Monoclonal antibodies against murine leukemia viruses: Identification of six antigenic determinants on the p15(E) and gp70 envelope proteins

Lostrom, Mark E.; Stone, Mary R.; Tam, Milton R.; Burnette, W N; Pinter, Abraham; Nowinski, Robert C.

doi:10.1016/0042-6822(79)90557-9

Cited by 122 publications

(54 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Three different antibodies to P,5(E) are designated as aP15(E)a, aPi5(E)b, and aPis(E)c; and tvo different antibodies to GP70 are designated aGP7ob anid aGP70e. The isolation, characterization, and specificity of these antibodies have been previously described (26 Protein determinations. Protein concentrations of ultrafiltrates were determined by the method of Lowry et al (28).…”

mentioning

confidence: 99%

Inhibitors of monocyte responses to chemotaxins are present in human cancerous effusions and react with monoclonal antibodies to the P15(E) structural protein of retroviruses.

Cianciolo¹,

Hunter²,

Silva³

et al. 1981

J. Clin. Invest.

116

View full text Add to dashboard Cite

mentioning

confidence: 99%

Inhibitors of monocyte responses to chemotaxins are present in human cancerous effusions and react with monoclonal antibodies to the P15(E) structural protein of retroviruses.

Cianciolo¹,

Hunter²,

Silva³

et al. 1981

J. Clin. Invest.

116

View full text Add to dashboard Cite

“…Negative XC test on X-MuLV-inoculated mouse cells also rules out the phenotypic mixing. Cross-reactions between p15 (E)'s of E-MuLV and X-MuLV have been noted (Lostrom et al, 1979). Further analyses with monoclonal specific antisera would be necessary to resolve the question.…”

Section: Discussionmentioning

confidence: 99%

Ecotropic and Xenotropic Type C Retroviruses Associated with Reticulum Cell Neoplasms of SJL/J Mice

Chang

Horgan

Bandyopadhyay

et al. 1981

Journal of General Virology

View full text Add to dashboard Cite

SUMMARYAn ecotropic type C retrovirus (D 1-MuLV) isolated from SJL/J mice was injected into neonatally thymectomized SJL/J mice. There was no acceleration of development of reticulum cell neoplasm (RCN) in these mice as compared with the control, uninoculated but similarly thymectomized group. The incidence of RCN at 10 and 11 months after injection was 14.6% and 12-5% respectively. Two female mice inoculated with D1-MuLV developed mammary adenocareinoma. There was persistence of high titres of the ecotropic virus associated with RCN and mammary tumour of SJL/J mice. Xenotropic virus (X-MuLV) was detected in spleens of normal SJL/J mice at ages 6 and 12 months (60% and 80% respectively) but not at other ages. The X-MuLV isolated from SJL/J mouse embryo cell cultures treated with 5-iodo-2'-deoxyuridine (SJL-MEF-X-MuLV) and that isolated from a spontaneous RCN (SJL-RCN-X-MuLV) were compared with NZB-X-MuLV (NZB mouse origin) and AT124-X-MuLV (NIH Swiss mouse origin) in regard to their host range, ion and primer-template preference by reverse transcriptase, virus interference and neutralization characteristics. Cross-neutralization and gp70 competitive radioimmunoassays showed that D 1-MuLV is more closely related to AKR-MuLV than to Rauscher-MuLV and appears to share some virus envelope antigens with SJL-X-MuLVs. The type-specific gag gene product (p12) from Balb :virus-2 and NZB-X-MuLV was used in competitive radioimmunoassay, and SJL-RCN-XMuLV was found to be more closely related to Balb :virus-2 (MuLV-X ~) than to NZB-X-MuLV (MuLV-X~). INTRODUCTIONThe SJL/J strain of mouse is known to develop Hodgkin's-like type B reticulum cell neoplasm (RCN) at advanced age. The characteristics of an N-tropic type C retrovirus isolated from normal, as well as from RCN-bearing, lymphoid tissues of SJL/J mice were described previously (Chang et al., 1974). When this virus (designated D1-MuLV) was

show abstract

“…Screening was performed by ELISA with purified OmpF trimers and monomers at 1 p.g per well. Ascites was generated by the protocol of Lostrom et al (23).…”

Section: Methodsmentioning

confidence: 99%

Acquisition of apparently intact and unmodified lipopolysaccharides from Escherichia coli by Bdellovibrio bacteriovorus

et al. 1992

View full text Add to dashboard Cite

The ability of Bdellovibrio bacteriovorus to relocalize the OmpF major outer membrane porins from its Escherichia coli prey to its own outer membranes is diminished in prey expressing smooth lipopolysaccharide (S-LPS). Since porins exist in the membrane complexed with LPS, we examined the LPS associated with relocalized porin to determine whether it had been acquired intact, mixed or replaced with Edegovibrio LPS, or derivatized by the bdellovibrios. The relocalized trimers were found associated with the same LPS onrginally bound to them in the E. coli. The bulk-phase LPS from bdellovibrios grown on various chemotypes of rough prey was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to determine whether more than the trimer-bound LPS was acquired by the bdellovibrios. This analysis revealed bands ofBdeilovibrio LPS matching the LPS chemotype of the prey. One or two other bands were identical in migration to the LPS of prey-independent mutants of B. bacterovorus and represented bdellovibrio-synthesized LPS. The LPS of bdellovibrios grown on prey with radiolabeled lipid A showed radioactivity only in gel band positions identical with those of the prey's LPS. The amount-of this prey-derived LPS was shown by enzyme-linked immunosorbent assay to reach a constant value during the purification of the bdellovibrios, and it represented approximately 25% of the total Bdellovibrio LPS. Immunoelectron microscopy confirmed the presence of prey-derived LPS on the cell surface of bdellovibrios, and no evidence could be found for bdellovibrio-induced modifications of the relocalized prey LPS.

show abstract

Monoclonal antibodies against murine leukemia viruses: Identification of six antigenic determinants on the p15(E) and gp70 envelope proteins

Cited by 122 publications

References 16 publications

Inhibitors of monocyte responses to chemotaxins are present in human cancerous effusions and react with monoclonal antibodies to the P15(E) structural protein of retroviruses.

Inhibitors of monocyte responses to chemotaxins are present in human cancerous effusions and react with monoclonal antibodies to the P15(E) structural protein of retroviruses.

Ecotropic and Xenotropic Type C Retroviruses Associated with Reticulum Cell Neoplasms of SJL/J Mice

Acquisition of apparently intact and unmodified lipopolysaccharides from Escherichia coli by Bdellovibrio bacteriovorus

Contact Info

Product

Resources

About