Monoclonal antibodies against the presynaptic calcium channel antagonist ω‐conotoxin GVI A from cone snail poison

Abstract

Monoclonaf antibodies have been prepared against oxonotoxin GVI A, a peptide isolated from marine snails of the genus Conus ~Con~ge~ru~h~ a+ and Conus magus). This toxin is a blocker of select presynaptic Ca channels in the central nervous system. Antigenic o-conotoxin GVI A was synthesized as a covalent conjugate with bovine serum albumin and injected S.C. An ELISA assay combined with a competitive inhibition assay was used to select and characterize monoclonal antibodies able to recognize and bind the free toxin. Several of the antibodies were found to block w-conotoxin GVI A inhibition of %a transport into rat brain synaptosomes and to block w-conotoxin GVI A binding to membranes from the same preparation, The antibodies recognize native, synthetic toxin, and are useful for analysis of toxin in biological fluids.

Characterization of α-conotoxin interactions with the nicotinic acetylcholine receptor and monoclonal antibodies

“…Unlike mAbs against snake α-neurotoxins, which only delay the time to death [28,35], either α-conotoxin GI mAb was completely protective at a 1 : 1 molar ratio of αconotoxin to antibody. There is one report of mAbs developed against ω-conotoxin GVIA [36], a Ca# + channel antagonist found in C. geographus venom and consisting of 27 residues plus three disulphide bonds. Some of these mAbs prevent binding of ωconotoxin GVIA to rat brain synaptosomes.…”

Preparation and Identification of Monoclonal Antibodies Against ω-Conotoxin MVIIA

“…The MAbs against o-conotoxin GVIA were developed to recognize native and synthetic toxin and are useful for the analysis of toxin in tissue extracts or biological fluids. (10) It was also carried out to detect o-conotoxin MVIIA based on polyclonal antibody. (11) Thus far there still is not an immunoassay against o-CTX MVIIA based on MAb.…”