuration, Immulon 2 microtiter plates were coated with sheep anti-mouse IgG (product number AAC10P from Serotec) diluted 1:200 in coating buffer (carbonatebicarbonate, pH 9.6) at 50 l/well (see Fig.…”

Section: Vol 17 2010 Antibody Response Of Swine To Foot-and-mouth Dmentioning

confidence: 99%

IgA Antibody Response of Swine to Foot-and-Mouth Disease Virus Infection and Vaccination

Pacheco

Butler

Jew

et al. 2010

Clin Vaccine Immunol

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Foot-and-mouth disease virus (FMDV) continues to be a significant economic problem worldwide. Control of the disease involves the use of killed-virus vaccines, a control measure developed decades ago. After natural infection, the primary site of replication of FMDV is the pharyngeal area, suggesting that a mucosal immune response is the most effective. Humoral immunity to killed-virus vaccination induces antibodies that can prevent the clinical disease but not local infection. Determining whether infection or vaccination stimulates IgA-mediated local immunity depends on the method of analysis. Different assays have been described to analyze the quality of antibody responses of cattle and swine to FMDV, including indirect double-antibody sandwich enzyme-linked immunosorbent assay (IDAS-ELISA) and antibody capture assay-ELISA (ACA-ELISA). We tested these assays on swine and show that vaccinated animals had FMDV-specific IgM and IgG but no IgA in either serum or saliva. After the infection, both assays detected FMDV-specific IgM, IgG, and IgA in serum. Notably, serum IgA was more readily detected using the ACA-ELISA, whereas IgA was not detected in saliva with this assay. FMDV-specific IgA antibodies were detected in saliva samples using the IDAS-ELISA. These data show that parenterally administered, killed-virus vaccine does not induce a mucosal antibody response to FMDV and illuminates limitations and appropriate applications of the two ELISAs used to measure FMDV-specific responses. Further, the presence of the IgA antivirus in serum correlates with the presence of such antibodies in saliva.

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“…MAb HB65 was purified from hybridoma cell culture medium by affinity chromatography on protein G sepharose and was used for coating of microtiter plates and for conjugation with horseradish peroxidase (HRPO) (27). In previous studies, an antibody capture assay (ACA) format of the ELISA performed better for the detection of virus-specific IgM and IgA, and an indirect double antibody sandwich assay (IDAS) format performed better for the detection of virusspecific IgGi (4,23). Therefore, we developed an ACA-ELISA for the detection of influenza NP specific IgM and IgA, and an IDAS-ELISA for the detection of influenza NP-specific IgGi.…”

mentioning

confidence: 99%

Systemic and Mucosal Isotype-Specific Antibody Responses in Pigs to Experimental Influenza Virus Infection

Heinen¹,

Nieuwstadt²,

Pol³

et al. 2000

Viral Immunology

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The immunoglobulin isotype-specific responses in serum and at the respiratory mucosa of pigs after a primary infection with influenza virus were studied. To do this, we developed an aerosol challenge model for influenza in specified pathogen-free (SPF) pigs and isotype-specific enzyme-linked immunosorbent assays (ELISAs). Ten-week-old pigs were inoculated without anesthesia in the nostrils with an aerosol of the field isolate influenza A/swine/Neth/St. Oedenrode/96 (H3N2). The infection caused acute respiratory disease that closely resembled the disease observed in some outbreaks of influenza among finishing pigs, which were not complicated by bacterial infections. Pigs showed clinical signs characterized by fever, dyspnea, and anorexia. At necropsy on postinfection days 1 and 2, an exudative endobronchitis was observed throughout the lung. Viral antigen was present in the epithelial cells of the bronchi and bronchioli and virus was isolated from bronchioalveolar and nasal lavage fluids and from pharyngeal swabs until 5 days after infection. With the isotype-specific ELISAs, viral nucleoprotein specific immunoglobulin (Ig) M, IgG1, and IgA antibody responses were measured in serum and bronchioalveolar and nasal lavage fluids. To determine whether the antibodies were produced and secreted at the respiratory mucosa or were serum-derived, the specific activity (ie, the ratio of antibody titer to Ig concentration) was calculated for each isotype. The IgA and interestingly also a substantial part of the IgG1 antibody response in pigs upon infection with influenza virus was shown to be a mucosal response. Local production of specific IgA in the nasal mucosa, and of specific IgA and IgG1 in the lung was demonstrated. These results indicate that protective efficacy of vaccination can be improved by an immunization procedure that preferentially stimulates a mucosal immune response. The aerosol challenge model in SPF pigs and the isotype-specific ELISAs that we developed can be useful for evaluating various strategies to improve efficacy of porcine influenza vaccines and to study the immune mechanisms underlying the observed protection.

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Monoclonal antibodies against bovine immunoglobulins and their use in isotype-specific ELISAs for rotavirus antibody

Cited by 67 publications

References 14 publications

Isotype specific ELISAs to detect antibodies against swine vesicular disease virus and their use in epidemiology

Isotype specific ELISAs to detect antibodies against swine vesicular disease virus and their use in epidemiology

IgA Antibody Response of Swine to Foot-and-Mouth Disease Virus Infection and Vaccination

Systemic and Mucosal Isotype-Specific Antibody Responses in Pigs to Experimental Influenza Virus Infection

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