Monoclonal antibodies against rat mast cells differentiate between subtypes

Repke, H; Rossow, N.; Sávoly, S.B.; Odarjuk, J.; Karawajew, Leonid; Gomes, J.C.

doi:10.1007/bf02074673

Cited by 6 publications

(2 citation statements)

References 2 publications

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“…No light microscopic staining differences were noted in these studies, and no ultrastructural studies were performed. These differences in guinea pig mast cells may correspond to the recently described heterogeneity of human mast cells based on ultrastructure, chymase content, monoclonal antibodies, and response to secretogogues (Arizono et al, 1987;Repke et al, 1987;Craig et al, 1988Craig et al, , 1989. This heterogeneity has not been studied in guinea pigs, but in humans both types of cells are present in all locations studied, although with different frequencies (Irani et al, 1986;Craig et al, 1989).…”

Section: Discussionmentioning

confidence: 81%

Eosinophils and mast cell homogeneity of the guinea pig eyelid skin, conjunctiva, and ileum

et al. 1989

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Mast cell heterogeneity has been described on the basis of differential staining reactions, light microscopic morphology, anatomic location, degranulation after polyamines, biochemical contents, growth requirements, and reactions to lymphokines. We have demonstrated typical "connective-tissue mast cells" by using anatomic criteria, histological staining reactions, electron microscopy, and reaction to compound 48/80 in the guinea pig conjunctiva, eyelid skin, and ileum. A second, much larger population of cells in the ileal mucosa and the conjunctiva, and rarely in the eyelid skin stained reddish-blue with acid toluidine blue in tissue fixed in ethanol-acetate-lead subacetate (BLA) and with alkaline Giemsa in formaldehyde-fixed tissue, did not stain with ethanolic or acid toluidine blue in formaldehyde-fixed tissue or with alkaline Giemsa in BLA-fixed tissue, and did not degranulate after 48/80 treatment. These are features of the rat intestinal "mucosal mast cells"; however, ultrastructural and light microscopic studies with the orcein Giemsa stain demonstrated these cells in the guinea pig to be eosinophils. Tissue culture, biochemical, and immunological studies indicate the existence of a second type of mast cell (bone-marrow-derived mast cell), ultrastructurally almost indistinguishable from the connective tissue mast cell. Our studies demonstrate only one mast cell type in the guinea pig and support the contention that other forms of mast cells are immature forms or variants of the connective-tissue mast cell.

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Section: Discussionmentioning

confidence: 81%

Eosinophils and mast cell homogeneity of the guinea pig eyelid skin, conjunctiva, and ileum

et al. 1989

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“…So, we tried to use a monoclonal antibody (MAb) able to recognize mast cells, which was produced in this laboratory [17], This MAb detects a membrane antigen, which is ex pressed by mast cells of the connective tissue type [18]. Blocking of this mast cell antigen by the MAb results in a dose-dependent inhibition of the compound 48/80-induced histamine release from mast cells [Odarjuk, unpubl.].…”

Section: Introductionmentioning

confidence: 99%

Investigation of Mast Cell Differentiation in vivo by Use of Monoclonal Antibodies

Odarjuk

Rossow

Karawajew

1989

Int Arch Allergy Immunol

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In the present study mast cell differentiation/maturation was studied in vivo after depletion of mature mast cells from the peritoneal cavity by injection of distilled water. The reconstituting cell population was characterized by use of different staining methods. Additionally, the monoclonal antibody (MAb) IWF F2, which recognizes a membrane antigen of rat mast cells, was used to follow up mast cell differentiation/maturation in the course of the experiment. The antigen expression was studied both by immunofluorescence of antigen-bearing cells and by MAb inhibition of compound 48/80-stimulated histamine release from mast cells. In the course of the experiment the amount of antigen-positive cells increased continuously from less than 5% to control level (1st and 22nd days, respectively). The expression of the membrane antigen detectable by the MAb precedes the appearance of cytochemically identifiable mast cells for several days. The mast cells mature morphologically and functionally as indicated by increasing size, histamine content and MAb inhibition of compound 48/80-stimulated histamine release. The results obtained suggest the MAb IWF F2 to be a useful methodical tool for additional characterization of mast cell differentiation/maturation processes.

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